ELISA Kit for Osteocalcin (OC) Homo sapiens (Human) Sandwich ELISA

BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • ELISA Kit for Osteocalcin (OC) Packages (Simulation)
  • ELISA Kit for Osteocalcin (OC) Packages (Simulation)
  • ELISA Kit for Osteocalcin (OC) Results demonstration
  • SEA471Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Osteocalcin (OC) and the recovery rates were calculated by comparing the measured value to the expected amount of Osteocalcin (OC) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-98 94
EDTA plasma(n=5) 90-98 93
heparin plasma(n=5) 80-90 82

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteocalcin (OC) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteocalcin (OC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Osteocalcin (OC) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-93% 92-99% 84-98% 94-105%
EDTA plasma(n=5) 79-97% 85-95% 80-97% 80-102%
heparin plasma(n=5) 80-103% 92-101% 79-88% 79-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Osteocalcin (OC)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteocalcin (OC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteocalcin (OC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteocalcin (OC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Osteocalcin (OC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Surface engineering of titanium with potassium hydroxide and its effects on the growth behavior of mesenchymal stem cellsPubMed: 19963080
  • Biochemical study of the influence of ad type biophytomodulators in healing of bone defects in rats.Analelesnbc: source
  • Variability of Osteocalcin Status in Chinese Holstein Cattle: Do Phylogeny, Vitamin D or Gene Polymorphisms Matter?Ajas: source
  • Dyslipidemic high-fat diet affects adversely bone metabolism in mice associated with impaired antioxidant capacityScienceDirect: S0899900709004717
  • Osteogenic activity of silymarin through enhancement of alkaline phosphatase and osteocalcin in osteoblasts and tibia-fractured miceRsm: source
  • Oxytocin promotes bone formation during the alveolar healing process in old acyclic female ratsScienceDirect: S0003996912001021
  • Regulation of the differentiation of mesenchymal stem cells in vitro and osteogenesis in vivo by microenvironmental modification of titanium alloy surfacesScienceDirect: S0142961212000865
  • Exendin-4 Increases Bone Mineral Density in Type 2 Diabetic OLETF Rats Potentially Through the Down-Regulation of SOST/Sclerostin in OsteocytesPubMed: 23357248
  • Serum changes induced by intramedullar experimental administration of bisphosphonatesPubMed: 21424089
  • Valvular osteoclasts in calcification and aortic valve stenosis severityPubMed: 23452891
  • Postnatal administration of 2-oxoglutaric acid improves articular and growth plate cartilages and bone tissue morphology in pigs prenatally treated with dexamethasonePubMed: 23211309
  • Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in miceWiley: Source
  • Puerarin promotes osteogenesis and inhibits adipogenesis in vitroPubMed: PMC3765709
  • Magnesium prevents phosphate-induced calcification in human aortic vascular smooth muscle cellsPubMed: PMC3611891
  • Morphological changes of the cartilage and bone in newborn piglets evoked by experimentally induced glucocorticoid excess during pregnancyPubmed: 22716040
  • The use of Tri-Calcium Phosphate (TCP) and stem cells for the regeneration of osteoperiosteal critical-size mandibular bony defects, an in vitro and preclinical studyPubmed: 24485270
  • Effect of the “protein diet” and bone tissue.Pubmed: 24483972
  • Valvular osteoclasts in calcification and aortic valve stenosis severity.Pubmed: 23452891
  • Effects and mechanisms of 8-prenylnaringenin on osteoblast MC3T3-E1 and osteoclast-like cells RAW264.7Onlinelibrary: fsn3.109
  • Effect of the “protein diet” and bone tissueAulamedica: Source
  • Toward the Development and Characterization of an Easy Handling Sheet-Like Biohybrid Bone SubstitutePubmed:25761235
  • Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory ConditionsWiley:Source
  • Effects and mechanisms of 8‐prenylnaringenin on osteoblast MC3T3‐E1 and osteoclast‐like cells RAW264. 7Pubmed:Pmc4221832
