ELISA Kit for Matrix Metalloproteinase 10 (MMP10)Homo sapiens (Human)Sandwich ELISA

STMY2; SL2; Stromelysin 2; Transin-2

  • Product No.SEA098Hu
  • Organism SpeciesHomo sapiens (Human)Same name, Different species.
  • Sample TypeSerum, plasma and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range78.12-5000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 34pg/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 10 (MMP10).
    No significant cross-reactivity or interference between Matrix Metalloproteinase 10 (MMP10) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection
  • DownloadInstruction Manual
  • Format48T96T96T*596T*1096T*100
  • FOBUS$ 441US$ 630US$ 2835US$ 5355US$ 44100
  • ELISA Kit for Matrix Metalloproteinase 10 (MMP10)Package and Components
  • ELISA Kit for Matrix Metalloproteinase 10 (MMP10)Package and Components
  • ELISA Kit for Matrix Metalloproteinase 10 (MMP10)Results demonstration
  • SEA098Hu.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Matrix Metalloproteinase 10 (MMP10) and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Metalloproteinase 10 (MMP10) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)92-9995
heparin plasma(n=5)80-10392

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 10 (MMP10) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 10 (MMP10) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Metalloproteinase 10 (MMP10) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)89-97%97-105%91-105%98-105%
heparin plasma(n=5)92-99%81-94%96-104%85-94%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Matrix Metalloproteinase 10 (MMP10)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 10 (MMP10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 10 (MMP10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 10 (MMP10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 10 (MMP10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reference

  • Journal of Cellular and Molecular MedicineType-specific dysregulation of matrix metalloproteinases and their tissue inhibitors in end-stage heart failure patients: relationship between MMP-10 and LV remodellingWiley: source
  • PLoS One.Matrix Metalloproteinase-10 Is Required for Lung Cancer Stem Cell Maintenance, Tumor Initiation and Metastatic PotentialPubMed: PMC3335833
  • PLoS ONEA Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
  • Thromb Haemost.?Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic strokePubmed:24671655
  • Brain, Behavior, and ImmunityPlasma inflammatory biomarkers for Huntington’s disease patients and mouse model Pubmed:25266150
  • Biomedical MaterialsIn vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative studyPubmed:25634132
  • Brain, Behavior, and ImmunityPlasma inflammatory biomarkers for Huntington’s disease patients and mouse modelPubMed: 25266150