ELISA Kit for Bcl2 Associated X Protein (Bax) Homo sapiens (Human) Sandwich ELISA

BCL2L4; Bax Zeta; Apoptosis regulator BAX; Bcl-2-like protein 4

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  • ELISA Kit for Bcl2 Associated X Protein (Bax) Packages (Simulation)
  • ELISA Kit for Bcl2 Associated X Protein (Bax) Packages (Simulation)
  • ELISA Kit for Bcl2 Associated X Protein (Bax) Results demonstration
  • SEB343Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Bcl2 Associated X Protein (Bax) and the recovery rates were calculated by comparing the measured value to the expected amount of Bcl2 Associated X Protein (Bax) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-105 96
EDTA plasma(n=5) 86-94 90
heparin plasma(n=5) 79-92 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2 Associated X Protein (Bax) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2 Associated X Protein (Bax) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bcl2 Associated X Protein (Bax) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-92% 80-96% 78-92% 99-105%
EDTA plasma(n=5) 94-102% 80-98% 85-99% 94-101%
heparin plasma(n=5) 91-103% 90-97% 88-95% 93-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Bcl2 Associated X Protein (Bax)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Associated X Protein (Bax). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Associated X Protein (Bax). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Associated X Protein (Bax), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2 Associated X Protein (Bax) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Neurotoxic effect of lead on rats: Relationship to ApoptosisNCBI: PMC3883608
  • Effects of hepatocyte growth factor in myocarditis rats induced by immunization with porcine cardiac myosinPubmed: 24327573
  • Cu (II) (PICOLINYL-L-TRYPTOPHAN)2 AS AN EFFECIENT MULTIFUNCTIONAL RADIOPROTECTORAsj-oa: Source
  • Inhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritisPubmed:25511416
  • The combined treatment with novel platinum(II) complex and anti-MUC1 increases apoptotic response in MDA-MB-231 breast cancer cellsPubMed: 26112902
  • Inhibition of small HA fragment activity and stimulation of A2APubMed: 25511416
  • Hepatoprotective Effects of Rubus idaeus L. Leaves against CCl4-Induced Liver Injury via Antioxidant, Anti-inflammatory and Antiapoptotic Mechanismsopenview:626440
  • In Vivo Characterization of ARN14140, a Memantine/Galantamine-Based Multi-Target Compound for Alzheimer's Diseasepubmed:27609215
  • An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's …articles:journal-of-alzheimers-disease
  • Amyloid toxicity is enhanced after pharmacological or genetic invalidation of the 1 receptor pubmed:29129596
  • Mechanistic insights into the effects of quercetin and/or GLP-1 analogue liraglutide on high-fat diet/streptozotocin-induced type 2 diabetes in ratspubmed:28554128
  • Mechanism of anticancer action of novel berenil complex of platinum(II) combined with anti-MUC1 in MCF-7 breast cancer cells10.3892/ol.2017.7623
  • A polysaccharide (PNPA) from Pleurotus nebrodensis ameliorates hepatic ischemic/reperfusion (I/R) injury in ratspubmed:28709897
  • Potentiation of Apoptosis in Rat Striatum Exposed to Endosulfan and the Role of Quercetin10.1007/s13530-017-0325-1
  • Cardioprotective effect of the xanthones from Gentianella acuta against myocardial ischemia/reperfusion injury in isolated rat heartpubmed:28686977
  • Viscum articulatum Burm. f. aqueous extract exerts antiproliferative effect and induces cell cycle arrest and apoptosis in leukemia cellsPubmed:29555410
  • Mechanism of anticancer action of novel berenil complex of platinum (II) combined with anti-MUC1 in MCF-7 breast cancer cellsPubmed:29434943
  • Association of Bax and Bcl-2 Functional Polymorphisms and Protein Levels with Posttraumatic Stress DisorderpaperID:82598
  • Taxifolin protects rat against myocardial ischemia/reperfusion injury by modulating the mitochondrial apoptosis pathwayPubmed: 30723634
  • INSTITUTO DE CIENCIAS BÁSICAS Y PRECLÍNICAS “VICTORIA DE GIRÓN” DEPARTAMENTO DE HISTOLOGÍA
  • LncRNA SNHG3 promotes clear cell renal cell carcinoma proliferation and migration by upregulating TOP2APubmed: 31505165
  • LCZ696 (sacubitril/valsartan) protects against cyclophosphamide-induced testicular toxicity in rats: Role of neprilysin inhibition and lncRNA TUG1 in ameliorating …Pubmed: 32197949
  • Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cellsPubmed: 33206337
  • Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low− dose of …Pubmed: 32915453
  • Alogliptin attenuates cyclophosphamide-induced nephrotoxicity: a novel therapeutic approach through modulating MAP3K/JNK/SMAD3 signaling cascadePubmed: 32869669
  • Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats
  • Potential effect of adrenomedullin on metabolic and endocrinal dysfunctions in the experimentally induced polycystic ovary: Targeting implication of endoplasmic ¡­33491863
  • Potential Therapeutic Effects of New Ruthenium (III) Complex with Quercetin: Characterization, Structure, Gene Regulation, and Antitumor and Anti-Inflammatory Studies (RuIII/Q Novel Complex Is a Potent Immunoprotective Agent)
  • Allicin ameliorates renal ischemia/reperfusion injury via inhibition of oxidative stress and inflammation in rats34426252
  • Galangin mitigates oxidative stress, inflammation, and apoptosis in a rat model of methotrexate hepatotoxicity34729716
  • Selenium nanoparticles overcomes sorafenib resistance in thioacetamide induced hepatocellular carcinoma in rats by modulation of mTOR, NF-κB pathways and …Pubmed:35640776
  • Piracetam attenuates cyclophosphamide-induced hepatotoxicity in rats: Amelioration of necroptosis, pyroptosis and caspase-dependent apoptosisPubmed:35636581

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