ELISA Kit for Nitric Oxide Synthase 1, Neuronal (NOS1) Homo sapiens (Human) Sandwich ELISA

NOS; nNOS; IHPS1; NC-NOS; N-NOS; bNOS; Neuronal NOS; Constitutive NOS; NOS type I; Nitric Ocide Synthase; Peptidyl-cysteine S-nitrosylase NOS1

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • ELISA Kit for Nitric Oxide Synthase 1, Neuronal (NOS1) Packages (Simulation)
  • ELISA Kit for Nitric Oxide Synthase 1, Neuronal (NOS1) Packages (Simulation)
  • ELISA Kit for Nitric Oxide Synthase 1, Neuronal (NOS1) Results demonstration
  • SEA815Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Nitric Oxide Synthase 1, Neuronal (NOS1) and the recovery rates were calculated by comparing the measured value to the expected amount of Nitric Oxide Synthase 1, Neuronal (NOS1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-104 101
EDTA plasma(n=5) 85-99 93
heparin plasma(n=5) 88-103 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nitric Oxide Synthase 1, Neuronal (NOS1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nitric Oxide Synthase 1, Neuronal (NOS1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nitric Oxide Synthase 1, Neuronal (NOS1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-103% 83-99% 96-103% 82-91%
EDTA plasma(n=5) 89-104% 78-105% 97-104% 83-91%
heparin plasma(n=5) 91-104% 90-104% 95-103% 94-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Nitric Oxide Synthase 1, Neuronal (NOS1)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase 1, Neuronal (NOS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nitric Oxide Synthase 1, Neuronal (NOS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nitric Oxide Synthase 1, Neuronal (NOS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nitric Oxide Synthase 1, Neuronal (NOS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric OxidePubMed: 23359814
  • The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disordersPubmed:25245337
  • Состояние эндотелиально-сосудистого фактора у беременных с артериальной гипертензиейhandle:123
  • Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removalPubmed:29858659
  • Antioxydant and Endotheliotropic Properties 4-Hydroxy-3, 5-Di-Tret-Butyl Cinnamic Acid under Conditions of Experimental Brain Ischemia:
  • Tadalafil Preserves Penile Nitric Oxide Synthase from Detrimental Effect of Paroxetine in RatsDoi: 10.5152/eurasianjmed.2018.18160
  • The Role of Reactive Oxygen Species and Nitric Oxide in the Inhibition of Trichophyton rubrum Growth by HaCaT CellsPubmed: 32104540
  • Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial …Pubmed: 31734543
  • Protective Effects of Rocuronium Bromide on Ischemia-Reperfusion Injury in Skeletal Muscle Induced by Tourniquet in Patients Undergoing Elective Unilateral Total …Pubmed: 32884241
  • Evaluation of salivary and serum methylated arginine metabolites and nitric oxide synthase in advanced periodontitis patientsPubmed:35426000

Recommend products