  • The use of TriCalcium Phosphate (TCP) and stem cells for the regeneration of osteoperiosteal critical-size mandibular bony defects, an in vitro and preclinical studyPubmed:24485270
  • Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of ratsPubmed:25038619
  • Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cellsPubmed:25542352
  • Pathophysiological mechanism of bone loss in type 2 diabetes involves inverse regulation of osteoblast function by PPARγ coactivator-1α and skeletal muscle atrogenes: adiponectin receptor 1 as a potential target for reversing diabetes-induced osteopeniaPubmed:25633418
  • Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiationPubmed:25678785
  • Transplantation of mesenchymal stem cells, recombinant human BMP-2,and their combination in accelerating the union after osteotomy and increasing, the mechanical strength of extracorporeally irradiated femoral autograft in rat modelsPubmed:25679008
  • Inhibition of osteoclast activation by phloretin through disturbing αvβ3 integrin-c-Src pathwayPubmed:25834823
  • The influence of bexarotene, a selective agonist of the retinoid receptor X (RXR), and tazarotene, a selective agonist of the retinoid acid receptor (RAR), on bone metabolism in ratsPubMed: 26569440
  • Biological Evaluation of a Prototype Material made of Polyglycolic Acid and HydroxyapatiteArticle: Jhtb
  • Products of dentin matrix protein-1 degradation by interleukin-1β-induced matrix metalloproteinase-3 promote proliferation of odontoblastic cellsPubMed: 26355224
  • Body fat loss induced by calcium in co-supplementation with conjugated linoleic acid is associated with increased expression of bone formation genes in adult micePubMed: 26454511
  • Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathwayPubMed: 26677102
  • Antiosteoporotic effect of icariin in ovariectomized rats is mediated via the Wnt/β-catenin pathwayPubmed:27347050
  • Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Ratsprofile:303311214
  • Enhanced biocompatible and hemocompatible nano/micro porous surface as a biological scaffold for functionalizational and biointegrated implantsscience:S0045653516302016
  • The effects of strength training and raloxifene on bone health in aging ovariectomized ratsPubmed:26812611
  • Effect of Sodium Fluoride on Bone Biomechanical and Histomorphometric Parameters and on Insulin Signalingand Insulin Sensitivity in Ovariectomized Rats.Pubmed:26876375
  • Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87
  • Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickensview:87
  • MiR-142-5p promotes bone repair by maintaining osteoblast activityPubmed:27085967
  • Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats:
  • Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight womenS1756464617301561
  • Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation.pubmed:28272338
  • The controlled release of simvastatin from TiO2 nanotubes to promote osteoblast differentiation and inhibit osteoclast resorptionS0169433216326861
  • Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats.pubmed:27908549
  • Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathwaypubmed:27387537
  • Fast and long acting neoflavonoids dalbergin isolated from Dalbergia sissoo heartwood is osteoprotective in ovariectomized model of osteoporosis: Osteoprotective …S0753332216308484
  • Serum N-terminal telopeptide of type I collagen as an early marker of fracture nonunion in rabbits.pubmed:28105092
  • Effects of long-term administration of pantoprazole on bone mineral density in young male rats.pubmed:27552060
  • N-terminal telopeptides of type I collagen and bone mineral density for early diagnosis ofnonunion: An experimental study in rabbits.pubmed:27512225
  • Micromolar Levels of Sodium Fluoride Promote Osteoblast Differentiation Through Runx2 Signalingpubmed:28083749
  • Effects of fluoride on insulin signaling and bone metabolism in ovariectomized rats.pubmed:27908407
  • Synthesis of polypyrrole nanowires with positive effect on MC3T3-E1 cell functions through electrical stimulation.pubmed:27987727
  • Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized ratsS0753332216322752
  • Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Dietarticle:10.1007
  • Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken10.159:1806-9061-2016-0285
