CLIA Kit for Leptin (LEP) Homo sapiens (Human) Sandwich CLIA

OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

  • Product No.SCA084Hu
  • Organism SpeciesHomo sapiens (Human)
  • Sample TypeSerum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length2h, 40min
  • Detection Range468.75-30000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 15.1pg/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Leptin (LEP).
    No significant cross-reactivity or interference between Leptin (LEP) and analogues was observed.
  • ApplicationsChemiluminescent immunoassay for Antigen Detection
  • DownloadInstruction Manual
  • Format 48T96T 96T*5 96T*10 96T*100
  • FOB US$ 588US$ 840 US$ 3780 US$ 7140 US$ 58800
  • CLIA Kit for Leptin (LEP) Package and Components
  • CLIA Kit for Leptin (LEP) Package and Components
  • CLIA Kit for Leptin (LEP) Results demonstration
  • SCA084Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Leptin (LEP) and the recovery rates were calculated by comparing the measured value to the expected amount of Leptin (LEP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 99-105 102
EDTA plasma(n=5) 88-96 92
heparin plasma(n=5) 80-101 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leptin (LEP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leptin (LEP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Leptin (LEP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-103% 84-104% 93-103% 85-99%
EDTA plasma(n=5) 95-104% 98-105% 97-105% 84-98%
heparin plasma(n=5) 82-101% 79-101% 83-91% 87-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Leptin (LEP)

Test principle

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Leptin (LEP) level in the sample or standard.

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Reference

  • European Journal of PharmacologyPuerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat dietPubMed: 20869961
  • Indian Journal of PharmacologyEffect of Argyreia speciosa root extract on cafeteria diet-induced obesity in ratsIjp: source
  • Journal of endocrinologyTestosterone protects from metabolic syndrome-associated prostate inflammation: an experimental study in rabbitPubMed: 22010203
  • PLoS One.Multi-Functional Roles of Chitosan as a Potential Protective Agent against ObesityPubMed: 23342013
  • Molecular and Cellular EndocrinologySoy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigsScienceDirect: S0303720712004194
  • Journal of Animal and Feed SciencesOvarian, metabolic and endocrine indexes in dairy cows with different body condition scoresIfzz: Source
  • British Journal of Nutritionn-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigsPubmed: 23947577
  • Int J Mol Sci. Maternal high folic acid supplement promotes glucose intolerance and insulin resistance in male mouse offspring fed a high-fat diet.Pubmed: 24736781
  • Endocr J.CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytesPubmed:25168658
  • Animal PhysiologyEFFECT OF LEPTIN GENE ON SERUM LEPTIN CONCENTRATION AND BEEF QUALITY IN CZECH FLECKVIEH BULLSMendelu:Source
  • Evid Based Complement Alternat Med.Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar RatsPubmed:Pmc4363645
  • general endocrinologyOxytocin reverse ovariectomy-induced Osteoper and body fat gainPubmed:24506069
  • Respiratory Physiology &amp; NeurobiologyMaternal high-fat diet feeding during pregnancy and lactation augments lung inflammation and remodeling in the offspringPubmed:25500158
  • Sci RepHepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitisPubMed: 26634430
  • Evid Based Complement Alternat MedTinospora crispaPubMed: 25821506
  • Scientific ReportsBlood cell transcriptomic-based early biomarkers of adverse programming effects of gestational calorie restriction and their reversibility by leptin supplementationPubMed: 25766068
  • Ankara Effects of breeder age and early energy restriction on fattening performance, some meat quality traits and plasma leptin concentration of broilersDergiler: 11
  • MEDICINA VETERINComparative measurements of the morphometric pattern and energy profile of Crioula mares in the final third of gestation, with different body condition scoresScielo: S0102-09352015000300707
  • Pancreatic Disorders & Therapy Pancreatic Exocrine Insufficiency Affects Not Only Digestibility of Nutrients and Growth, but Also Body Composition and Endocrinological Parameters – Study on Piglets Used As a Model for Children Journals: 2165-7092-%20S5-00