ELISA Kit for Growth Hormone (GH) Homo sapiens (Human) Sandwich ELISA

GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal

  • Product No.SEA044Hu
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range0.156-10ng/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 0.059ng/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Growth Hormone (GH).No significant cross-reactivity or interference between Growth Hormone (GH) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
  • DownloadInstruction Manual
  • UOM 48T96T 96T*5 96T*10 96T*100 Ensure in stock; if not, free redo!
  • FOB US$ 525.00 US$ 750.00 US$ 3,375.00 US$ 6,375.00 US$ 52,500.00
  • Quantity
  • Add to Cart Distributors
  • ELISA Kit for Growth Hormone (GH) Packages (Simulation)
  • ELISA Kit for Growth Hormone (GH) Packages (Simulation)
  • ELISA Kit for Growth Hormone (GH) Results demonstration
  • SEA044Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Growth Hormone (GH) and the recovery rates were calculated by comparing the measured value to the expected amount of Growth Hormone (GH) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-101 90
EDTA plasma(n=5) 94-103 98
heparin plasma(n=5) 90-104 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone (GH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone (GH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Growth Hormone (GH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-102% 79-90% 89-102% 92-105%
EDTA plasma(n=5) 89-96% 78-93% 87-96% 80-90%
heparin plasma(n=5) 87-96% 84-94% 87-101% 94-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Growth Hormone (GH)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Hormone (GH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Hormone (GH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Hormone (GH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Hormone (GH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reference

  • Neuroscience LettersThe effect of peripheral administration of growth hormone on AD-like cognitive deficiency in NBM-lesioned ratsPubMed: 19765635
  • Cellular ReprogrammingChanges in the Somatotrophic Axis in Genetically Identical DogsPubMed: 21740269
  • Clinical Cancer ResearchThe efficacy of IGF-I receptor monoclonal antibody against human gastrointestinal carcinomas is independent of k-ras mutation statusPubMed: 21642381
  • General and Comparative EndocrinologyGrowth hormone expression and neuroprotective activity in a quail neural retina cell lineScienceDirect: S0016648009002457
  • Nutrition and MetabolismPeriodic 48 h feed withdrawal improves glucose tolerance in growing pigs by enhancing adipogenesis and lipogenesisNutrition&Metabolism: source
  • Veterinary Recprd Case ReportTreatment of cervical metaphyseal fractures due to delayed ossification in an adult dog with hypopituitarismBmj: Source
  • Journal of the American Animal Hospital AssociationGH Deficiency in a Dalmatian Puppy with MegaesophagusPubmed: 24051259
  • Biotechnology and Bioprocess Engineering Functional expression of bovine growth hormone gene in Pleurotus eryngiiSpringer: Source
  • Buletin Veteriner UdayanaProfil Growth Hormone Sapi Bali Betina Pada Tiga Tipe Lahan Pemeliharaan Di Provinsi BaliUnud:Source
  • Veterinary WorldEffect of prill fat supplementation on hormones, milk production and energy metabolites during mid lactation in crossbred cowsVeterinaryworld:Source
  • Int J Clin Exp MedEffects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetesPubMed: 26379831
  • Acta HistochemicaMelatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicksPubmed:26948666
  • ANNALS OF ANIMAL SCIENCEComparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87
  • Annals of Animal ScienceComparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickensview:87
  • Journal of Dairy ScienceEffect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calvespublication:306322503
  • Jurnal Riset AkuakulturTRANSMISI GEN PhGH DAN PERFORMA PERTUMBUHAN IKAN LELE AFRIKA (Clarias gariepinus) TRANSGENIK GENERASI KETIGA1329

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