ELISA Kit for Resistin (RETN) Homo sapiens (Human) Sandwich ELISA

ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3

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  • ELISA Kit for Resistin (RETN) Packages (Simulation)
  • ELISA Kit for Resistin (RETN) Packages (Simulation)
  • ELISA Kit for Resistin (RETN) Results demonstration
  • SEA847Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Resistin (RETN) and the recovery rates were calculated by comparing the measured value to the expected amount of Resistin (RETN) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-104 101
EDTA plasma(n=5) 96-105 102
heparin plasma(n=5) 91-101 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Resistin (RETN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Resistin (RETN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Resistin (RETN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-99% 81-104% 86-97% 83-101%
EDTA plasma(n=5) 79-95% 94-103% 81-103% 91-98%
heparin plasma(n=5) 79-96% 87-98% 86-94% 84-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Resistin (RETN)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Resistin (RETN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Resistin (RETN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Resistin (RETN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Resistin (RETN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat dietPubMed: 20869961
  • Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar RatsPubmed:Pmc4363645
  • Estradiol stimulates mitochondrial biogenesis and adiponectin expression in skeletal musclePubmed:24681828
  • Adipose tissue regulates hepatic cholesterol metabolism via adiponectinPubmed:25445438
  • Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty RatsPubmed:25697126
  • Tinospora crispaPubMed: 25821506
  • Longitudinal changes in adipose tissue of dairy cows from late pregnancy to lactation. Part 1: The adipokines apelin and resistin and their relationship to receptors linked with lipolysisPubMed: 26686707
  • Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 3 Diabetic Patients With and Without ComplicationsPubMed: 25120108
  • Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokinesscience:S0065128116301234
  • Endocrine activity of adipose tissues as influenced by energy intake in the periparturient cow108495429X:34
  • Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats.pubmed:28246535
  • FAM3A enhances adipogenesis of 3T3-L1 preadipocytes via activation of ATP-P2 receptor-Akt signaling pathwaypubmed:28515350
  • Involvement of plasma adipokines in metabolic and reproductive parameters in Holstein dairy cows fed with diets with differing energy levelspubmed:28803009
  • Biological effects of adipocytes in sulfur mustard induced toxicitypubmed:29129815
  • Bashar Zeidan1, Antigoni Manousopoulou2, Diana J. Garay-Baquero2, 3, Cory H. White3, 7, Samantha ET Larkin1, Kathleen N. Potter1, Theodoros I …Pubmed:29566726
  • Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal ratsPubmed:29784871
  • Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1 …Pubmed:29484120
  • Mango leaf tea promotes hepatoprotective effects in obese ratsDoi: 10.1016/j.jff.2018.09.010
  • Bacupari (Garcinia brasiliensis) extract modulates intestinal microbiota and reduces oxidative stress and inflammation in obese ratsDoi: 10.1016/j.foodres.2019.04.012
  • Extruded sorghum (Sorghum bicolor L.) improves gut microbiota, reduces inflammation, and oxidative stress in obese rats fed a high-fat diet
  • Antidiabetic and cardiovascular beneficial effects of a liver-localized mitochondrial uncouplerPubmed: 31092829
  • Hypothalamic–Pituitary and Adipose Tissue Responses to the Effect of Resistin in Sheep: The Integration of Leptin and Resistin Signaling Involving a Suppressor of …Pubmed: 31514318
  • Saliva resistin as a screening marker of gestational diabetes mellitusPubmed: 32804001
  • Tao-Hong-Si-Wu Decoction promotes angiogenesis after cerebral ischaemia in rats via platelet microparticlesPubmed: 32768169
  • Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body …Pubmed: 32545900
  • Seasonal and Nutritional Fluctuations in the mRNA Levels of the Short Form of the Leptin Receptor (LRa) in the Hypothalamus and Anterior Pituitary in Resistin …34438908
  • High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients
  • Skeletal Muscle Fatty Acid Metabolism is Diverted Towards the Synthesis of Signaling Intermediates Without Fatty Acid Uptake Contribution in Dry Cows with Obesity …

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