ELISA Kit for Interleukin 17 (IL17) Rattus norvegicus (Rat) Sandwich ELISA

IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8

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  • ELISA Kit for Interleukin 17 (IL17) Packages (Simulation)
  • ELISA Kit for Interleukin 17 (IL17) Packages (Simulation)
  • ELISA Kit for Interleukin 17 (IL17) Results demonstration
  • SEA063Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 17 (IL17) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 17 (IL17) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-93 83
EDTA plasma(n=5) 78-90 85
heparin plasma(n=5) 94-105 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 17 (IL17) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-98% 98-105% 78-99% 92-101%
EDTA plasma(n=5) 78-103% 78-92% 83-99% 98-105%
heparin plasma(n=5) 87-94% 80-97% 94-105% 94-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Interleukin 17 (IL17)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 17 (IL17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 17 (IL17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 17 (IL17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 17 (IL17) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Impaired Autoimmune T Helper 17 Cell Responses Following DNA Vaccination against Rat Experimental Autoimmune EncephalomyelitisPubMed: PMC2577303
  • Atorvastatin ameliorates experimental autoimmune neuritis by decreased Th1/Th17 cytokines and up-regulated T regulatory cellsScienceDirect: S0008874911002164
  • Improvement by Phytotherapeutic Agent of Detrusor Overactivity, Down-Regulation of Pharmacological Receptors and Urinary Cytokines in Rats with Cyclophosphamide Induced CystitisPubMed: S0022534712049087
  • Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhagePubMed: PMC3383056
  • Effects of Saw Palmetto Extract on Urodynamic Parameters, Bladder Muscarinic and Purinergic Receptors and Urinary Cytokines in Rats with Cyclophosphamide-Induced CystitisOnlinelibrary: luts.12015
  • Expression of Hemopexin in Acute Rejection of Rat Liver Allograft Identified by Serum Proteomic Analysis.Lww: Source
  • CD4+ T Cell Cytokine Gene and Protein Expression in Duodenal Mucosa of Dogs with Inflammatory Bowel DiseasePubmed:24270804
  • Increased blood-circulating interferon-γ, interleukin-17, and osteopontin levels in bovine paratuberculosisPubmed:24679935
  • Pemetrexed Ameliorates Experimental Arthritis in RatsPubmed:25129058
  • Lapatinib Ameliorates Experimental Arthritis in RatsPubmed:25283886
  • Beta-arrestin-2 negatively modulates inflammation response in mouse chondrocytes induced by 4-mer hyaluronan oligosaccharidePubmed:25318610
  • Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosisPubmed:25323532
  • Protective effect and mechanism of probucol in the treatment of spinal cord injuryPubMed: 26214485
  • Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cellsArticle_14936_6.Html:
  • Hypoxia-induced secretion of IL-20 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphomaPubMed: 26695151
  • MicroRNA‐155 may be involved in the pathogenesis of atopic dermatitis by modulating the differentiation and function of T helper type 17 (Th18) cellsPubMed: 25761610
  • The immunomodulating effect of seminal plasma on T cellsPubMed: 25799173
  • Interleukin-17 levels correlate with poor prognosis and vascular endothelial growth factor concentration in the serum of patients with non-small cell lung cancerPubMed: 26301881
  • Tanshinone IIA attenuates experimental autoimmune encephalomyelitis in ratsPubmed:27357729
  • Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression ofHMGB1/RAGE/NF-ΚB pathway.Pubmed:27026909
  • Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgeryPubmed:26802602
  • Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells10610
  • Immunological and pathological investigations in equine experimental uveitispubmed:27344152
  • Th17-skewed immune response and cluster of differentiation 40 ligand expression in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.pubmed:28114998
  • Anti-inflammatory effects of Boletus edulis polysaccharide on asthma pathologypubmed:27830033
  • Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-ΚB pathwaypubmed:27026909
  • Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys.pubmed:
  • Relationship between IL-17 serum level and ambulatory blood pressure in women with polycystic ovary syndromepubmed:28042549
  • Relationship between IL-17 and ambulatory blood pressure in polycystic ovary syndrome8b168a09f5832df1b5c2f48631612458b2d8.pdf
  • Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling.pubmed:28032440
  • Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in micepubmed:28250847
  • Procalcitonin und proinflammatorische Zytokine in der bronchoalveolären Lavage beim lungenkranken Pferd unter inhalativer GlukokortikoidtherapieFUDISS_thesis_000000103830
  • Anti-asthmatic efect of pitavastatin through aerosol inhalation is associated with CD4+ CD25+ Foxp3+ T cells in an asthma mouse modelpubmed:28729731
  • Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signallingdoi:10.1111
  • Antiinflammatory and antioxidant effects of gemcitabine in collagen-induced arthritis modelpubmed:28618762
  • Low-density neutrophils in severe fever with thrombocytopenia syndrome (SFTS) display decreased function to phagocytose SFTS virus and enhanced capacity to synthesize cytokinesISSN:1936-2625/IJCEP0045334
  • Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in micepubmed:28122397
  • Increased Levels of IL-17, IL-23, MIP-1α, MCP-1 and Global Leukocytes in Fibromyalgia Patients10.1080/24708593.2017.1357664
  • Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia.pubmed:29257310
  • Interleukin-17 antagonist attenuates lung inflammation through inhibition of the ERK1/2 and NF-κB pathway in LPS-induced acute lung injury10.3892/mmr.2017.6837
  • Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosispubmed:28608724
  • Therapeutic effect of bone marrow mesenchymal stem cells pretreated with acetylsalicylic acid on experimental periodontitis in ratspubmed:29195233
  • Associations among interleukin-17, interferon-gamma, and acute coronary syndromeISSN 0970-938X
  • Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory …Pubmed:29672286
  • Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal …Pubmed:29375374
  • Decreased miR-128 expression alleviated the progression of Rheumatoid arthritis by up-regulating the expression of TNFAIP3Pubmed:29853534
  • Involvement of T-Helper 9 Activation in a Mouse Model of Allergic RhinitisPubmed:29982264
  • Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary diseasePubmed:29950127
  • Inhibition of microRNA-155 attenuates concanavalin A-induced autoimmune hepatitis by regulating Treg/Th17 cell differentiationPubmed: 30290123
  • Nystatin enhances the immune response against Candida albicans and protects the ultrastructure of the vaginal epithelium in a rat model of vulvovaginal …Pubmed: 30359236
  • Recombinant adiponectin alleviates abortion in mice by regulating Th17/Treg imbalance via p38MAPK-STAT5 pathwayPubmed: 30496353
  • Enhanced antifungal activity of bovine lactoferrin-producing probiotic Lactobacillus casei in the murine model of vulvovaginal candidiasisPubmed: 30621597
  • Negative regulation of glial Tim‐3 inhibits the secretion of inflammatory factors and modulates microglia to antiinflammatory phenotype after experimental intracerebral?…Pubmed: 30677253
  • Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus
  • Local probiotic Lactobacillus crispatus and Lactobacillus delbrueckii exhibit strong antifungal effects against vulvovaginal candidiasis in a rat model
  • TRAF-6 调控TGF-β1-Smad2/Smad3 信号通路在Graves 病免疫发病机制中的作用
  • Therapeutic effectiveness of type I interferon in vulvovaginal candidiasisPubmed: 31158491
  • Protein Kinase C Theta Inhibition Attenuates Lipopolysaccharide-Induced Acute Lung Injury through Notch Signaling Pathway via Suppressing Th17 Cell Response …Pubmed: 31297750
  • Adenovirus-mediated down-regulation of miR-21-5p alleviates experimental autoimmune uveoretinitis in micePubmed: 31228813
  • Untersuchung einer Th17-vermittelten Immunantwort bei der kaninen idiopathischen Epilepsie
