ELISA Kit for Myeloperoxidase (MPO) Homo sapiens (Human) Sandwich ELISA

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  • ELISA Kit for Myeloperoxidase (MPO) Packages (Simulation)
  • ELISA Kit for Myeloperoxidase (MPO) Packages (Simulation)
  • ELISA Kit for Myeloperoxidase (MPO) Results demonstration
  • SEA601Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Myeloperoxidase (MPO) and the recovery rates were calculated by comparing the measured value to the expected amount of Myeloperoxidase (MPO) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-102 98
EDTA plasma(n=5) 79-91 85
heparin plasma(n=5) 81-89 84

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloperoxidase (MPO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloperoxidase (MPO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Myeloperoxidase (MPO) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 87-97% 87-104% 88-105%
EDTA plasma(n=5) 90-98% 94-101% 85-104% 91-98%
heparin plasma(n=5) 98-105% 85-97% 93-101% 91-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Myeloperoxidase (MPO)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloperoxidase (MPO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myeloperoxidase (MPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myeloperoxidase (MPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myeloperoxidase (MPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

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  • A New Immunosensor for Serum Myeloperoxidase Based on Self-Assembly of Multi-Walled Carbon Nanotubes/Thionine/Gold Nanoparticles-ChitosanScientific:1207
  • T cells that target carcinoembryonic antigen eradicate orthotopic pancreatic carcinomas without inducing autoimmune colitis in micePubMed: 22750462
  • Rosuvastatin Reloading before Cardiac Surgery with Cardiopulmonary BypassPubMed: 23406839
  • Protective effects of Ankaferd blood stopper on aspirin-induced oxidative mucosal damage in a rat model of gastric injury.PubMed: 23114375
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  • Amentoflavone inhibits iNOS, COX-2 expression and modulates cytokine profile, NF-κB signal transduction pathways in rats with ulcerative colitisPubmed: 24126114
  • Neuroprotective effects of α-ketoglutarate and ethyl pyruvate against motor dysfunction and oxidative changes caused by repeated 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine exposure in micePubmed: 23821591
  • Protective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunitsScienceDirect: S1567576913004153
  • Novel CoQ10 Antidiabetic Mechanisms Underlie Its Positive Effect: Modulation of Insulin and Adiponectine Receptors, Tyrosine Kinase, PI3K, Glucose Transporters, sRAGE and Visfatin in Insulin Resistant/Diabetic RatsPubmed:24586567
  • Heparin-binding protein (HBP/CAP37) - a link to endothelin-1 in endotoxemia-induced pulmonary oedema?Pubmed:24611481
  • Protective Effect of?Acacia ferruginea?against Ulcerative Colitis via Modulating Inflammatory Mediators, Cytokine Profile and NF-κB Signal Transduction PathwaysPubmed:24941292
  • Neuroprotective effects of rosuvastatin against traumatic spinal cord injury in ratsPubmed:25084223
  • Comparison of the Effects of Enoxaparin and Heparin on Inflammatory Biomarkers in Patients with ST-segment Elevated Myocardial Infarction: A prospective Open Label Pilot Clinical TrialPubmed:25237354
  • Gastroprotective effect of nymphayol isolated from Nymphaea stellata (Willd.) flowers: Contribution of antioxidant, anti-inflammatory and anti-apoptotic activitiesPubmed:25289771
  • Melatonin attenuates intestinal ischemia–reperfusion-induced lung injury in rats by upregulating N-myc downstream-regulated gene 2Pubmed:25491174
  • Sulfiredoxin-1 protects primary cultured astrocytes from ischemia-induced damagePubmed:25620665
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  • The Syk Inhibitor Fostamatinib Decreases the Severity of Colonic Mucosal Damage in a Rodent Model of ColitisPubMed: 26116555
  • Preparation of Monoclonal Antibodies and a Simple Myeloperoxidase-Immunosorbent Assay for Detecting Human MyeloperoxidasePubmed:26978734
  • The efficacy of tyrosine kinase inhibitor dasatinib on colonic mucosal damage in murine model of colitisPubmed:26823039
  • THE ANTI-HYPERCHOLESTEROLEMIC EFFECT OF ULVAN POLYSACCHARIDE EXTRACTED FROM THE GREEN ALGA ULVAFASCIATA ON AGED HYPERCHOLESTEROLEMIC RATSjournals:10835
