Home  |   About us
ITEM PROTEIN ANTIBODY ASSAY KIT CELL
ELISA Kit for Interleukin 6 (IL6)
MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
Organism species Homo sapiens (Human)
Product No.SEA079Hu
Sample typeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Format96-well strip plate
Assay length4.5 hours
Detection range1.563-100pg/mL The standard curve concentrations used for the ELISA’s were 100pg/mL, 50pg/mL, 25pg/mL, 12.5pg/mL, 6.25pg/mL, 3.125pg/mL, 1.563pg/mL
SensitivityThe minimum detectable dose of this kit is typically less than 0.61pg/mL.
Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 6 (IL6).
No significant cross-reactivity or interference between Interleukin 6 (IL6) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Interleukin 6 (IL6) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 6 (IL6) in samples.
Matrix Recovery range (%) Average(%)
serum(n=5)84-9789
EDTA plasma(n=5)92-10297
heparin plasma(n=5)85-9893
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 6 (IL6) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
serum(n=5)78-102%97-104%93-101%96-105%
EDTA plasma(n=5)99-105%89-98%93-101%95-102%
heparin plasma(n=5)99-105%95-103%87-103%78-103%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Package and Components
Assay procedure summary
1. Prepare all reagents, samples and
    standards;
2. Add 100µL standard or sample to each
    well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared
    Detection Reagent A. Incubate 1 hour at
    37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent
    B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15
    -25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm
    immediately.
Reagent Preparation
Results demonstration
Typical Standard Curve
Download
Benchmark price
MagazineReference
Polish Journal of Veterinary Sciences The effect of different dietary levels of vitamin E and selenium on antioxidant status and immunological markers in serum of laying hens    Read more(Pubmed: 23971202)
The Veterinary Journal Monocyte chemotactic protein-1 and other inflammatory parameters in Bernese Mountain dogs with disseminated histiocytic sarcoma    Read more(Pubmed: 23992872)
Not available Analysis of genetic markers for cardiovascular disorders in a portuguese population with familial hypercholesterolemia    Read more(Universi: 49434630)
Acta Otolaryngol Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa    Read more(PubMed: 19958244)
Reproductive BioMedicine Online Soluble BAFF-R produced by decidual stromal cells plays an inhibitory role in monocytes and macrophages     Read more(ScienceDirect: S1472648312001484)
Carcinogenesis The association of interleukin-16 polymorphisms with IL-16 serum levels and risk of colorectal and gastric cancer    Read more(OxfordJournals: source)
Diabetes Research and Clinical Practice Plasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistance    Read more(PubMed: 23290274)
PLoS One. Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome Sequencing    Read more(PubMed: PMC3378547)
6th International Scientific Interdisciplinary Conference (ISIC) Inflammation influence on the formation of endothelial dysfunction in hypertensive patients caused by obesity    Read more(Knmu: Source)
Glob J Health Sci. Inflammatory markers and haptoglobin polymorphism in Saudi with non-insulin-dependent diabetes mellitus.    Read more(Pubmed: 23283045)
Inflamm Res. Intracranial injection of recombinant stromal-derived factor-1 alpha (SDF-1α) attenuates traumatic brain injury in rats.    Read more(Pubmed: 24352531)
J Surg Res. Immunogenic alteration in laparoscopic common bile duct exploration.    Read more(Pubmed: 24239148)
Cardiovascular disease clinical research THE CHANGE OF SERUM LEVEL OF PIGMENT EPITHELIUM-DERIVED FACTOR IN CORONARY HEART DISEASE    Read more(Bmj: Source)
Journal of Ethnopharmacology Bioactive compounds from Cornus officinalis fruits and their effects on diabetic nephropathy    Read more(ScienceDirect: S0378874114002414)
Biosensors and Bioelectronics A new signal amplification strategy of photoelectrochemical immunoassay for highly sensitive interleukin-6 detection based on TiO2/CdS/CdSe dual co-sensitized structure    Read more(ScienceDirect: S0956566314001924)
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
HEA079HuHigh Sensitive ELISA Kit for Interleukin 6 (IL6)Enzyme-linked immunosorbent assay
MAA079Hu22Monoclonal Antibody to Interleukin 6 (IL6)WB, ICC, IHC-P, IHC-F, ELISA
PAA079Hu01Polyclonal Antibody to Interleukin 6 (IL6)WB, ICC, IHC-P, IHC-F, ELISA
PAA079Hu71Biotin-Linked Antibody to Interleukin 6 (IL6)IHC;WB;ELISA
RPA079Hu01Recombinant Interleukin 6 (IL6)SDS-PAGE; WB; ELISA; IP.
SCA079HuCLIA Kit for Interleukin 6 (IL6)Chemiluminescent immunoassay
SEA079HuELISA Kit for Interleukin 6 (IL6)Enzyme-linked immunosorbent assay