  • Oral administration of kaempferol inhibits bone loss in rat model of ovariectomy-induced osteopeniapubmed:29031689
  • MiR‑142‑5p promotes bone repair by maintaining osteoblast activitypubmed:27085967
  • A novel osteoporosis model with ascorbic acid deficiency in Akr1A1 gene knockout mice.pubmed:28060768
  • Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine. pubmed:29049953
  • Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation.pubmed:28742995
  • A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/β-catenin Signaling Pathways in Ovariectomized RatsISSN 2249 – 622X
  • Stabilization of Ca-deficient hydroxyapatite in biphasic calcium phosphate ceramics by adding alginate to enhance their biological performances10.1039/C7TB02620J 
  • Osteogenic commitment and differentiation of human mesenchymal stem cells by low-intensity pulsed ultrasound stimulation.pubmed:28621452
  • BMP-2 gene transfection of bone marrow stromal cells to induce osteoblastic differentiation in a rat calvarial defect modelPubmed:30033316
  • Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats10.1016:j.jff.2018.04.048
  • Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat modelPubmed:29705515
  • Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathwayPubmed:29883721
  • Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickensPubmed:29373588
  • Stabilization of Ca-deficient hydroxyapatite in biphasic calcium phosphate ceramics by adding alginate to enhance their biological performancesc7tb02620j
  • Effects of constrained dynamic loading, CKIP‑1 gene knockout and combination stimulations on bone loss caused by mechanical unloadingPubmed:29956799
  • A Comparison of 1‐and 3.2‐MHz Low‐Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo StudyPubmed:29781183
  • Dietary supplementation for Santa Inês hair ewes on pasture at pre-and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolismPubmed:29931604
  • Effects of KLD-12/Recombinant Human Bone Morphogenetic Protein-2 Nanofiber Scaffold on the Osteogenic Activity of Rabbit Bone Marrow Mesenchymal Stem Cells10.1166:jbt.2018.1719
  • Enhanced osteoinductivity of porous biphasic calcium phosphate ceramic beads with high content of strontium incorporated calcium deficient hydroxyapatiteDoi: 10.1039/C8TB01554F
  • Combined effect of pulsed electromagnetic fields and narrowband ultraviolet B on bone metabolism in glucocorticoid-treated rats
  • Beta-Cyclodextrin Modified Mesoporous Bioactive Glass Nanoparticles/Silk Fibroin Hybrid Nanofibers as an Implantable Estradiol Delivery System for the Potential …Pubmed: 30255905
  • In Situ Self-Assembly of Graphene Oxide/Polydopamine/Sr2+ Nanosheets on Titanium Surfaces for Enhanced Osteogenic Differentiation of Mesenchymal Stem CellsDoi: 10.1016/j.carbon.2018.10.081
  • The decline of whole-body glucose metabolism in ovariectomized ratsPubmed: 30292771
  • Mollugin attenuates glucocorticoid-induced osteoporosis in rats via Akt/P13K pathway
  • Transcriptome analysis of osteoblasts in an ovariectomized mouse model in response to physical exercise
  • NUMB maintains bone mass by promoting degradation of PTEN and GLI1 via ubiquitination in osteoblastsPubmed: 30455992
  • Osteoinductivity of porous biphasic calcium phosphate ceramic spheres with nanocrystalline and their efficacy in guiding bone regenerationPubmed: 30629405
  • CoCr porous scaffolds manufactured via selective laser melting in orthopedics: Topographical, mechanical, and biological characterizationPubmed: 30689288
  • Lead exposure inhibits osteoblastic differentiation and inactivates the canonical Wnt signal and recovery by icaritin in MC3T3-E1 subclone 14 cellsPubmed: 30731080
  • Effect and mechanism of psoralidin on promoting osteogenesis and inhibiting adipogenesisDoi: 10.1016/j.phymed.2019.152860
  • Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and OsteoblastsPubmed: 30838809
  • Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9
  • Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis
  • Influence of physical training on markers of bone turnover, mechanical properties, morphological alterations, density and mineral contents in the femur of rats exposed …Pubmed: 30983556
  • Methylbenzoxime as a therapeutic agent for glucocorticoid-induced osteoporosis in rats.