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  • Exploring the Potential of cfDNA Measurements After an Exhaustive Cycle Ergometer Test as a Marker for Performance Related Parameterspubmed:27617485
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  • Sevoflurane Attenuates Ischemia-Reperfusion Injury in a Rat Lung Transplantation Model.pubmed:28190546
  • Fluctuation in the number, type and activity of blood neutrophils in cows exhibiting successful and unsuccessful completion of gestation cycle09291016.2017.1311984
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  • Enzyme catalysis-electrophoresis titration for multiplex enzymatic assay via moving reactionboundary chip.pubmed:27464600
  • Effect of Poly-herbal mixture and butyric acid feeding on immune parameters of postpartum Murrah buffaloespublication:299575009
  • Frequency of Cholesterol Crystals in Culprit Coronary Artery Aspirate During Acute Myocardial Infarction and Their Relation to Inflammation and Myocardial Injurypubmed:28867129
  • Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Techniquepubmed:28408942
  • Effect of photobiomodulation therapy on reducingthe chemo-induced oral mucositis severity and on salivary levels of CXCL8/interleuki8, nitrite, and myeloperoxidase in patients undergoing hematopoietic stem cell transplantation: a randomized clinical trialarticle:10.1007
  • Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescriptionpubmed:28672924
  • Upregulation of Intestinal Barrier Function in Mice with DSS-Induced Colitis by a Defined Bacterial Consortium Is Associated with Expansion of IL-17A Producing Gamma Delta T Cells10.3389:fimmu.2017.00824
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  • Hepatoprotective effect of taurine and coenzyme Q10 and their combination against acrylamide-induced oxidative stress in rats10.4314/tjpr.v16i8.14
  • Role of NOD2 and S100A8/S100A9 in the pathogenesis of Coxsackievirus B3-induced myocarditis10.14279/depositonce-5934
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  • The Plasma Kallikrein–Kininogen Pathway is critical in the Pathogenesis of colitis in MicePubmed:29467753
  • Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal …Pubmed:29375374
  • Evaluation of the anti-hypertensive effect of Tengfu Jiangya tablet by combination of UPLC-Q-exactive-MS-based metabolomics and iTRAQ-based proteomics …Pubmed:29453042
  • Resolvin D1 promotes corneal epithelial wound healing and restoration of mechanical sensation in diabetic micePubmed:29643724
  • Effect of tropical thermal stress on peri-implantation immune responses in cowsPubmed:29625402
  • Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in ratsPubmed:29956781
  • Hydrogen‑rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus‑activated kinase 2/signal transducer and activator of …Pubmed:29928398
  • Associations of periparturient plasma biochemical parameters, endometrial leukocyte esterase and myeloperoxidase, and bacterial detection with clinical and …Pubmed:29332864
  • Sitagliptin attenuates intestinal ischemia/reperfusion injury via cAMP/PKA, PI3K/Akt pathway in a glucagon-like peptide 1 receptor-dependent mannerPubmed: 30195035
  • Effect of β-glucan on acetic acid-induced colitis in rats
  • Chlorogenic Acid Attenuates Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice through MAPK/ERK/JNK Pathway
  • Bacillus licheniformis Zhengchangsheng® attenuates DSS-induced colitis and modulates the gut microbiota in mice
  • Do Late Phase Hyperbaric and Normobaric Oxygen Therapies have Effect on Liver Damage?: An Experimental Sepsis Model
  • Posttreatment With LYRM03 Protects Rats From Acute Lung Inflammation Induced by Lipopolysaccharide via Suppressing the NF-κB/MyD88/TLR4 AxisPubmed: 31255931
  • Efficacy assessment of co-treated alpha-ketoglutarate and N-acetyl cysteine against the subchronic toxicity of cyanide in ratsPubmed: 31244408
  • The effects of hydrogen-rich saline solution on intestinal anastomosis performed after intestinal ischemia reperfusion injury
  • Annexin A1 mimetic peptide Ac2-26 attenuates mechanical injury induced corneal scarring and inflammationPubmed: 31519322
  • SHP2 deficiency promotes Staphylococcus aureus pneumonia following influenza infectionPubmed: 31782850
  • Effects of ozone preconditioning on recovery of rat colon anastomosis after preoperative radiotherapyPubmed: 31895975
