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ELISA Kit for Carbonic Anhydrase IX (CA9) Homo sapiens (Human) Sandwich ELISA

CA-IX; CAIX; MN; pMW1; P54/58N; Carbonic Dehydratase; Renal cell carcinoma-associated antigen G250; RCC-Associated Protein G250; Carbonate dehydratase IX

  • Product No.SED076Hu
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range7.81-500pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 2.9pg/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Carbonic Anhydrase IX (CA9).No significant cross-reactivity or interference between Carbonic Anhydrase IX (CA9) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
  • DownloadInstruction Manual
  • UOM 48T96T 96T*5 96T*10 96T*100 In stock
  • FOB US$ 319.00 US$ 455.00 US$ 2,048.00 US$ 3,868.00 US$ 31,850.00
  • Quantity
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  • ELISA Kit for Carbonic Anhydrase IX (CA9) Packages (Simulation)
  • ELISA Kit for Carbonic Anhydrase IX (CA9) Packages (Simulation)
  • ELISA Kit for Carbonic Anhydrase IX (CA9) Results demonstration
  • SED076Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Carbonic Anhydrase IX (CA9) and the recovery rates were calculated by comparing the measured value to the expected amount of Carbonic Anhydrase IX (CA9) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-103 96
EDTA plasma(n=5) 93-101 98
heparin plasma(n=5) 82-101 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbonic Anhydrase IX (CA9) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbonic Anhydrase IX (CA9) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Carbonic Anhydrase IX (CA9) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-97% 98-105% 90-97% 78-95%
EDTA plasma(n=5) 85-94% 79-99% 80-99% 88-102%
heparin plasma(n=5) 97-104% 81-101% 97-105% 88-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Carbonic Anhydrase IX (CA9)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase IX (CA9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase IX (CA9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase IX (CA9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbonic Anhydrase IX (CA9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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