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ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA) Mus musculus (Mouse) Sandwich ELISA

VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor

  • Product No.SEA143Mu
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range15.6-1000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 5.7pg/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor A (VEGFA).No significant cross-reactivity or interference between Vascular Endothelial Growth Factor A (VEGFA) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
  • DownloadInstruction Manual
  • UOM 48T96T 96T*5 96T*10 96T*100 In stock
  • FOB US$ 325.00 US$ 464.00 US$ 2,088.00 US$ 3,944.00 US$ 32,480.00
  • Quantity
  • Add to Cart Distributors
  • ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA) Packages (Simulation)
  • ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA) Packages (Simulation)
  • ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA) Results demonstration
  • SEA143Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Vascular Endothelial Growth Factor A (VEGFA) and the recovery rates were calculated by comparing the measured value to the expected amount of Vascular Endothelial Growth Factor A (VEGFA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-105 102
EDTA plasma(n=5) 86-101 93
heparin plasma(n=5) 96-103 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vascular Endothelial Growth Factor A (VEGFA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-98% 89-96% 79-91% 95-104%
EDTA plasma(n=5) 90-104% 80-99% 96-103% 85-99%
heparin plasma(n=5) 86-98% 86-101% 85-101% 80-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor A (VEGFA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor A (VEGFA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Endothelial Growth Factor A (VEGFA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vascular Endothelial Growth Factor A (VEGFA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • The Journal of Biological ChemistryIntertissue flow of GSH as a tumor growth promoting mechanism: interleukin 6 induces GSH release from hepatocytes in metastatic B16 melanoma-bearing miceJcb: 196261
  • Turkish Journal of Veterinary and Animal SciencesCan the vascular endothelial growth factor (VEGF) and total estradiol 17β be used as a marker of equine oocyte maturation?Tubitak: source
  • Regulatory PeptidesThe correlation between resting serum leptin and serum angiogenic indices at rest and after submaximal exerciseScienceDirect: S016701151100156X
  • Acta BiomaterialiaMultiple release of polyplexes of plasmids VEGF and bFGF from electrospun fibrous scaffolds towards regeneration of mature blood vesselsPubMed: 22484697
  • OncogeneElevated PLGF contributes to small-cell lung cancer brain metastasisPubMed: 22797069
  • International Journal of Nephrology Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with PolyangiitisPubMed: 22792461
  • Zahedan Journal of Research in Medical SciencesThe Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary MenZjrms: Source
  • Nanoscale Research LettersSilver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart.PubMed: 22827927
  • BMC cell biologyBMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopausePubMed: PMC3640998
  • PLoS One.Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome SequencingPubMed: PMC3378547
  • Oncology Reports A bone-seeking clone exhibits different biological properties from the ACHN parental human renal cell carcinoma in vivo and in vitroPubMed: PMC3583590
  • The Journal of Biological ChemistryIntertissue Flow of Glutathione (GSH) as a Tumor Growth-promoting Mechanism: INTERLEUKIN 6 INDUCES GSH RELEASE FROM HEPATOCYTES IN METASTATIC B16 MELANOMA-BEARING MICEPubMed: PMC3091180
  • International Journal of  Molecular SciencesNano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral MusclesNCBI:PMC3856104
  • Archives of Animal NutritionNano-nutrition of chicken embryos. The effect of silver nanoparticles and ATP on expression of chosen genes involved in myogenesisPubmed: 23952606
  • AngiogenesisProdrug of green tea epigallocatechin-3-gallate (Pro-EGCG) as a potent anti-angiogenesis agent for endometriosis in micePubmed: 22948799
  • Experimental Eye ResearchHyperglycemia promotes vasculogenesis in choroidal neovascularization in diabetic mice by stimulating VEGF and SDF-1 expression in retinal pigment epithelial cellsPubmed: 24780853
  • Tumor BiologyBiglycan up-regulated vascular endothelial growth factor (VEGF) expression and promoted angiogenesis in colon cancerPubmed:25371074
  • Archives of Animal NutritionEffect of silver nanoparticles and hydroxyproline, administered in ovo, on the development of blood vessels and cartilage collagen structure in chicken embryosPubmed:25530495
  • Stem Cells InternationalMatrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In VitroPubmed:25767513
  • Physiology and PharmacologyA Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary MenPhypha:Source
  • Int J Clin Exp Med.The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cellPubMed: 26628972
  • Mol Med RepApplication of recombinant peroxisome proliferatorPubMed: 26676891
  • Journal of ImmunotherapyWogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor ImmunityPubMed: 25962106
  • shockAngiogenesis inhibitor endostatin protects mice with sepsis from multiple organ dysfunction syndromePubMed: 26125086
  • Clinical SciencePigment epithelium-derived factor regulates microvascular permeability through adipose triglyceride lipase in sepsisPubMed: 25700221
  • Histol HistopatholEstrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cellsPubMed: 26254457
  • International Journal of OncologySilencing of Livin inhibits tumorigenesis and metastasis via VEGF and MMPs pathway in lung cancerPubMed: 26094984
  • Acta BiomaterAntioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture studyPubMed: 26689470
  • JBiSEHealing Mechanism and Osteogenic Capacity of Bovine Bone Mineral—Human Amniotic Mesenchymal Stem Celland Autogenous Bone Graft in Critical Size Mandibular DefectJournal: 60629
  • BMC ImmunolImmunoexpression of TGF-β/Smad and VEGF-A proteins in serum and BAL fluid of sarcoidosis patientsPubMed: 26445225
  • SCaracterizaTeses: Disponiveis
  • Deletion Polymorphism and Chronic Obstructive Pulmonary DiseaseReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial CellsPubmed:27075970
  • Journal of Controlled ReleasePharmacokinetic and pharmacodynamic study of a phospholipid-based phase separation gel for once a month administration of octreotidePubmed:27040814
  • The Journal of Maternal-Fetal & Neonatal MedicineSerum angiogenic profile in abnormal placentationPubmed:26863111
  • Acta BiomaterialiaPreparation and characterization of pro-angiogenic gel derived from small intestinal submucosaarticle:S1742706115301410
  • Acta BiomaterialiaAntioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture studyPubmed:26689470
  • AlcoholThe effects of ethanol on angiogenesis after myocardial infarction, and preservation of angiogenesis with rosuvastatin after heavy drinkingPubmed:27565753
  • American Journal of Biomedical SciencesInsulin Like Growth Factor -1(IGF-1) Promotes Angiogenesis and Reverses Ischemia Reperfusion Induced Acute Kidney Injury in Rats: Role of VEGF and TGF-β1publication:303524932_Insulin_Like_Growth_Factor_-
  • 临床合理用药 2016 年 8 月第 9 卷第 8A 期袖带法缺血训练对单支冠状动脉慢性完全闭塞病变患者心肌缺血及左心室功能的影响58477c05699e4.pdf
  • The Royal Society of ChemistryEffects of immobilized VEGF on endothelial progenitor cells cultured on silicon substituted and nanocrystalline hydroxyapatitesc6ra19154a
  • J Cell Physiol.An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells.pubmed:28240358

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