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ELISA Kit for Stromal Cell Derived Factor 1 (SDF1) Homo sapiens (Human) Sandwich ELISA

CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12

  • Product No.SEA122Hu
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range0.156-10ng/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 0.057ng/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Stromal Cell Derived Factor 1 (SDF1).No significant cross-reactivity or interference between Stromal Cell Derived Factor 1 (SDF1) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
  • DownloadInstruction Manual
  • UOM 48T96T 96T*5 96T*10 96T*100 In stock
  • FOB US$ 307.00 US$ 438.00 US$ 1,971.00 US$ 3,723.00 US$ 30,660.00
  • Quantity
  • Add to Cart Distributors
  • ELISA Kit for Stromal Cell Derived Factor 1 (SDF1) Packages (Simulation)
  • ELISA Kit for Stromal Cell Derived Factor 1 (SDF1) Packages (Simulation)
  • ELISA Kit for Stromal Cell Derived Factor 1 (SDF1) Results demonstration
  • SEA122Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Stromal Cell Derived Factor 1 (SDF1) and the recovery rates were calculated by comparing the measured value to the expected amount of Stromal Cell Derived Factor 1 (SDF1) in samples.

Matrix Recovery range (%) Average(%)
sodium citrate plasma(n=5) 83-92 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Stromal Cell Derived Factor 1 (SDF1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
sodium citrate plasma(n=5) 85-96% 80-103% 97-105% 80-90%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Stromal Cell Derived Factor 1 (SDF1)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stromal Cell Derived Factor 1 (SDF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stromal Cell Derived Factor 1 (SDF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stromal Cell Derived Factor 1 (SDF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stromal Cell Derived Factor 1 (SDF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Critical CareEndothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD)Goescholar: source
  • BiomaterialsThe promotion of endothelial progenitor cells recruitment by nerve growth factors in tissue-engineered blood vesselsScienceDirect: S0142961209012654
  • BiomaterialsThe use of BDNF to enhance the patency rate of small-diameter tissue-engineered blood vessels through stem cell homing mechanismsScienceDirect: S0142961211011422
  • PhytomedicineTanshinone IIA increases recruitment of bone marrow mesenchymal stem cells to infarct region via up-regulating stromal cell-derived factor-1/CXC chemokine receptor 4 axis in a myocardial ischemia modelScienceDirect: S0944711310003260
  • Graefe's Archive for Clinical and Experimental OphthalmologyMonocyte/macrophages promote vasculogenesis in choroidal neovascularization in mice by stimulating SDF-1 expression in RPE cellsPubMed: 21655971
  • Molecular TherapyPleiotropic beneficial effects of sonic hedgehog gene therapy in an experimental model of peripheral limb ischemiaPubMed: 21224834
  • OncogeneElevated PLGF contributes to small-cell lung cancer brain metastasisPubMed: 22797069
  • International Journal of Nephrology Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with PolyangiitisPubMed: 22792461
  • Rheumatology InternationalSerum levels of selected chemokines in systemic lupus erythematosus patientsPubmed: 22461186
  • Experimental Eye ResearchHyperglycemia promotes vasculogenesis in choroidal neovascularization in diabetic mice by stimulating VEGF and SDF-1 expression in retinal pigment epithelial cellsPubmed: 24780853
  • AtherosclerosisThe non-alcoholic fraction of beer increases stromal cell derived factor 1 and the number of circulating endothelial progenitor cells in high cardiovascular risk subjects: A randomized clinical trialPubmed:24530788
  • International Journal of CardiologyHypoxic exercise training improves cardiac/muscular hemodynamics and is associated with modulated circulating progenitor cells in sedentary menPubmed:24286591
  • Tissue Engineering Part ATissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model RatPubmed:24814095
  • Pharmacol Rep.?Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depressionPubmed:25150002
  • Journal of Stroke and Cerebrovascular DiseasesCirculating CD133+ CD34+ Progenitor Cells and Plasma Stromal-Derived Factor-1Alpha: Predictive Role in Ischemic Stroke PatientsPubmed:25444027
  • Pharmacological ReportsThe MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disordersPubmed:25560580
  • In Vitro Cellular &amp; Developmental Biology - AnimalStromal cell-derived factor 1 (SDF-1) accelerated skin wound healing by promoting the migration and proliferation of epidermal stem cellsPubmed:25636237
  • Pharmacological ReportsSerum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorderPubMed: 26481549
  • Biochem Biophys Res CommunStromal cell-derived factor 1 promoted migration of adipose-derived stem cells to the wounded area in traumatic ratsPubMed: 26392311
  • Am J Transl ResRole of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive ratsPubMed: 26396666
  • Medicine (Baltimore)A Novel Combination of Calprotectin and CXCL13 for Predicting Malignancy in Patients with Exudative Pleural EffusionPubMed: 26632727
  • Pharmacological ReportsA study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidityPubmed:27208878
  • Frontiers in PsychiatryPlasma Chemokines in Patients with Alcohol Use Disorders: Association of CCL11 (Eotaxin-1) with Psychiatric Comorbidity.pubmed:28149283
  • European Journal of Applied PhysiologyHigh-intensity Interval training enhances mobilization/functionality of endothelial progenitor cellsand depressed shedding of vascular endothelial cells undergoing hypoxia.pubmed:27761657

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