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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
ELISA Kit for Keratin 6A (KRT6A)
K6A; KRT6C; KRT6D; CK6C; K6C; CK6D; K6D; Keratin 6C; Keratin 6D
Organism species Homo sapiens (Human)
Product No.SED234Hu
Sample typeSerum, plasma and other biological fluids.
Format96-well strip plate
Assay length4.5 hours
Detection range15.625-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.625pg/mL
SensitivityThe minimum detectable dose of this kit is typically less than 5.7pg/mL.
Specificity
This assay has high sensitivity and excellent specificity for detection of Keratin 6A (KRT6A).
No significant cross-reactivity or interference between Keratin 6A (KRT6A) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Keratin 6A (KRT6A) and the recovery rates were calculated by comparing the measured value to the expected amount of Keratin 6A (KRT6A) in samples.
Matrix Recovery range (%) Average(%)
serum(n=5)95-10498
EDTA plasma(n=5)79-8884
heparin plasma(n=5)98-105101
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 6A (KRT6A) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 6A (KRT6A) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Keratin 6A (KRT6A) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
serum(n=5)97-105%86-101%91-103%92-101%
EDTA plasma(n=5)92-102%96-104%84-104%81-98%
heparin plasma(n=5)93-101%82-102%90-104%88-102%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 6A (KRT6A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 6A (KRT6A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 6A (KRT6A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 6A (KRT6A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Package and Components
Assay procedure summary
1. Prepare all reagents, samples and
    standards;
2. Add 100µL standard or sample to each
    well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared
    Detection Reagent A. Incubate 1 hour at
    37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent
    B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15
    -25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm
    immediately.
Reagent Preparation
Results demonstration
Typical Standard Curve
Download
Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAD234Hu21Monoclonal Antibody to Keratin 6A (KRT6A)WB, ICC, IHC-P, IHC-F, ELISA
PAD234Hu01Polyclonal Antibody to Keratin 6A (KRT6A)WB, ICC, IHC-P, IHC-F, ELISA
PAD234Hu71Biotin-Linked Antibody to Keratin 6A (KRT6A)IHC;WB;ELISA
PAD234Hu81FITC-Linked Antibody to Keratin 6A (KRT6A)IHC;ICC;IF;WB
RPD234Hu01Recombinant Keratin 6A (KRT6A)SDS-PAGE; WB; ELISA; IP.
SED234HuELISA Kit for Keratin 6A (KRT6A)Enzyme-linked immunosorbent assay