CLIA Kit for Interleukin 8 (IL8)Homo sapiens (Human)Sandwich CLIA

CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1

  • Product No.SCA080Hu
  • Organism SpeciesHomo sapiens (Human)Same name, Different species.
  • Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Test MethodDouble-antibody Sandwich
  • Assay Length2h, 40min
  • Detection Range15.62-1000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 0.57pg/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Interleukin 8 (IL8).
    No significant cross-reactivity or interference between Interleukin 8 (IL8) and analogues was observed.
  • ApplicationsChemiluminescent immunoassay for Antigen Detection
  • DownloadInstruction Manual
  • Format48T96T96T*596T*1096T*100
  • FOBUS$ 588US$ 840US$ 3780US$ 7140US$ 58800
  • CLIA Kit for Interleukin 8 (IL8)Package and Components
  • CLIA Kit for Interleukin 8 (IL8)Package and Components
  • CLIA Kit for Interleukin 8 (IL8)Results demonstration
  • SCA080Hu.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 8 (IL8) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 8 (IL8) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)90-10394
EDTA plasma(n=5)95-105102
heparin plasma(n=5)79-9793

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 8 (IL8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)90-98%82-92%80-104%98-105%
EDTA plasma(n=5)86-94%90-102%88-104%88-102%
heparin plasma(n=5)94-101%96-105%79-88%85-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
Substrate A1×10mLSubstrate B1×2mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Interleukin 8 (IL8)

Test principle

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 8 (IL8) level in the sample or standard.

Reference

  • Reproductive Biology and EndocrinologyEvidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cowRbej: 14777827
  • Acta OtolaryngolSerial cytokine levels during wound healing in rabbit maxillary sinus mucosaPubMed: 19958244
  • Journal of Clinical PeriodontologyExperimental peri-implant mucositis at different implant surfaces.Pubmed: 24521508
  • Physiological ResearchBudesonide added to modified porcine surfactant Curosurf may additionally improve the lung functions in meconium aspiration syndrome.Pubmed: 2432969
  • Rheumatology InternationalSerum levels of selected chemokines in systemic lupus erythematosus patientsPubmed: 22461186
  • J Med Life.Expression of interleukine-8 as an independent prognostic factor for sporadic colon cancer disseminationPubmed:Pmc4197484
  • J Clin Periodontol.Experimental peri‐implant mucositis at different implant surfacesPubmed:24521508
  • British Journal of NutritionGrape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental?×?Qinchuan steersPubmed:24846452
  • TheriogenologyInflammatory cytokine concentrations in uterine flush and serum samples from dairy cows with clinical or subclinical endometritisTheriojournal:Source
  • Anim Reprod Sci.Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranesPubMed: 25851495
  • Медицина и здравоохранениеДИНАМИКА ЦИТОКИНОВОГО ПРОФИЛЯ В ОЦЕНКЕ ЭФФЕКТИВНОСТИ РАЗНЫХ МЕТОДОВ СТИМУЛЯЦИИ РЕПАРАТИВНОГО ОСТЕОГЕНЕЗА В ЭКСПЕРИМЕНТЕArticle: N
  • BMC AnesthesiologyHyperinflation deteriorates arterial oxygenation and lung injury in a rabbit model of ARDS with repeated open endotracheal suctioningPubMed: 25943099
  • J Breath ResEffects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgeryPubMed: 25719610
  • ARTICLEThe Predictive Diagnostic and Prognostic Cut-off Values for Interleukin 8 in Patients with Meningitis in EgyptProfile: Mohmad_Mosaad
  • Veterinary Immunology and ImmunopathologyNeutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation periodPubmed:27090626
  • Infection, Genetics and EvolutionInfection of chicken bone marrow mononuclear cells with subgroup J avian leukosis virus inhibits dendritic cell differentiation and alters cytokine expressionPubmed:27349993
  • Asian Pacific Journal of Tropical MedicinePerfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injuryscience:S1995764516300955
  • Archives of Biological SciencesDiabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrolpublication:299492265_Diabetes-induced_renal_failu

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