TIG2; RARRES2; Tazarotene-Induced Gene 2; Retinoic Acid Receptor Responder 2,Tazarotene Induced; RAR-responsive protein TIG2
- Product No.SEA945Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.78 -50ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.32ng/mL.
- Specificity This assay has high sensitivity and excellent specificity for detection of Chemerin (CHEM).No significant cross-reactivity or interference between Chemerin (CHEM) and analogues was observed.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100 Out of stock
- FOB US$ 319.00 US$ 455.00 US$ 2,048.00 US$ 3,868.00 US$ 31,850.00
- Quantity Add to Cart Distributors
- Packages (Simulation)
- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Matrices listed below were spiked with certain level of recombinant Chemerin (CHEM) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemerin (CHEM) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemerin (CHEM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemerin (CHEM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemerin (CHEM) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemerin (CHEM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemerin (CHEM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemerin (CHEM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemerin (CHEM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Serum chemerin and vaspin in non-alcoholic fatty liver diseasePubMed: 20095887
- Chemerin, vaspin and insulin resistance in chronic hepatitis CWiley: source
- Expression and regulation of chemerin during rat pregnancyScienceDirect: S0143400412000902
- Chronic ethanol consumption increases the levels of chemerin in the serum and adipose tissue of humans and ratsNature: 201211
- Ankilozan spondilitli hastalarda adipositokin düzeyleri ve insülin direnciRaeddergisi: Source
- Association of chemerin with oxidative stress, inflammation and classical adipokines in non-diabetic obese patientsPubmed: 24702860
- Strong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjectsPubmed: 24303851
- Impact of OSA on Biological Markers in Morbid Obesity and Metabolic SyndromePubmed:24106991
- Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsiaPubmed:24840719
- Relationship between plasma chemerin levels and disease severity in COPD patientsPubmed:24865134
- Elevated serum chemerin in Chinese women with hyperandrogenic PCOSPubmed:24927079
- Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats.Pubmed:25471281
- Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty RatsPubmed:25697126
- Chemerin and calprotectin levels correlate with disease activity and inflammation markers in psoriasis vulgarisSciencedirect:S1027811714000470
- Serum Chemerin Levels in relation to Osteoporosis and Bone Mineral Density: A Case-Control StudyPubMed: 26170530
- Association of Serum Chemerin Levels with Acute Ischemic Stroke and Carotid Artery Atherosclerosis in a Chinese PopulationPubMed: 26471865
- Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant ratsPubMed: 25471281
- Effects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytesPubmed:26721419
- Association of chemerin levels and bone mineral density in Chinese obese postmenopausalwomen.pubmed:27583869