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ELISA Kit for Insulin Like Growth Factor 1 (IGF1) Rattus norvegicus (Rat) Sandwich ELISA

IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor

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  • ELISA Kit for Insulin Like Growth Factor 1 (IGF1) Packages (Simulation)
  • ELISA Kit for Insulin Like Growth Factor 1 (IGF1) Packages (Simulation)
  • ELISA Kit for Insulin Like Growth Factor 1 (IGF1) Results demonstration
  • SEA050Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Insulin Like Growth Factor 1 (IGF1) and the recovery rates were calculated by comparing the measured value to the expected amount of Insulin Like Growth Factor 1 (IGF1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-101 97
EDTA plasma(n=5) 93-101 97
heparin plasma(n=5) 78-99 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Insulin Like Growth Factor 1 (IGF1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-102% 93-105% 79-90% 78-101%
EDTA plasma(n=5) 79-101% 92-101% 84-95% 89-98%
heparin plasma(n=5) 78-101% 95-103% 82-101% 81-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Insulin Like Growth Factor 1 (IGF1)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 1 (IGF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 1 (IGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 1 (IGF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 1 (IGF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapyPubMed: 20119855
  • Characterization of the specific and sustained GH1 expression induced by rAAV2/1 in normal adult male ratsPubMed: 20204528
  • The vagus nerve as a regulator of growth hormone secretionPubMed: 21056594
  • Insulin-Like Growth Factor-1 (IGF-1) Reduces Ischemic Changes and Increases Circulating Angiogenic Factors in Experimentally - Induced Myocardial Infarction in RatsPubMed: 21651821
  • Physical activity attenuates intermittent hypoxia-induced spatial learning deficits and oxidative stress.PubMed: 20224062
  • The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass modelPubMed: 20438940
  • Serum insulin-like growth factor type 1 concentrations in healthy dogs and dogs with spontaneous primary hypothyroidismPubMed: 21546289
  • Effects of monochromatic light on developmental changes in satellite cell population of pectoral muscle in broilers during early posthatch periodWiley: source
  • Interleukin-6 Regulates the Expression of Hypothalamic Neuropeptides Involved in Body Weight in a Gender-Dependent WayWiley: source
  • Insulin Like Growth Factor-1 (IGF-1) Causes Overproduction of IL-8, an Angiogenic Cytokine and Stimulates Neovascularization in Isoproterenol-Induced Myocardial Infarction in RatsMdpi: 8562
  • Protective Effect of Amlodipine on Rat Bone Tissue after OrchidectomyKarger: 335491
  • Maternal Glucocorticoid Treatment as a Model for Examining Foetal Gender-Specific Effects on the Bone Metabolism of Neonatal PigletsDegruyter: Source
  • BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopausePubMed: PMC3640998
  • Evaluation of IGF-I levels and serum protein profiles of diabetic cats and dogsPubMed: PMC3232391
  • A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
  • Porcine follicular fluid concentration of free insulin-like growth factor-I collected from different diameter ovarian folliclesScielo: Source
  • EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHIIjfans: Source
  • Role of insulin-like growth factor-I and follicular fluid from ovarian follicles with different diameters on porcine oocyte maturation and fertilization in vitroScienceDirect: S0093691X13001659
  • Electrochemical immunosensor for the determination of insulin-like growth factor-1 using electrodes modified with carbon nanotubes–poly(pyrrole propionic acid) hybridsScienceDirect: S0956566313005629
  • The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar ratsInformahealthcare: Source
  • A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor BiomarkersTandfonline: Source
  • CONCENTRACOES SéRICAS DE INSULINA NO PóS-PARTO RECENTE DE VACAS DE CORTE DE DIFERENTES GENóTIPOS CRIADAS NO SUL DO BRASILEmbrapa: Source
  • Negative Energy Balance Induced by Paradoxical Sleep Deprivation Causes Multicompartmental Changes in Adipose Tissue and Skeletal MuscleCepebr:Source
  • The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism.Pubmed:24629477
  • Effects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary SyndromePubmed:25667201
  • Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetesPubMed: 26379831
