ELISA Kit for Galanin (GAL) 
GALN; GLNN; GMAP; Galanin Prepropeptide; Galanin-Message-Associated Peptide
- UOM
- FOB US$ 466.00 US$ 665.00 US$ 2,993.00 US$ 5,653.00 US$ 46,550.00
- Quantity
Overview
Properties
- Product No.CEB084Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathwayEndocrinologyReproductive scienceNeuro scienceHepatologyGastroenterologyHormone metabolism
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Galanin (GAL) and the recovery rates were calculated by comparing the measured value to the expected amount of Galanin (GAL) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 88-97 | 92 |
| EDTA plasma(n=5) | 90-97 | 94 |
| heparin plasma(n=5) | 90-99 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galanin (GAL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galanin (GAL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Galanin (GAL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-94% | 90-98% | 91-101% | 88-99% |
| EDTA plasma(n=5) | 84-97% | 88-96% | 88-102% | 88-97% |
| heparin plasma(n=5) | 78-94% | 95-103% | 85-99% | 95-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Galanin (GAL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Galanin (GAL) and unlabeled Galanin (GAL) (Standards or samples) with the pre-coated antibody specific to Galanin (GAL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Galanin (GAL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Galanin (GAL) in the sample.
Giveaways
Increment services
Citations
- Decreased galanin serum levels are associated with alcohol-craving during withdrawalScienceDirect: S0278584610005191
- Circulating galanin levels are increased in patients with gestational diabetes mellitusPubMed: 23266294
- Endogenous galanin as a novel biomarker to predict gestational diabetes mellitus.Pubmed: 24503374
- Circulating galanin and IL-6 concentrations in gestational diabetes mellitusPubmed:24397394
- Association between circulating levels of galanin and pre-pregnancy body mass index in patients with gestational diabetes mellitusPubMed: 26172564
- The Effects of Levetiracetam on Cerebrospinal Fluid and Plasma NPY and GAL, and on the Components of Stress Response System, hs-CRP, and S100B Protein in Serum of Patients with Refractory EpilepsyArticle: 10.1007
- Low Galanin Serum Levels are Associated with Anxiety in Opioid-Maintained Patients688687ca8d5a1d03ed060f761328001ca673.pdf
- Colorectal cancer patients exhibit increased levels of galanin in serum and colon tissuespubmed:27899999
- Serum Galanin Levels in Young Healthy Lean and Obese Non-Diabetic Men during an OralGlucose Tolerance Test.pubmed:27550417
- Adrenomedullin and Galanin Responses to Orthostasis in Older Persons pubmed:28796366
- Serum Galanin in Children with Autism Spectrum Disorder33534034