CLIA Kit for Isocitrate Dehydrogenase 1, Soluble (IDH1)

PICD; IDP; Oxalosuccinate decarboxylase; NADP(+)-specific ICDH; Cytosolic NADP-isocitrate dehydrogenase
Typical Standard Curve Typical Standard Curve

Sample type Serum, plasma and other biological fluids Detection range 1.372-1000ng/mL The standard curve concentrations used for the ELISA's were 1000ng/mL, 333.33ng/mL, 111.11ng/mL, 37.04ng/mL, 12.346ng/mL, 4.115ng/mL, 1.372ng/mL
Sensitivity The minimum detectable dose of this kit is typically less than 0.47ng/mL. Specificity This assay has high sensitivity and excellent specificity for detection of Isocitrate Dehydrogenase 1, Soluble (IDH1).
No significant cross-reactivity or interference between Isocitrate Dehydrogenase 1, Soluble (IDH1) and analogues was observed.

Recovery Matrices listed below were spiked with certain level of recombinant Isocitrate Dehydrogenase 1, Soluble (IDH1) and the recovery rates were calculated by comparing the measured value to the expected amount of Isocitrate Dehydrogenase 1, Soluble (IDH1) in samples.
Matrix Recovery range (%) Average(%)
serum(n=5)78-9583
EDTA plasma(n=5)87-10598
heparin plasma(n=5)78-10486

Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Isocitrate Dehydrogenase 1, Soluble (IDH1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Isocitrate Dehydrogenase 1, Soluble (IDH1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Isocitrate Dehydrogenase 1, Soluble (IDH1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
serum(n=5)97-104%85-95%93-105%86-105%
EDTA plasma(n=5)94-105%86-101%92-99%83-103%
heparin plasma(n=5)88-105%95-105%79-91%87-102%

Stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Test principle The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Isocitrate Dehydrogenase 1, Soluble (IDH1) level in the sample or standard. Assay procedure summary 1. Prepare all reagents, samples and standards;
Reagent Preparation
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37oC;
8. Read RLU value immediately.

Reagents and materials provided
  • Pre-coated, ready to use 96-well strip plate1
  • Plate sealer for 96 wells4
  • Standard2
  • Standard Diluent1×20mL
  • Detection Reagent A1×120µL
  • Assay Diluent A1×12mL
  • Detection Reagent B1×120µL
  • Assay Diluent B1×12mL
  • Substrate A1×10mL
  • Substrate B1×2mL
  • Wash Buffer (30 × concentrate)1×20mL
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    CLIA Kit for Isocitrate Dehydrogenase 1, Soluble (IDH1) Material Safety Data Sheet International Organization for Standardization guarantee