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ELISA Kit for Retinol Binding Protein 4, Plasma (RBP4) Mus musculus (Mouse) Sandwich ELISA

PRBP; RBP; Plasma retinol-binding protein

  • Product No.SEA929Mu
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range0.312-20ng/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 0.142ng/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Retinol Binding Protein 4, Plasma (RBP4).No significant cross-reactivity or interference between Retinol Binding Protein 4, Plasma (RBP4) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
  • DownloadInstruction Manual
  • UOM 48T96T 96T*5 96T*10 96T*100 In stock
  • FOB US$ 321.00 US$ 458.00 US$ 2,061.00 US$ 3,893.00 US$ 32,060.00
  • Quantity
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  • ELISA Kit for Retinol Binding Protein 4, Plasma (RBP4) Packages (Simulation)
  • ELISA Kit for Retinol Binding Protein 4, Plasma (RBP4) Packages (Simulation)
  • ELISA Kit for Retinol Binding Protein 4, Plasma (RBP4) Results demonstration
  • SEA929Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinol Binding Protein 4, Plasma (RBP4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinol Binding Protein 4, Plasma (RBP4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Retinol Binding Protein 4, Plasma (RBP4)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinol Binding Protein 4, Plasma (RBP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinol Binding Protein 4, Plasma (RBP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinol Binding Protein 4, Plasma (RBP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinol Binding Protein 4, Plasma (RBP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Diabetes Technology & TherapeuticsProteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 Liebert: 20100078
  • Journal of Pharmaceutical and Biomedical AnalysisEffect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2PubMed: 21130593
  • Evid Based Complement Alternat MedQuercetin Protects against Cadmium-Induced Renal Uric Acid Transport System Alteration and Lipid Metabolism Disorder in RatsPubMed: PMC3368504
  • Journal of EndocrinologyCombined galanin with insulin improves insulin sensitivity of diabetic rat musclesPubmed:24501381
  • J EndocrinolCentral alarin ameliorated insulin resistance of adipocytes in type 2 diabetic ratsPubmed:25240061
  • PeptidesEffect of endogenous galanin on glucose transporter 4 expression in cardiac muscle of type 2 diabetic ratsPubmed:25445608
  • Obesity SurgeryLong-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty RatsPubmed:25697126
  • Clin Exp NephrolDifferentially expressed urinary biomarkers in children with idiopathic nephrotic syndromePubMed: 26351173
  • Experimental and Therapeutic MedicineExpression of retinol binding protein 4 and nuclear factor-ÎşB in diabetic rats with atherosclerosis and the intervention effect of pioglitazonePubmed:27446311
  • Experimental and Therapeutic MedicineExpression of retinol binding protein 4 and nuclear factor-κB in diabetic rats with atherosclerosisand the intervention effect of pioglitazone.pubmed:27446311

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