  • Silent Mating Type Information Regulation 2 Homolog (SIRT1) Influences Osteogenic Proliferation and Differentiation of MC3T3-E1 Cells via Regulation of miR-132 …Pubmed: 30923307
  • Alpha ketoglutarate exerts a pro-osteogenic effect in osteoblast cell lines through activation of JNK and mTOR/S6K1/S6 signaling pathwaysPubmed: 31051157
  • Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and …
  • A dual functional bone‐defect‐filling material with sequential antibacterial and osteoinductive properties for infected bone defect repairPubmed: 31173657
  • Use of Polyphenol Tannic Acid to Functionalize Titanium with Strontium for Enhancement of Osteoblast Differentiation and Reduction of Osteoclast ActivityPubmed: 31362449
  • Laboratory Monitoring of Bone Tissue Remodeling after Augmentation of Impression Intraarticular Fracture with Different Types of Bone GraftPubmed: 31630302
  • Nano-Hydroxyapatite Coating Promotes Porous Calcium Phosphate Ceramic-Induced Osteogenesis Via BMP/Smad Signaling PathwayPubmed: 31632013
  • AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activitiesPubmed: 31739209
  • Alleviating Effect of α-Lipoic Acid and Magnesium on Cadmium-Induced Inflammatory Processes, Oxidative Stress and Bone Metabolism Disorders in Wistar RatsPubmed: 31739465
  • MiR-296 Promotes Osteoblast Differentiation by Upregulating CbfalPubmed: 31694033
  • Beneficial effects of Cuscuta chinensis extract on glucocorticoid-induced osteoporosis through modulation of RANKL/OPG signalsPubmed: 31826180
  • Vinpocetine inhibits RANKL-induced osteoclastogenesis and attenuates ovariectomy-induced bone lossPubmed: 31846839
  • BMP9 reduces bone loss in ovariectomized mice by dual regulation of bone remodelingPubmed: 31914211
  • EDTA-Modified 17β-Estradiol-Laden Upconversion Nanocomposite for Bone-Targeted Hormone Replacement Therapy for OsteoporosisPubmed: 32194868
  • Rutin Improves Bone Histomorphometric Values by Reduction of Osteoclastic Activity in Osteoporosis Mouse Model Induced by Bilateral OvariectomyPubmed: 32172552
  • Positive role of calcium phosphate ceramics regulated inflammation in the osteogenic differentiation of mesenchymal stem cellsPubmed: 32064734
  • HSP90 inhibitors strengthen extracellular ATP‐stimulated synthesis of interleukin‐6 in osteoblasts: Amplification of p38 MAP kinasePubmed: 32567086
  • QKI deficiency leads to osteoporosis by promoting RANKL-induced osteoclastogenesis and disrupting bone metabolismPubmed: 32382069
  • Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanismPubmed: 33000198
  • Comparative study of DHA-enriched phosphatidylcholine and EPA-enriched phosphatidylcholine on ameliorating high bone turnover via regulation of the …Pubmed: 33140795
  • A Hierarchical Janus Nanofibrous Membrane Combining Direct Osteogenesis and Osteoimmunomodulatory Functions for Advanced Bone Regeneration
  • Research on the Mechanism of miR-142-5p's Promotion of the Function and Mineralization of Osteoblasts by Down-regulating the Expression of WWP1 Gene
  • 淫羊藿水提物及提取药渣的抗骨质疏松药效比较研究
  • Bioinspired polydopamine/graphene oxide/collagen nanofilms as a controlled release carrier of bioactive substances
  • In vitro comparison of titanium surface conditioning via boron-compounds and sand-blasting acid-etching
  • Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/¦Â©\catenin pathway33950533
  • Studies of osteoblast-like MG-63 cellular proliferation and differentiation with cyclic stretching cell culture system on biomimetic hydrophilic layers modified polydimethylsiloxane substrate
  • Andrographolide prevents bone loss via targeting ERRalpha‐regulated metabolic adaption of osteoclastogenesis34233019
  • Effects of icariin on the fracture healing in young and old rats and its mechanism34511043
  • Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway34512780
  • Spatiotemporal Management of the Osteoimmunomodulation of Fibrous Scaffolds by Loading a Novel Amphiphilic NanomedicinePubmed:35311248
  • Notoginsenoside R1 Promotes Migration, Adhesin, Spreading, and Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stromal CellsPubmed:35684342
  • Effects of 8-week High-Intensity Interval Training and Moderate-Intensity Continuous Training on Bone Metabolism in Sedentary Young FemalesPubmed:35096081
  • Effect of Antibacterial Enoxacin on the Properties of Injectable Nano-hydroxyapatite/Polyurethane Cement for Bone Repairing

Recommend products