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ELISA Kit for Matrix Metalloproteinase 7 (MMP7) Homo sapiens (Human) Sandwich ELISA

MPSL1; MAT; PUMP-1; Matrilysin Uterine; Matrilysin; Matrin; Pump-1 Protease; Uterine Metalloproteinase

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  • ELISA Kit for Matrix Metalloproteinase 7 (MMP7) Packages (Simulation)
  • ELISA Kit for Matrix Metalloproteinase 7 (MMP7) Packages (Simulation)
  • ELISA Kit for Matrix Metalloproteinase 7 (MMP7) Results demonstration
  • SEA102Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Matrix Metalloproteinase 7 (MMP7) and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Metalloproteinase 7 (MMP7) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-104 101
heparin plasma(n=5) 88-102 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 7 (MMP7) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 7 (MMP7) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Metalloproteinase 7 (MMP7) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-103% 84-93% 78-93% 89-97%
heparin plasma(n=5) 92-105% 86-101% 90-98% 97-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Matrix Metalloproteinase 7 (MMP7)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 7 (MMP7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 7 (MMP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 7 (MMP7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 7 (MMP7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • A novel, orally active LPA1 receptor antagonist inhibits lung fibrosis in the mouse bleomycin modelPubMed: 20649573
  • Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis VirusPubmed: source
  • A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
  • Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic strokePubmed:24671655
  • Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cellsPubMed: 26254457
  • Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infectionPubmed:26925446
  • Systematic phenotyping and correlation of biomarkers with lung function and histology in lung fibrosisPubmed:26993522
  • The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cellspubmed:29224019
  • Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult10371/137708
  • Large-scale proteomics identifies MMP-7 as a sentinel of epithelial injury and of biliary atresia.pubmed:29167395
  • Diagnostic Accuracy of Serum Matrix Metalloproteinase‐7 for Biliary AtresiaPubmed: 30153340
  • Quantification of Serum Matrix Metallopeptide 7 Levels May Assist in the Diagnosis and Predict the Outcome for Patients with Biliary AtresiaPubmed: 30853207
  • Serum MMP-7 in the Diagnosis of Biliary AtresiaPubmed: 31604829
  • Human umbilical cord blood mononuclear cells protect against renal tubulointerstitial fibrosis in cisplatin-treated ratsPubmed: 31710895
  • The synthetic toxin biliatresone causes biliary atresia in micePubmed: 32681026
  • Interstitial Score and Concentrations of IL-4R¦Á, PAR-2, and MMP-7 in Bronchoalveolar Lavage Fluid Could Be Useful Markers for Distinguishing Idiopathic Interstitial?¡­33924683
  • A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer34215253

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