ELISA Kit for Anti-Albumin Antibody (Anti-ALB)Homo sapiens (Human)Antibody ELISA

Serum albumin

  • Product No.AEB028Hu
  • Organism SpeciesHomo sapiens (Human)Same name, Different species.
  • Sample TypeSerum, plasma and other biological fluids.
  • Test MethodDouble-antibody Sandwich
  • Assay Length2h, 30min
  • Detection Range3.12-200ng/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 1.27ng/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Albumin (ALB).
    No significant cross-reactivity or interference between Albumin (ALB) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antibody Detection
  • DownloadInstruction Manual
  • Format48T96T96T*596T*1096T*100
  • FOBUS$ 560US$ 800US$ 3600US$ 6800US$ 56000
  • ELISA Kit for Anti-Albumin Antibody (Anti-ALB)Package and Components
  • ELISA Kit for Anti-Albumin Antibody (Anti-ALB)Package and Components
  • ELISA Kit for Anti-Albumin Antibody (Anti-ALB)Results demonstration
  • AEB028Hu.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Albumin (ALB) and the recovery rates were calculated by comparing the measured value to the expected amount of Albumin (ALB) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)79-9387
EDTA plasma(n=5)92-10197
heparin plasma(n=5)80-103101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Albumin (ALB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Albumin (ALB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Albumin (ALB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)89-103%94-102%83-103%80-91%
EDTA plasma(n=5)85-97%95-105%89-102%93-105%
heparin plasma(n=5)87-101%91-101%81-103%90-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Anti-Albumin Antibody (Anti-ALB)

Test principle

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Albumin (ALB) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Albumin (ALB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reference

  • PLoS OneSimvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical FormationPubMed: 22701728
  • Liver InternationalAlbumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pupsOnlinelibrary: liv.12219
  • Journal of Infectious DiseasesAdjunctive corticosteroid therapy improves lung immunopathology and survival during severe secondary pneumococcal pneumonia in micePubmed: 24273183
  • Toxins (Basel).Increased Circulating Levels of Vitamin D Binding Protein in MS PatientsPubmed:25590278
  • Mol Cell BiochemThe wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN micePubMed: 26346164