ELISA Kit for Interferon Beta (IFNb) Bos taurus; Bovine (Cattle) Sandwich ELISA

IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast

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  • ELISA Kit for Interferon Beta (IFNb) Packages (Simulation)
  • ELISA Kit for Interferon Beta (IFNb) Packages (Simulation)
  • ELISA Kit for Interferon Beta (IFNb) Results demonstration
  • SEA222Bo.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Interferon Beta (IFNb) and the recovery rates were calculated by comparing the measured value to the expected amount of Interferon Beta (IFNb) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-105 101
EDTA plasma(n=5) 89-98 94
heparin plasma(n=5) 83-92 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Beta (IFNb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Beta (IFNb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interferon Beta (IFNb) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-103% 98-105% 96-103% 86-93%
EDTA plasma(n=5) 84-92% 88-102% 87-99% 80-101%
heparin plasma(n=5) 97-105% 89-98% 85-98% 92-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Interferon Beta (IFNb)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Beta (IFNb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Beta (IFNb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Beta (IFNb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Beta (IFNb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Melatonin protects liver against ischemia and reperfusion injury through inhibition of toll-like receptor signaling pathwayPubMed: 21355876
  • Hepatitis B virus polymerase inhibits RIG-I- and Toll-like receptor 3-mediated beta interferon induction in human hepatocytes through interference with interferon regulatory factor 3 activation and dampening of the interaction between TBK1/IKKε and DDX3.Sgm: 9182080
  • Impaired TLR3/IFN-β signaling in monocyte-derived dendritic cells from patients with acute-on-chronic hepatitis B liver failure: Relevance to the severity of liver damageScienceDirect: S0006291X09019822
  • Intact Type I Interferon Production and IRF7 Function in Sooty MangabeysPubMed: PMC3757038
  • Innate Immune Response to Rift Valley Fever Virus in GoatsPubMed: PMC3335883
  • Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar MacrophagesPubmed: 24288685
  • An attenuated EIAV strain and its molecular clone strain differentially induce the expression of Toll-like receptors and type-I interferons in equine monocyte-derived macrophages.Pubmed: 23850441
  • Scoparone attenuates d-galactosamine/lipopolysaccharide-induced fulminant hepatic failure through inhibition of toll-like receptor 4 signaling in micePubmed: 23535186
  • Experimental model of equine alveolar macrophage stimulation with TLR ligandsPubmed: 23815824
  • Training Modifies Innate Immune Responses in Blood Monocytes and in Pulmonary Alveolar MacrophagesPubmed: 24502337
  • Mannan binding lectin attenuates double-stranded RNA-mediated TLR3 activation and innate immunityPubmed:24530528
  • Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strainPubmed:25106750
  • MicroRNA-30e* Suppresses Dengue Virus Replication by Promoting NF-κB–Dependent IFN ProductionPubmed:25122182
  • The innate immune response of equine bronchial epithelial cells is altered by trainingPubmed:25595212
  • SOCS3 Drives Proteasomal Degradation of TBK1 and Negatively Regulates Antiviral Innate ImmunityPubMed: 25939384
  • Analysis of cytokine production in a newly developed canine tracheal epithelial cell line infected with H3N3 canine influenza virusPubMed: 25797196
  • Complexes of DNA with the Antimicrobial Peptide LL37 Augment NK Cell Functions by Inducing Type I Interferon Production from Circulating Monocytes and Plasmacytoid Predendritic CellsPubMed: 26258404
  • PKR activation enhances replication of classical swine fever virus in PK-15 cellsPubMed: 25899421
  • siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In VitroPubmed:26886455
  • Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4Pubmed:27090571
  • Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In VivoPubmed:27252695
  • Chloroquine attenuates lipopolysaccharide-induced inflammatory responses through upregulation of USP25.pubmed:28134560
  • Effects of IRF1 and IFN-β interaction on the M1 polarization of macrophages and its antitumor functionpubmed:27176664
  • Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cellsfiles:ijcep0036479.pdf
  • ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophagespubmed:27486255
  • Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys.pubmed:
  • MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis Bpubmed:28342861
  • The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infectionpubmed:28988058
  • Zika virus elicits inflammation to evade antiviral response by cleaving cGAS via NS1‐caspase‐1 axisPubmed:30065070
  • Bisphenol A induced male germ cell apoptosis via IFNβ-XAF1-XIAP pathway in adult micePubmed:30017639
  • MAVS induces a host cell defense to inhibit CSFV infectionPubmed:29556776
  • Inhibition of DNA-sensing pathway by Marek's disease virus VP23 protein through suppression of interferon regulatory factor 7 activationPubmed: 30518647
  • Molecular cloning and functional characterization of porcine 2′, 5′-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory?…Doi: 10.1016/j.vetimm.2019.01.003
  • HIPK2 is necessary for type I interferon–mediated antiviral immunityDoi: 10.1126/scisignal.aau4604
  • Evaluation of serum interferons in patients with age-related macular degenerationPubmed: 31007694
  • Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens
  • Porcine FcγRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transductionPubmed: 31284005
  • Marek's Disease Virus RLORF4 Inhibits Type I Interferon Production by Antagonizing NF-κB ActivationPubmed: 31243133
  • Serodiagnosis of Human Bocavirus 1 Infection among Hospitalised Children with Lower Respiratory Tract Infection in Latvia
  • The immunostimulatory effects and pro‐apoptotic activity of rhCNB against Lewis lung cancer is mediated by Toll‐like receptor 4Pubmed: 31218844
  • Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1
  • Avian oncogenic herpesvirus antagonizes the cGAS-STING DNA-sensing pathway to mediate immune evasionPubmed: 31539404
  • Induction of autophagy and suppression of type I IFN secretion by CSFVPubmed: 32160078
  • BVDV-1 induces interferon-beta gene expression through a pathway involving IRF1, IRF7, and NF-κB activationPubmed: 33053462
  • LDHB inhibition induces mitophagy and facilitates the progression of CSFV infectionPubmed: 32924761
  • Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathwayPubmed: 32532134
  • Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon …
  • Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance
  • Open reading frame 5 protein of porcine circovirus type 2 induces RNF128 (GRAIL) which inhibits mRNA transcription of interferon-β in porcine epithelial cells34416463
  • Anti‐IFN‐α/‐ω neutralizing antibodies from COVID‐19 patients correlate with downregulation of IFN response and laboratory biomarkers of disease severityPubmed:35419822
  • Chicken Intestinal Microbiota Modulation of Resistance to Nephropathogenic Infectious Bronchitis Virus Infection Through IFN-I

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