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ELISA Kit for Immunoglobulin G (IgG) Rattus norvegicus (Rat) Competition ELISA

  • Product No.CEA544Ra
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Test MethodCompetitive Inhibition
  • Assay Length2h
  • Detection Range1.23-100ug/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 0.49ug/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G (IgG).No significant cross-reactivity or interference between Immunoglobulin G (IgG) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
  • DownloadInstruction Manual
  • UOM 48T96T 96T*5 96T*10 96T*100 In stock
  • FOB US$ 315.00 US$ 450.00 US$ 2,025.00 US$ 3,825.00 US$ 31,500.00
  • Quantity
  • Add to Cart Distributors
  • ELISA Kit for Immunoglobulin G (IgG) Packages (Simulation)
  • ELISA Kit for Immunoglobulin G (IgG) Packages (Simulation)
  • ELISA Kit for Immunoglobulin G (IgG) Results demonstration
  • CEA544Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Immunoglobulin G (IgG) and the recovery rates were calculated by comparing the measured value to the expected amount of Immunoglobulin G (IgG) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-104 101
EDTA plasma(n=5) 87-98 94
heparin plasma(n=5) 80-93 81

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin G (IgG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-92% 96-105% 95-105% 79-90%
EDTA plasma(n=5) 94-105% 94-101% 89-97% 97-105%
heparin plasma(n=5) 83-95% 87-103% 93-102% 80-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Immunoglobulin G (IgG)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Immunoglobulin G (IgG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Immunoglobulin G (IgG) and unlabeled Immunoglobulin G (IgG) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin G (IgG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin G (IgG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Immunoglobulin G (IgG) in the sample.

Citations

  • Journal of Animal and Veterinary AdvancesTryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease VaccineMedwell: 2010610616
  • Journal of Animal and Veterinary AdvancesImmunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease VirusMedwell: 2010594600
  • international Journal of food and nutritional sciencesEVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHIIjfans: Source
  • Poultry ScienceEffects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88Pubmed: 24570422
  • Pharmaceutical Development and TechnologyFoot and Mouth Disease virus-loaded fungal chitosan nanoparticles for intranasal administration: impact of formulation on physicochemical and immunological characteristicsPubmed: 23590209
  • BioMed Research InternationalThe Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt SignalingHindawi:Source
  • Journal of ProteomicsProteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infectionPubmed:24576640
  • Int J Occup Environ Health.Liver functions in silica-exposed workers in Egypt: possible role of matrix remodeling and immunological factorsPubmed:24999850
  • Food &amp; FunctionDietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic micePubmed:25308407
  • Oncology 
ReportsBeneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancerPubmed:25529861
  • Biomed Res IntThe Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt SignalingPubMed: 26161394
  • Protein Pept LettStabilization of Human Immunoglobulin G Encapsulated within Biodegradable Poly (Cyclohexane-1, 4-diyl Acetone Dimethylene Ketal) (PCADK)/ Poly (Lactic-co-Glycolic Acid) (PLGA) Blend MicrospheresPubMed: 26268563
  • ElectrophoresisGrowth promotion in pigs by oxytetracycline coincides with down reCavia (Guinea pig )lation of serum inflammatory parameters and of hibernation‐associated protein HP‐27Pubmed:26914286
  • NeuropeptidesNeuropeptide Y stimulates osteoblastic differentiation and VEGF expression of bone marrow mesenchymal stem cells related to canonical Wnt signaling activating in vitro.Pubmed:26707636
  • Archives of Biological SciencesEffect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestinepid:46641600021Z
  • Asian J Pharm Clin ResINFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATSjournals:3790
  • journal of biotechnologyExpression of α-1,6-fucosyltransferase (FUT8) in rice grain and immunogenicity evaluation of plant-specific glycans.pubmed:28013072
  • Sci Rep. Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system.pubmed:28220870
  • Toxicology ResearchEffect of dietary protein or calcium supplement on the expression of collagen I and dentine phosphoprotein of rats with dental fluorosisC6TX00288A
  • ntervirologyExposure of Piglets to Enteroviruses Investigated by an Immunoassay Based on the EV-G1 VP4 Peptidepubmed:27694750
  • Biomedicine & PharmacotherapyIcariin promotes osteogenic differentiation of rat bone marrow stromal cells by activating the ERα-Wnt/β-catenin signaling pathway.pubmed:27764755

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