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ELISA Kit for Immunoglobulin G (IgG) Chicken (Gallus) Competition ELISA

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  • ELISA Kit for Immunoglobulin G (IgG) Packages (Simulation)
  • ELISA Kit for Immunoglobulin G (IgG) Packages (Simulation)
  • ELISA Kit for Immunoglobulin G (IgG) Results demonstration
  • CEA544Ga.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Immunoglobulin G (IgG) and the recovery rates were calculated by comparing the measured value to the expected amount of Immunoglobulin G (IgG) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-105 101
EDTA plasma(n=5) 89-96 93
heparin plasma(n=5) 98-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin G (IgG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 93-102% 88-104% 86-103%
EDTA plasma(n=5) 99-105% 97-105% 85-96% 81-98%
heparin plasma(n=5) 94-103% 81-98% 82-95% 91-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Immunoglobulin G (IgG)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Immunoglobulin G (IgG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Immunoglobulin G (IgG) and unlabeled Immunoglobulin G (IgG) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin G (IgG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin G (IgG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Immunoglobulin G (IgG) in the sample.

Citations

  • Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease VaccineMedwell: 2010610616
  • Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease VirusMedwell: 2010594600
  • EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHIIjfans: Source
  • Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88Pubmed: 24570422
  • Foot and Mouth Disease virus-loaded fungal chitosan nanoparticles for intranasal administration: impact of formulation on physicochemical and immunological characteristicsPubmed: 23590209
  • The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt SignalingHindawi:Source
  • Proteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infectionPubmed:24576640
  • Liver functions in silica-exposed workers in Egypt: possible role of matrix remodeling and immunological factorsPubmed:24999850
  • Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic micePubmed:25308407
  • Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancerPubmed:25529861
  • The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt SignalingPubMed: 26161394
  • Stabilization of Human Immunoglobulin G Encapsulated within Biodegradable Poly (Cyclohexane-1, 4-diyl Acetone Dimethylene Ketal) (PCADK)/ Poly (Lactic-co-Glycolic Acid) (PLGA) Blend MicrospheresPubMed: 26268563
  • Growth promotion in pigs by oxytetracycline coincides with down reCavia (Guinea pig )lation of serum inflammatory parameters and of hibernation‐associated protein HP‐27Pubmed:26914286
  • Neuropeptide Y stimulates osteoblastic differentiation and VEGF expression of bone marrow mesenchymal stem cells related to canonical Wnt signaling activating in vitro.Pubmed:26707636
  • Effect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestinepid:46641600021Z
  • INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATSjournals:3790
  • Expression of α-1,6-fucosyltransferase (FUT8) in rice grain and immunogenicity evaluation of plant-specific glycans.pubmed:28013072
  • Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system.pubmed:28220870
  • Effect of dietary protein or calcium supplement on the expression of collagen I and dentine phosphoprotein of rats with dental fluorosisC6TX00288A
  • Exposure of Piglets to Enteroviruses Investigated by an Immunoassay Based on the EV-G1 VP4 Peptidepubmed:27694750
  • Icariin promotes osteogenic differentiation of rat bone marrow stromal cells by activating the ERα-Wnt/β-catenin signaling pathway.pubmed:27764755
  • Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers10.108:09540105.2017.1311308
  • In vitro and in vivo immunomodulatory potential of Pedicularis longiflora and Allium carolinianum in alloxan-induced diabetes in ratspubmed:29091887
  • Fluoride or/and aluminum induced toxicity in guinea pig teeth with the low expression of dentine phosphoprotein10.1002/jbt.21912
  • Impact of Silver Nanoparticles on Immune Responses as Vaccine against Activated Staphylococcus aureus BacteriaISSN: 2319-7064
  • Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt.pubmed:28586019
  • Influence of eggshell colour on egg yolk antibody level, incubation results, and growth in broiler breeders10.1590/s1806-92902017000200004 
  • Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse modelPubmed:29655095
  • Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposisPubmed:30056130
