CLIA Kit for Transthyretin (TTR) Homo sapiens (Human) Sandwich CLIA

TBPA; PA; PLB; ATTR; PALB; Prealbumin; Prealbumin Amyloidosis Type I; Transports Thyroxine and Retinol

  • CLIA Kit for Transthyretin (TTR) Packages (Simulation)
  • CLIA Kit for Transthyretin (TTR) Packages (Simulation)
  • CLIA Kit for Transthyretin (TTR) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Transthyretin (TTR) and the recovery rates were calculated by comparing the measured value to the expected amount of Transthyretin (TTR) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-103 99
EDTA plasma(n=5) 96-105 101
heparin plasma(n=5) 94-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transthyretin (TTR) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transthyretin (TTR) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transthyretin (TTR) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-92% 80-92% 78-97% 79-92%
EDTA plasma(n=5) 78-101% 79-96% 78-104% 78-91%
heparin plasma(n=5) 80-101% 91-98% 90-101% 80-94%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Transthyretin (TTR)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Transthyretin (TTR). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Transthyretin (TTR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Transthyretin (TTR) level in the sample or standard.;

Reference

  • Chinese Medical JournalScreening disease-associated proteins from sera of patients with rheumatoid arthritis: a comparative proteomic studyPubMed: 20367977
  • Journal of Vascular SurgeryCirculation levels of acute phase proteins in patients with Takayasu arteritisPubMed: 20100644
  • Journal of Pharmaceutical and Biomedical AnalysisEffect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2PubMed: 21130593
  • Toxicology LettersSerum proteomic analysis reveals potential serum biomarkers for occupational medicamentosa-like dermatitis caused by trichloroethylenePubmed:24960064
  • Journal of NeurochemistrySchwann cells contribute to neurodegeneration in transthyretin amyloidosisPubmed:25693163
  • Anal BiochemSurface plasmon resonance assay of inhibition by pharmaceuticals for thyroxine hormone binging to transport proteinsPubMed: 26384643
  • United States Patent ApplicationCompositions and Methods for Diagnosing Lung Cancery2016:0077095.html