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ELISA Kit for Lipase, Hepatic (LIPC)
HL; HTGL; Hepatic Lipase; Hepatic triacylglycerol lipase; Lipase member C
Organism species Homo sapiens (Human)
Product No.SEA769Hu
Sample typeSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
Format96-well strip plate
Assay length4.5 hours
Detection range78.125-5000pg/mL The standard curve concentrations used for the ELISA’s were 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312.5pg/mL, 156.25pg/mL, 78.125pg/mL
SensitivityThe minimum detectable dose of this kit is typically less than 33pg/mL.
This assay has high sensitivity and excellent specificity for detection of Lipase, Hepatic (LIPC).
No significant cross-reactivity or interference between Lipase, Hepatic (LIPC) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Lipase, Hepatic (LIPC) and the recovery rates were calculated by comparing the measured value to the expected amount of Lipase, Hepatic (LIPC) in samples.
Matrix Recovery range (%) Average(%)
EDTA plasma(n=5)92-10197
heparin plasma(n=5)85-9995
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase, Hepatic (LIPC) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase, Hepatic (LIPC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lipase, Hepatic (LIPC) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
EDTA plasma(n=5)99-105%91-99%91-101%96-105%
heparin plasma(n=5)90-98%91-101%96-104%84-95%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Hepatic (LIPC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase, Hepatic (LIPC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Hepatic (LIPC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase, Hepatic (LIPC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Package and Components
Assay procedure summary
1. Prepare all reagents, samples and
2. Add 100µL standard or sample to each
    well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared
    Detection Reagent A. Incubate 1 hour at
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent
    B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15
    -25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm
Reagent Preparation
Results demonstration
Typical Standard Curve
Benchmark price
The American Journal of Clinical Nutrition Randomized controlled study of the effect of a butter naturally enriched in trans fatty acids on blood lipids in healthy women1    Read more(Ajcn: source)
J Lipid Res. Effect of weight loss, independent of change in diet composition, on apolipoprotein AI kinetic in men with metabolic syndrome    Read more(Pubmed: 23125458)
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAA769Hu22Monoclonal Antibody to Lipase, Hepatic (LIPC)WB, ICC, IHC-P, IHC-F, ELISA
PAA769Hu01Polyclonal Antibody to Lipase, Hepatic (LIPC)WB, ICC, IHC-P, IHC-F, ELISA
PAA769Hu02Polyclonal Antibody to Lipase, Hepatic (LIPC)WB, ICC, IHC-P, IHC-F, ELISA
PAA769Hu71Biotin-Linked Antibody to Lipase, Hepatic (LIPC)IHC;WB;ELISA
RPA769Hu01Recombinant Lipase, Hepatic (LIPC)SDS-PAGE; WB; ELISA; IP.
RPA769Hu02Recombinant Lipase, Hepatic (LIPC)SDS-PAGE; WB; ELISA; IP.
SEA769HuELISA Kit for Lipase, Hepatic (LIPC)Enzyme-linked immunosorbent assay