  • Effects of a high concentration of hydrogen on neurological function after traumatic brain injury in diabetic ratsPubmed: 31926128
  • Native myeloperoxidase is required to make the experimental vasculitis modelPubmed: 31864385
  • Rational Modulation of the Luminescence of Upconversion Nanomaterials with Phycocyanin for the Sensing and Imaging of Myeloperoxidase during an Inflammatory …Pubmed: 32126753
  • Peripartum changes in the activity and expression of neutrophils may predispose to the postpartum occurrence of metritis in dairy cowsPubmed: 33229058
  • The HDL from septic-ARDS patients with composition changes exacerbates pulmonary endothelial dysfunction and acute lung injury induced by cecal ligation …Pubmed: 33148285
  • Lead exposure induces cell autophagy via blocking the Akt/mTOR signaling in rat astrocytesPubmed: 32879255
  • A comparative study on various immunological parameters influencing embryo survivability in crossbred dairy cowsPubmed: 32810791
  • Sensitive and rapid lateral-flow assay for early detection of subclinical mammary infection in dairy cowsPubmed: 32636462
  • Gambogic acid alleviates inflammation and apoptosis and protects the blood-milk barrier in mastitis induced by LPSPubmed: 32585608
  • Protective Effects of SIRT6 Overexpression against DSS-Induced Colitis in MicePubmed: 32580272
  • DNA microarray analysis of hypothermia-exposed murine lungs for identification of forensic biomarkersPubmed: 33161360
  • Juglanin administration protects skin against UVB‑induced injury by reducing Nrf2‑dependent ROS generationPubmed: 32377697
  • The adverse alterations of HDL in quality promote septic ARDS via exacerbated pulmonary endothelial dysfunction
  • Ameliorating Effects of ¦Â-glucan on Epigastric Artery Island Flap Ischemia-Reperfusion Injury33477077
  • Dental pulp stem cells overexpressing hepatocyte growth factor facilitate the repair of DSS-induced ulcerative colitis33413675
  • Inhibition of nitric oxide synthase aggravates brain injury in diabetic rats with traumatic brain injury33433486
  • Strictosamide alleviates the inflammation in an acute ulcerative colitis (UC) model33595775
  • Protective effects of hydrogen rich saline solution on ventral penile Mathieu type flap with penile tourniquet application in rats33608226
  • JAK3 and PI3K mediates the suppressive effects of interferon tau on neutrophil extracellular traps formation during peri-implantation period33862433
  • Macrophage Extracellular Traps Aggravate Iron Overload©\Related Liver Ischemia/Reperfusion Injury33959955
  • Characterization of a Rat Model of Myeloperoxidase-Anti-Neutrophil Cytoplasmic Antibody-Associated Crescentic Glomerulonephritis33910203
  • Topical calcitriol application promotes diabetic corneal wound healing and reinnervation through inhibiting NLRP3 inflammasome activation34144035
  • Daphnetin ameliorates acute lung injury in mice with severe acute pancreatitis by inhibiting the JAK2¨CSTAT3 pathway34075160
  • ¦Ê?opioid receptor agonist U50488H attenuates postoperative cognitive dysfunction of cardiopulmonary bypass rats through the PI3K/AKT/Nrf2/HO?1 pathway33649775
  • Novel 1, 3, 4-Oxadiazole Derivatives of Pyrrolo [3, 4-d] Pyridazinone Exert Anti-Inflammatory Activity without Acute Gastrotoxicity in the Carrageenan-Induced …34754217
  • Calycosin attenuates severe acute pancreatitis-associated acute lung injury by curtailing high mobility group box 1-induced inflammation34908806
  • Blocking tumor necrosis factor-α delays progression of chronic obstructive pulmonary disease in rats through inhibiting MAPK signaling pathway and activating …34630665
  • Hyperactive neutrophils infiltrate vital organs of tumor bearing host and contribute to gradual systemic deterioration with tumor progression34890699
  • Saccharomyces cerevisiae fermentation products reduce bacterial endotoxin concentrations and inflammation during grain-based subacute ruminal acidosis …Pubmed:34998547
  • Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot StudyPubmed:35743980
  • Serum Myeloperoxidase as A Biomarker of Asthma Severity Among Adults: A Case Control StudyPubmed:35765520
  • Bovine lactoferricin ameliorates intestinal inflammation and mucosal barrier lesions in colitis through NF-κB/NLRP3 signaling pathways
  • A Novel Role of Tinospora Cordifolia in Amelioration of Cancer-Induced Systemic Deterioration By Taming Neutrophil Infiltration and Hyperactivation

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