  • The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cellPubMed: 26628972
  • The use of herb mix and lucerne to increase growth rates of dairy heifersSystem: Files
  • Maternal Nutrition with Β-Hydroxy-Β-Methylbutyrate as Strong Determinants of the Development of Newborn Offspring in PigsJournals: 1000183
  • The Protective Effects of Exclusive Enteral Nutrition Formulas on Growth Factor Expression and the Proximal Tibial Epiphyseal Growth Plate in a TNBS-Induced IBD Rat ModelPubMed: 25708898
  • Hypoxia-induced secretion of IL-12 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphomaPubMed: 26695151
  • RELATIONSHIP OF RESIDUAL FEED INTAKE WITH BLOOD METABOLITES AND HORMONES IN SAHIWAL FEMALE CALVESWp-Content: Uploads
  • CaracterizaTeses: Disponiveis
  • Melatonin Mediates Monochromatic Light‐induced Insulin‐like Growth Factor 1 Secretion of Chick Liver: Involvement of Membrane ReceptorsPubmed:27128575
  • Protective effect of vitamin D against hyperoxia‐induced lung injury in newborn ratsPubmed:27291304
  • A single bout of resistance exercise improves memory consolidation and increases the expression of synaptic proteins in the hippocampusPubmed:27008926
  • Cigarette Smoke Delays Regeneration of the Olfactory Epithelium in MicePubmed:27003941
  • Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87
  • Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickensview:87
  • Effects of acute exercise on salivary free insulin-like growth factor 1 and interleukin 10 in sportsmen.pubmed:27605972
  • Paradoxical Sleep Deprivation Causes Cardiac Dysfunction and the Impairment Is Attenuated by Resistance Training.pubmed:27880816
  • Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors.pubmed:28216608
  • Chronic intermittent hypoxia disturbs insulin secretion and causes pancreatic injury via theMAPK signaling pathway.pubmed:28177762
  • Resistance Exercise Reduces Seizure Occurrence, Attenuates Memory Deficits and Restores BDNF Signaling in Rats with Chronic Epilepsyarticle:10.1007
  • GH蛋白诱导鸡原代成肌细胞表达IGF1条件优化10697
  • Assessment of feeding varying levels of Metabolizable energy and protein on performance of transition Murrah buffaloespubmed:28785907
  • Mesenchymal stem cells promote endothelial progenitor cell proliferation by secreting insulin‑like growth factor‑1pubmed:28627605
  • Resistance Exercise Reduces Seizure Occurrence, Attenuates Memory Deficits and Restores BDNF Signaling in Rats with Chronic Epilepsy pubmed:28078614
  • Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Dietarticle:10.1007
  • Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken10.159:1806-9061-2016-0285
  • Studies on quality attributes of skimmed colostrum powderP-ISSN: 2349–8528
  • Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats10.1186/s13287-017-0739-3
  • A metabolite of leucine (β-hydroxy-β-methylbutyrate) given to sows during pregnancy alters bone development of their newborn offspring by hormonal modulation10.1371/journal.pone.0179693
  • A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injurypubmed:28522195
  • Effects of Bariatric Surgery on Change of Brown Adipocyte Tissue and Energy Metabolism in Obese Micepubmed:28853013
  • Effect of dietary protein intake on the body composition and metabolic parameters of neutered dogspubmed:29152244
  • Involvements of γδT Lymphocytes in Acute and Chronic Skin Wound Repair.pubmed:28540539
  • Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy and Inhibiting Excessive Astrocytic Apoptosis.pubmed:28959186
  • Various LED Wavelengths Affected Myofiber Development and Satellite Cell Proliferation of Chick Embryos via the IGF-1 Signaling Pathway.pubmed:28708285
  • An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signalingPubmed:29627530
  • Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transferPubmed:29482569
  • Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickensPubmed:29373588
  • Evaluation of serum insulin-like growth factor-1 and 26S proteasome concentrations in healthy dogs and dogs with chronic diseases depending on body condition …10.1016:j.rvsc.2018.04.009
  • Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive RatsPubmed:29898457
  • Dietary supplementation for Santa Inês hair ewes on pasture at pre-and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolismPubmed:29931604
  • 26S proteasome and insulin-like growth factor-1 in serum of dogs suffering from malignant tumorsPubmed:29755191
  • Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs.:
