CLIA Kit for Nitric Oxide Synthase 1, Neuronal (NOS1) Homo sapiens (Human) Sandwich CLIA

NOS; nNOS; IHPS1; NC-NOS; N-NOS; bNOS; Neuronal NOS; Constitutive NOS; NOS type I; Nitric Ocide Synthase; Peptidyl-cysteine S-nitrosylase NOS1

  • Product No.SCA815Hu
  • Organism SpeciesHomo sapiens (Human)
  • Sample TypeSerum, plasma, tissue homogenates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length2h, 40min
  • Detection Range156.25-10000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 6.03pg/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Nitric Oxide Synthase 1, Neuronal (NOS1).
    No significant cross-reactivity or interference between Nitric Oxide Synthase 1, Neuronal (NOS1) and analogues was observed.
  • ApplicationsChemiluminescent immunoassay for Antigen Detection
  • DownloadInstruction Manual
  • Format 48T96T 96T*5 96T*10 96T*100
  • FOB US$ 588US$ 840 US$ 3780 US$ 7140 US$ 58800
  • CLIA Kit for Nitric Oxide Synthase 1, Neuronal (NOS1) Package and Components
  • CLIA Kit for Nitric Oxide Synthase 1, Neuronal (NOS1) Package and Components
  • CLIA Kit for Nitric Oxide Synthase 1, Neuronal (NOS1) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Nitric Oxide Synthase 1, Neuronal (NOS1) and the recovery rates were calculated by comparing the measured value to the expected amount of Nitric Oxide Synthase 1, Neuronal (NOS1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-105 99
EDTA plasma(n=5) 95-103 99
heparin plasma(n=5) 93-101 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nitric Oxide Synthase 1, Neuronal (NOS1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nitric Oxide Synthase 1, Neuronal (NOS1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nitric Oxide Synthase 1, Neuronal (NOS1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-103% 97-104% 96-105% 78-93%
EDTA plasma(n=5) 98-105% 94-103% 99-105% 78-96%
heparin plasma(n=5) 90-104% 81-104% 88-102% 80-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Nitric Oxide Synthase 1, Neuronal (NOS1)

Test principle

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Nitric Oxide Synthase 1, Neuronal (NOS1) level in the sample or standard.

GIVEAWAYS

Reference

  • PLoS OneA Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric OxidePubMed: 23359814
  • J Endocrinol Invest.?The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disordersPubmed:25245337