  • Vitamin D protects against oxidative stress, inflammation and hepatorenal damage induced by acute paracetamol toxicity in ratPubmed: 31255668
  • Indoleamine 2, 3-Dioxygenase (IDO) Regulates Th17/Treg Immunity in Experimental IgA Nephropathy
  • Anti-Inflammatory State in Arabian Horses Introduced to the Endurance TrainingPubmed: 31462005
  • Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritisPubmed: 31641939
  • Epigallocatechin 3‐gallate attenuates arthritis by regulating Nrf‐2, HO‐1 and cytokine levels in an experimental arthritis modelPubmed: 31746064
  • Tangeretin attenuates lipopolysaccharide-induced acute lung injury through Notch signaling pathway via suppressing Th17 cell response in micePubmed: 31676364
  • Effects of thalidomide on Th17, Treg cells and TGF‐β1/Smad3 pathway in a mouse model of systemic sclerosisPubmed: 31840939
  • Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitisPubmed: 31927460
  • The therapeutic effect of dendritic cells expressing indoleamine 2, 3-dioxygenase (IDO) on an IgA nephropathy mouse modelPubmed: 31894556
  • Salivary Del‐1, IL‐17, and LFA‐1 levels in periodontal health and diseasePubmed: 32153040
  • LncRNA Profile in Hashimoto's Thyroiditis and Potential Function of NONHSAT079547. 2Pubmed: 32229697
  • Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt/GSK-3β/β-catenin signalingPubmed: 33171191
  • Rhodiola crenulata reduces ventricular arrhythmia through mitigating the activation of IL-17 and inhibiting the MAPK signaling pathwayPubmed: 32946036
  • Effects of 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary CholangitisPubmed: 32908870
  • Covering the proximal nerve stump with chondroitin sulfate proteoglycans prevents traumatic painful neuroma formation by blocking axon regeneration after …Pubmed: 32470939
  • Melatonin attenuates microbiota dysbiosis of jejunum in short-term sleep deprived micePubmed: 32424577
  • Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in ratPubmed: 32395767
  • Vitamin D3 and calcium cosupplementation alleviates cadmium hepatotoxicity in the rat: Enhanced antioxidative and anti‐inflammatory actions by remodeling cellular …
  • A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic …
  • Dietary Malted Barley Grain Improved Structure and Function of Spinal Cord of Mother Rats fed on a high cholesterol diet.
  • Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction
  • Effects of minocycline on neuroinflammation, gut microbiome and hippocampal neurogenesis in rats with Gulf War Illness
  • Minocycline Alleviate A Rat Model Of Gulf War Illness Via Altering Gut Microbiome, Attenuating Neuroinflammation And Enhancing Hippocampal Neurogenesis
  • 丹参对非酒精性脂肪肝Th17细胞及相关细胞因子的影响*
  • A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic?¡­33295414
  • Minocycline alleviates Gulf War Illness rats via altering gut microbiome, attenuating neuroinflammation and enhancing hippocampal neurogenesis34000339
  • Serum Galectin©\3 Levels in Patients with Psoriasis34137138
  • The Effect of the Clenbuterol¡ª¦Â2-Adrenergic Receptor Agonist on the Peripheral Blood Mononuclear Cells Proliferation, Phenotype, Functions, and Reactive Oxygen?¡­33920705
  • Sparstolonin B Exerts Therapeutic Effects on Collagen-Induced Arthritis by Inhibiting the NLRP3 Inflammasome and Reducing the Activity of ¦Á1, 3 ¡­
  • Alterations in the gut microbiome with hemorrhagic transformation in experimental stroke34591349
  • Evaluation of the involvement of Th17-cells in the pathogenesis of canine spinal cord injury34591917
  • Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study34730850
  • Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive …34802039
  • Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice34960627
  • Th17 cell-mediated immune response in a subpopulation of dogs with idiopathic epilepsyPubmed:35025939
  • Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus PatientsPubmed:35056005
  • Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axisPubmed:35453007
  • The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammalPubmed:35379849
  • Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthmaPubmed:35549595

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