ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)Homo sapiens (Human)Sandwich ELISA

BMP2A; BMP-2A; Hemochromatosis Modifier

  • Product No.SEA013Hu
  • Organism SpeciesHomo sapiens (Human)Same name, Different species.
  • Sample Typeserum, plasma, tissue homogenates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range62.5-1000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 25.1pg/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 2 (BMP2).
    No significant cross-reactivity or interference between Bone Morphogenetic Protein 2 (BMP2) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection
  • DownloadInstruction Manual
  • Format48T96T96T*596T*1096T*100
  • FOBUS$ 441US$ 630US$ 2835US$ 5355US$ 44100
  • ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)Package and Components
  • ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)Package and Components
  • ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)Results demonstration
  • SEA013Hu.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Bone Morphogenetic Protein 2 (BMP2) and the recovery rates were calculated by comparing the measured value to the expected amount of Bone Morphogenetic Protein 2 (BMP2) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)88-10299
EDTA plasma(n=5)87-105101
heparin plasma(n=5)83-9186

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 2 (BMP2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bone Morphogenetic Protein 2 (BMP2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)97-105%82-92%89-97%79-97%
EDTA plasma(n=5)80-95%94-101%91-105%82-104%
heparin plasma(n=5)81-101%82-90%87-95%94-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 2 (BMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 2 (BMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 2 (BMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein 2 (BMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reference

  • Journal of Applied ToxicologyChronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in miceWiley: Source
  • PLoS ONEA Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
  • Medical Journal of Chinese People&#039;s Liberation ArmyEffect of transplantation of BMP-2-induced bone marrow mesenchymal stem cells on myocardial infarction of rats after reperfusionSource
  • PLOS ONEPDGF-AA promotes osteogenic differentiation and migration of mesenchymal stem cell by down-regulating PDGFRα and derepressing BMP-Smad1/5/8 signalingPubmed:25470749
  • Eur J PharmacolNegative effect of serotonin–norepinephrine reuptake inhibitor therapy on rat bone tissue after orchidectomyPubMed: 25934570
  • PharmacologyEffect of Mirtazapine on Rat Bone Tissue after OrchidectomyPubMed: 25871861
  • Journal of Cellular BiochemistryTitanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling PathwayPubMed: 26681207