Home  |   About us
ITEM PROTEIN ANTIBODY ASSAY KIT CELL
CLIA Kit for Leptin (LEP)
OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein
Organism species Homo sapiens (Human)
Product No.SCA084Hu
Sample typeTissue homogenates, cell lysates and other biological fluids
Format96-well strip plate
Assay length4.5 hours
Detection range
SensitivityThe minimum detectable dose of this kit is typically less than .
Specificity
This assay has high sensitivity and excellent specificity for detection of Leptin (LEP).
No significant cross-reactivity or interference between Leptin (LEP) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Leptin (LEP) and the recovery rates were calculated by comparing the measured value to the expected amount of Leptin (LEP) in samples.
Matrix Recovery range (%) Average(%)
serum(n=5)99-105102
EDTA plasma(n=5)88-9692
heparin plasma(n=5)80-10197
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leptin (LEP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leptin (LEP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Leptin (LEP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
serum(n=5)80-103%84-104%93-103%85-99%
EDTA plasma(n=5)95-104%98-105%97-105%84-98%
heparin plasma(n=5)82-101%79-101%83-91%87-95%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
Test principle
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Leptin (LEP) level in the sample or standard.
Package and Components
Assay procedure summary
1. Prepare all reagents, samples and     standards;
2. Add 100µL standard or sample to each     well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared     Detection Reagent A. Incubate 1 hour at     37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate     10 minutes at 37oC;
8. Read RLU value immediately.
Reagent Preparation
Results demonstration
Download
Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAA084Hu22Monoclonal Antibody to Leptin (LEP)WB, ICC, IHC-P, IHC-F, ELISA
PAA084Hu01Polyclonal Antibody to Leptin (LEP)WB, ICC, IHC-P, IHC-F, ELISA
PAA084Hu71Biotin-Linked Antibody to Leptin (LEP)IHC;WB;ELISA
PAA084Hu81FITC-Linked Antibody to Leptin (LEP)IHC;ICC;IF;WB
RPA084Hu01Recombinant Leptin (LEP)SDS-PAGE; WB; ELISA; IP.
SCA084HuCLIA Kit for Leptin (LEP)Chemiluminescent immunoassay
SEA084HuELISA Kit for Leptin (LEP)Enzyme-linked immunosorbent assay