  • Circulating concentrations of insulin like growth factor-1 in female dogs with spontaneous mammary tumours10.21521:mw.6054
  • Nucleoside reverse transcriptase inhibitor-induced rat oocyte dysfunction and low fertility mediated by autophagyPubmed:29423092
  • Effect of porcine somatotropin on metabolism, testicular size and sperm characteristics in young boars10.1590:1678-4162-9481
  • Fermented corn-soybean meal elevated IGF1 levels in grower-finisher pigsPubmed: 30203098
  • Maternal HMB treatment affects bone and hyaline cartilage development in their weaned piglets via the leptin/osteoprotegerin systemPubmed: 30659706
  • The therapeutic effects of nicotinamide in hepatocellular carcinoma through blocking IGF-1 and effecting the balance between Nrf2 and PKBPubmed: 30784932
  • Association between Insulin-Like Growth Factor-I Levels and the Disease Progression and Anemia in Visceral LeishmaniasisPubmed: 30761980
  • Light-dark rhythms during incubation of broiler chicken embryos and their effects on embryonic and post hatch leg bone developmentPubmed: 30682088
  • Mel1c Mediated Monochromatic Light-Stimulated IGF-I Synthesis through the Intracellular Gαq/PKC/ERK Signaling PathwayPubmed: 30987295
  • Serum and seminal plasma IGF-1 associations with semen variables and effect of IGF-1 supplementation on semen freezing capacity in buffalo bullsPubmed: 30914211
  • Melatonin mediates monochromatic green light-induced satellite cell proliferation and muscle growth in chick embryoPubmed: 31059537
  • Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and …
  • Alterations in haemato-biochemical profile following by-pass nutrients supplementation in early lactating Murrah buffaloes
  • Effect of betaine supplementation on growth performance, nutrient intake and expression of IGF-1 in Karan Fries heifers during thermal stressPubmed: 31711708
  • Effects of a diet enriched with eicosapentaenoic, docosahexaenoic and glutamine on cytokines as immunological markers for systemic inflammation in bitches before …Pubmed: 31637790
  • The association between serum insulin-like growth factor 1 and cognitive impairments in patients with schizophreniaPubmed: 31839419
  • BST Stimulation Induces Atrophy and Changes in Aerobic Energy Metabolism in Rat Skeletal Muscles—The Biphasic Action of Endogenous GlucocorticoidsPubmed: 32316389
  • Fenugreek Stimulates the Expression of Genes Involved in Milk Synthesis and Milk Flow through Modulation of Insulin/GH/IGF-1 Axis and Oxytocin SecretionPubmed: 33081164
  • Cytokine and Growth Factor Delivery from Implanted Platelet-Rich Fibrin Enhances Rabbit Achilles Tendon HealingPubmed: 32370144
  • Is Dietary 2-Oxoglutaric Acid Effective in Accelerating Bone Growth and Development in Experimentally-Induced Intrauterine Growth Retarded Gilts?Pubmed: 32331362
  • Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy …
  • Changes and Risk Factors of Skeletal Muscle Mass and Strength in Patients with Type 2 Diabetes over 60 Years Old: A Cross-Sectional Study from China
  • Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and
  • INFLUENCE OF OMEGA–3 RICH FATTY ACID ENRICHED FEED ON BIOCHEMICAL MARKERS AND POST PARTUM REPRODUCTIVE PERFORMANCE OF …
  • Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care33469783
  • Adipose-Derived Stem Cells Improve the Aging Skin of Nude Mice by Promoting Angiogenesis and Reducing Local Tissue Water33428732
  • Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves33610512
  • Handling Associated with Drenching Does Not Impact Survival and General Health of Low Birth Weight Piglets. Animals 2021, 11, 40433562568
  • New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density33842739
  • The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin?¡­33381190
  • The Protective Role of Alpha-Ketoglutaric Acid on the Growth and Bone Development of Experimentally Induced Perinatal Growth-Retarded Piglets33435211
  • Comparative study on genital status, energy balance and mineral content of swamp buffalo (Luit) heifers and cows under organized system of rearing in Assam?¡­
  • The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study34348809
  • Effects of Concentrate and Bypass Fat Supplementations on Growth Performance, Blood Profile, and Rearing Cost of Feedlot Buffaloes34359233
  • Association of Salivary IGF and IGF/IGFBP-3 Molar Ratio with Cervical Vertebral Maturation Stages from Pre-Adolescent to Post-Adolescent Transition Period—A …Pubmed:35564566

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