  • Effects of and on growth performance, immune function and volatile fatty acid level of caecal digesta in broilers10.1080:09540105.2018.1457013
  • A Novel scFv Anti-Aβ Antibody Reduces Pathological Impairments in APP/PS1 Transgenic Mice via Modulation of Inflammatory Cytokines and Aβ-related EnzymesPubmed:30062438
  • Supplementation of Cr Methionine During Dry Period of Dairy Cows and Its Effect on Some Production and Biochemical Parameters During Early Lactation and on …Pubmed:29497999
  • Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a …Pubmed:29305686
  • Altered immunoglobulins (A and G) in Ghanaian patients with type 2 diabetesPubmed:29623201
  • Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipidsPubmed: 30237167
  • Fabrication and characterization of core-shell nanofibers using a next-generation airbrush for biomedical applicationsPubmed: 30433758
  • Phenotypic variations in transferred progeny due to genotype of surrogate mother: Surrogate mother modulates transferred progenyPubmed: 30445548
  • In vitro and in vivo cancer cell apoptosis triggered by competitive binding of Cinchona alkaloids to the RING domain of TRAF6
  • Serum exosomes from newborn piglets restrict porcine epidemic diarrhea virus infection
  • Ginsenoside Rg3 improves cyclophosphamide-induced immunocompetence in Balb/c micePubmed: 30974284
  • Utjecaj dodatka selena u prihrani na hematološku sliku i biokemijske pokazatelje u jelena lopatara (Dama dama L.)
  • Age-related chemokine alterations affect IgA secretion and gut immunity in female micePubmed: 32277312
  • Effects of polysaccharides from Yingshan Yunwu tea on meat quality, immune status and intestinal microflora in chickensPubmed: 32224178
  • Efficacy of Local Minocycline Agents in Treating Peri-Implantitis: An Experimental In Vivo Study in Beagle DogsPubmed: 33114168
  • Physicochemical characterization and immunomodulatory activity of sulfated Chinese yam polysaccharidePubmed: 33010421
  • Locally Applied Slow-Release of Minocycline Microspheres in the Treatment of Peri-Implant Mucositis: An Experimental In Vivo StudyPubmed: 32708741
  • Association Between Serum Selenium Level and Subclinical Mastitis in Dairy CattlePubmed: 32583225
  • Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanismPubmed: 33000198
  • Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo
  • Comparison of immunomodulatory effects of three polysaccharide fractions from Lentinula edodes water extracts
  • Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the …
  • A comparative study of autogenous, allograft and artificial bone substitutes on bone regeneration and immunotoxicity in rat femur defect model
  • Curcumin loaded nanostructure hybrid lipid capsules for co-eradication of breast cancer and cancer stem cells with enhanced anticancer efficacy
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  • A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate
  • Visualized podocyte-targeting and focused ultrasound responsive glucocorticoid nano-delivery system against immune-associated nephropathy without?¡­33456566
  • Celecoxib ameliorates liver cirrhosis via reducing inflammation and oxidative stress along spleen-liver axis in rats33577848
  • Handling Associated with Drenching Does Not Impact Survival and General Health of Low Birth Weight Piglets. Animals 2021, 11, 40433562568
  • Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure34090073
  • Optimized GAPDH-truncated immunogen of Streptococcus equi elicits an enhanced immune response and provides effective protection in a mouse model33647714
  • Insulin-like growth factor-1 improves postoperative cognitive dysfunction following splenectomy in aged rats33574912
  • The suppression of Brd4 inhibits peripheral plasma cell differentiation and exhibits therapeutic potential for systemic lupus erythematosus
  • A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle
  • Neonatal Buza??larda ?ki Farkl? ?mmunomod¨¹lat?r¨¹n Buza?? ?shalleri ¨¹zerine Etkileri
  • Dietary Supplementation of Inulin Ameliorates Subclinical Mastitis via Regulation of Rumen Microbial Community and Metabolites in Dairy Cows34494854
  • Prenatal diagnosis of maternal serum from mothers carrying β‐thalassemic fetus34559910
  • Protection from Benzene-induced Immune Dysfunction in MicePubmed:35090963
  • Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein ComponentsPubmed:35438093
  • Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell deathPubmed:35732625
  • Integrative epigenomic and transcriptomic analysis reveals the requirement of JUNB for hematopoietic fate inductionPubmed:35668082
  • The effect of the multi-strain probiotic preparation EM Bokashi® on selected parameters of the cellular immune response in pigs
  • 信筒子醌对系统性红斑狼疮小鼠免疫调节和治疗作用

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