ELISA Kit for Chemerin (CHEM) Homo sapiens (Human) Sandwich ELISA

TIG2; RARRES2; Tazarotene-Induced Gene 2; Retinoic Acid Receptor Responder 2,Tazarotene Induced; RAR-responsive protein TIG2

  • Product No.SEA945Hu
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range0.156-10ng/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 0.069ng/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Chemerin (CHEM).No significant cross-reactivity or interference between Chemerin (CHEM) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
  • DownloadInstruction Manual
  • UOM 48T96T 96T*5 96T*10 96T*100 Ensure in stock; if not, free redo!
  • FOB US$ 441.00 US$ 630.00 US$ 2,835.00 US$ 5,355.00 US$ 44,100.00
  • Quantity
  • Add to Cart Distributors
  • ELISA Kit for Chemerin (CHEM) Packages (Simulation)
  • ELISA Kit for Chemerin (CHEM) Packages (Simulation)
  • ELISA Kit for Chemerin (CHEM) Results demonstration
  • SEA945Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Chemerin (CHEM) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemerin (CHEM) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-101 95
EDTA plasma(n=5) 97-104 101
heparin plasma(n=5) 89-97 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemerin (CHEM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemerin (CHEM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemerin (CHEM) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 95-103% 98-105% 78-101%
EDTA plasma(n=5) 92-101% 92-101% 86-105% 97-104%
heparin plasma(n=5) 88-97% 78-91% 95-102% 94-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Chemerin (CHEM)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemerin (CHEM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemerin (CHEM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemerin (CHEM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemerin (CHEM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reference

  • Scandinavian Journal of GastroenterologySerum chemerin and vaspin in non-alcoholic fatty liver diseasePubMed: 20095887
  • Gastroenterology & HepatologyChemerin, vaspin and insulin resistance in chronic hepatitis CWiley: source
  • PlacentaExpression and regulation of chemerin during rat pregnancyScienceDirect: S0143400412000902
  • Acta Pharmacologica SinicaChronic ethanol consumption increases the levels of chemerin in the serum and adipose tissue of humans and ratsNature: 201211
  • RAED dergisiAnkilozan spondilitli hastalarda adipositokin düzeyleri ve insülin direnciRaeddergisi: Source
  • Journal of Cellular and Molecular MedicineAssociation of chemerin with oxidative stress, inflammation and classical adipokines in non-diabetic obese patientsPubmed: 24702860
  • Clinical EndocrinologyStrong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjectsPubmed: 24303851
  • Oral Dis. Impact of OSA on Biological Markers in Morbid Obesity and Metabolic SyndromePubmed:24106991
  • EndocrineChemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsiaPubmed:24840719
  • The Clinical Respiratory JournalRelationship between plasma chemerin levels and disease severity in COPD patientsPubmed:24865134
  • Gynecol Endocrinol.Elevated serum chemerin in Chinese women with hyperandrogenic PCOSPubmed:24927079
  • Endocrine.Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats.Pubmed:25471281
  • Obesity SurgeryLong-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty RatsPubmed:25697126
  • Dermatologica SinicaChemerin and calprotectin levels correlate with disease activity and inflammation markers in psoriasis vulgarisSciencedirect:S1027811714000470
  • Dis MarkersSerum Chemerin Levels in relation to Osteoporosis and Bone Mineral Density: A Case-Control StudyPubMed: 26170530
  • Med Sci MonitAssociation of Serum Chemerin Levels with Acute Ischemic Stroke and Carotid Artery Atherosclerosis in a Chinese PopulationPubMed: 26471865
  • EndocrineProtective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant ratsPubMed: 25471281
  • Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsEffects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytesPubmed:26721419
  • Medicine (Baltimore).Association of chemerin levels and bone mineral density in Chinese obese postmenopausalwomen.pubmed:27583869

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