ELISA Kit for Interleukin 8 (IL8) Homo sapiens (Human) Sandwich ELISA

CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1

  • ELISA Kit for Interleukin 8 (IL8) Packages (Simulation)
  • ELISA Kit for Interleukin 8 (IL8) Packages (Simulation)
  • ELISA Kit for Interleukin 8 (IL8) Results demonstration
  • SEA080Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 8 (IL8) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 8 (IL8) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-102 96
EDTA plasma(n=5) 79-91 87
heparin plasma(n=5) 97-104 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 8 (IL8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-101% 79-93% 97-105% 95-103%
EDTA plasma(n=5) 93-101% 86-103% 80-91% 82-103%
heparin plasma(n=5) 80-101% 81-93% 78-93% 96-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Interleukin 8 (IL8)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 8 (IL8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 8 (IL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 8 (IL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 8 (IL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reference

  • Reproductive Biology and EndocrinologyEvidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cowRbej: 14777827
  • Acta OtolaryngolSerial cytokine levels during wound healing in rabbit maxillary sinus mucosaPubMed: 19958244
  • Journal of Clinical PeriodontologyExperimental peri-implant mucositis at different implant surfaces.Pubmed: 24521508
  • Physiological ResearchBudesonide added to modified porcine surfactant Curosurf may additionally improve the lung functions in meconium aspiration syndrome.Pubmed: 2432969
  • Rheumatology InternationalSerum levels of selected chemokines in systemic lupus erythematosus patientsPubmed: 22461186
  • J Med Life.Expression of interleukine-8 as an independent prognostic factor for sporadic colon cancer disseminationPubmed:Pmc4197484
  • J Clin Periodontol.Experimental peri‐implant mucositis at different implant surfacesPubmed:24521508
  • British Journal of NutritionGrape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental?×?Qinchuan steersPubmed:24846452
  • TheriogenologyInflammatory cytokine concentrations in uterine flush and serum samples from dairy cows with clinical or subclinical endometritisTheriojournal:Source
  • Anim Reprod Sci.Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranesPubMed: 25851495
  • Медицина и здравоохранениеДИНАМИКА ЦИТОКИНОВОГО ПРОФИЛЯ В ОЦЕНКЕ ЭФФЕКТИВНОСТИ РАЗНЫХ МЕТОДОВ СТИМУЛЯЦИИ РЕПАРАТИВНОГО ОСТЕОГЕНЕЗА В ЭКСПЕРИМЕНТЕArticle: N
  • BMC AnesthesiologyHyperinflation deteriorates arterial oxygenation and lung injury in a rabbit model of ARDS with repeated open endotracheal suctioningPubMed: 25943099
  • J Breath ResEffects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgeryPubMed: 25719610
  • ARTICLEThe Predictive Diagnostic and Prognostic Cut-off Values for Interleukin 8 in Patients with Meningitis in EgyptProfile: Mohmad_Mosaad
  • Veterinary Immunology and ImmunopathologyNeutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation periodPubmed:27090626
  • Infection, Genetics and EvolutionInfection of chicken bone marrow mononuclear cells with subgroup J avian leukosis virus inhibits dendritic cell differentiation and alters cytokine expressionPubmed:27349993
  • Asian Pacific Journal of Tropical MedicinePerfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injuryscience:S1995764516300955
  • Archives of Biological SciencesDiabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrolpublication:299492265_Diabetes-induced_renal_failu
  • Journal of Agricultural BiotechnologyGln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells10610
  • journal of molecular and cellular cardiologyMutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease.pubmed:28088561
  • Physiological ResearchIntravenous dexamethasone attenuated inflammation and influenced apoptosis of lung cells in an experimental model of acute lung injury.pubmed:28006948
  • PLOS ONEMechanical Ventilation Alters the Development of Staphylococcus aureus Pneumonia in Rabbit.pubmed:27391952
  • The Journal of Comenius University in BratislavaEffects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung Injuryacm.2017.16.issue-3
  • Journal of Physiology and PharmacologyEffects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavagemodel of acute lung injury.pubmed:28195073
  • 中国药物警戒小剂量阿奇霉素对稳定期老年慢性阻塞性肺疾病患者外周血内TNF-α、IL-8、CRP水平及肺功能的影响Periodical_zgywjj201608004
  • Veterinary Immunology and ImmunopathologyCytokine levels in colostrum and in foals' serum pre- and post-sucklingpubmed:28242000

Related products for research use of Homo sapiens (Human) Organism species