ELISA Kit for Transthyretin (TTR)Homo sapiens (Human)Sandwich ELISA

TBPA; PA; PLB; ATTR; PALB; Prealbumin; Prealbumin Amyloidosis Type I; Transports Thyroxine and Retinol

  • Product No.SEA726Hu
  • Organism SpeciesHomo sapiens (Human)Same name, Different species.
  • Sample Typeserum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range0.312-20ng/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 0.151ng/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Transthyretin (TTR).
    No significant cross-reactivity or interference between Transthyretin (TTR) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection
  • DownloadInstruction Manual
  • Format48T96T96T*596T*1096T*100
  • FOBUS$ 441US$ 630US$ 2835US$ 5355US$ 44100
  • ELISA Kit for Transthyretin (TTR)Package and Components
  • ELISA Kit for Transthyretin (TTR)Package and Components
  • ELISA Kit for Transthyretin (TTR)Results demonstration
  • SEA726Hu.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Transthyretin (TTR) and the recovery rates were calculated by comparing the measured value to the expected amount of Transthyretin (TTR) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)93-10198
EDTA plasma(n=5)84-9187
heparin plasma(n=5)82-9586

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transthyretin (TTR) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transthyretin (TTR) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transthyretin (TTR) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)99-105%81-101%78-105%80-92%
EDTA plasma(n=5)93-101%80-105%87-98%96-103%
heparin plasma(n=5)93-105%79-99%85-98%93-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Transthyretin (TTR)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transthyretin (TTR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transthyretin (TTR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transthyretin (TTR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transthyretin (TTR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reference

  • Chinese Medical JournalScreening disease-associated proteins from sera of patients with rheumatoid arthritis: a comparative proteomic studyPubMed: 20367977
  • Journal of Vascular SurgeryCirculation levels of acute phase proteins in patients with Takayasu arteritisPubMed: 20100644
  • Journal of Pharmaceutical and Biomedical AnalysisEffect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2PubMed: 21130593
  • Toxicology LettersSerum proteomic analysis reveals potential serum biomarkers for occupational medicamentosa-like dermatitis caused by trichloroethylenePubmed:24960064
  • Journal of NeurochemistrySchwann cells contribute to neurodegeneration in transthyretin amyloidosisPubmed:25693163
  • Anal BiochemSurface plasmon resonance assay of inhibition by pharmaceuticals for thyroxine hormone binging to transport proteinsPubMed: 26384643