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ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1) Rattus norvegicus (Rat) Sandwich ELISA

TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide

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  • ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1) Packages (Simulation)
  • ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1) Packages (Simulation)
  • ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1) Results demonstration
  • SEA124Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Transforming Growth Factor Beta 1 (TGFb1) and the recovery rates were calculated by comparing the measured value to the expected amount of Transforming Growth Factor Beta 1 (TGFb1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-103 101
EDTA plasma(n=5) 83-93 89
heparin plasma(n=5) 83-104 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transforming Growth Factor Beta 1 (TGFb1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-97% 97-104% 95-105% 98-105%
EDTA plasma(n=5) 96-103% 83-96% 78-93% 79-101%
heparin plasma(n=5) 92-101% 78-101% 88-103% 86-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta 1 (TGFb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta 1 (TGFb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Metformin attenuates cardiac fibrosis by inhibiting the TGFβ1–Smad3 signalling pathwayOxfordJournals: source
  • Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by trinitrobenzene-sulfonic acidScienceDirect: S0188440910001049
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  • Incorporating pTGF-β1/calcium phosphate nanoparticles with fibronectin into 3-dimensional collagen/chitosan scaffolds: efficient, sustained gene delivery to stem cells for chondrogenic differentiation.Pubmed: 22314694
  • A Soluble Antigen-Specific Factor from CD4+CD25+ T Cells of OVA Tolerant Mice Inducing OVA-Specific Peripheral Tolerance Independently in vivoMedwell: 2011642650
  • Increased circulating concentrations of PDGF-BB and TGF-β1 in canine generalised demodicosisRevmedvet: Source
  • The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS RatsBesjournal: Source
  • Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial PneumoniasPubMed: PMC3551240
  • A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
  • Fibrogenic response of hepatic stellate cells in ovarectomised rats exposed to ketogenic dietPubmed: 23568967
  • EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHIIjfans: Source
  • Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats.Pubmed: 24211397
  • Development of a KLD-12 polypeptide/TGF-β1-tissue scaffold promoting the differentiation of esenchymal stem cell into nucleus pulposus-like cells for treatment of intervertebral disc degenerationIjcep:Source
  • Prolactin anterior pituitary expression and circulating levels are reduced in obese and diabetic rats: Role of TGFβ and TNFαPubmed:25715833
  • PAR2, IL4R, TGFβ and TNFα in bronchoalveolar lavage distinguishes extrinsic allergic alveolitis from sarcoidosisPubmed:Pmc4079423
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  • A novel association between TGFb1 and ADAMTS4 in coronary artery disease: A new potential mechanism in the progression of atherosclerosis and diabetes.Pubmed:25592103
  • Targeting Apoptosis, Advanced Glycation End Products and Transforming Growth Factor-β1 by Biguanides and Resveratrol to Ameliorate Experimentally-induced Diabetic NephropathyPdf: Drugs
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  • Development of a KLD-12 polypeptide/TGF-β1-tissue scaffold promoting the differentiation of mesenchymal stem cell into nucleus pulposus-like cells for treatment of intervertebral disc degeneration.PubMed: 25972996
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  • Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure pubmed:29121396
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  • Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescriptionpubmed:28672924
  • Tumor-associated macrophages promote tumor metastasis via the TGF-β/SOX9 axis in non-small cell lung cancerpubmed:29245941
  • Studies on quality attributes of skimmed colostrum powderP-ISSN: 2349–8528
  • Follistatin like-1 aggravates silicainduced mouse lung injurypubmed:28341862
  • Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathwaypubmed:28698735
  • Pigment epithelium‑derived factor protects human glomerular mesangial cells from diabetes via NOXO1‑iNOS suppression10.3892/mmr.2017.7563
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  • Exendin-4 Induces Bone Marrow Stromal Cells Migration Through Bone Marrow-Derived Macrophages Polarization via PKA-STAT3 Signaling Pathwaypubmed:29216639
  • The promising effect of linagliptin and/or indole-3-carbinol on experimentally-induced polycystic ovarian syndrome.pubmed:28619389
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  • TGF-β1 enhances phagocytic removal of neuron debris and neuronal survival by olfactory ensheathing cells via integrin/MFG-E8 signaling pathway10.1016/j.mcn.2017.08.006
  • Systemic and Tumor Th1 and Th2 Inflammatory Profile and Macrophages in Lung Cancer: Influence of Underlying Chronic Respiratory Disease.pubmed:27793775
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  • Lipopolysaccharide enhances TGF‐β1 signalling pathway and rat pancreatic fibrosisPubmed:29424488
  • Quercetin ameliorates pulmonary fibrosis by inhibiting SphK1/S1P signalingPubmed:29940125
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  • Hypoxia-inducible factor-1α activates transforming growth factor-β1/Smad signaling and increases collagen deposition in dermal fibroblastsPubmed:29423039
  • Activated hepatic stellate cells promote epithelial-to-mesenchymal transition in hepatocellular carcinoma through transglutaminase 2-induced pseudohypoxia
  • Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in …Pubmed: 30353332
  • Mechanistic insights to the cardioprotective effect of Blueberry Nutraceutical Extract in Isoprenaline-Induced Cardiac Hypertrophy
  • Evaluation of T regulatory lymphocytes and serum concentration of selected cytokines in dogs with perianal tumors
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  • Anti-inflammatory and cognitive effects of interferon-β1a (IFNβ1a) in a rat model of Alzheimer's diseasePubmed: 30777084
  • Periostin contributes to renal and cardiac dysfunction in rats with chronic kidney disease: reduction of PPARαPubmed: 30890453
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  • Network pharmacology oriented study reveals inflammatory state-dependent dietary supplement hepatotoxicity responses in normal and diseased ratsPubmed: 31140472
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  • Hyperoside protects against heart failure-induced liver fibrosis in ratsPubmed: 31353051
  • Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart FailurePubmed: 31466059
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  • miR-181b-5p inhibits endothelial-mesenchymal transition in monocrotaline-induced pulmonary arterial hypertension by targeting endocan and TGFBR1Pubmed: 31734320
  • CD4+ CD25+ Tregs as dependent factor in the course of bleomycin-induced pulmonary fibrosis in micePubmed: 31678213
  • Lipoxin A4 inhibited the activation of Hepatic Stellate Cells-T6 cells by modulating profibrotic cytokines and NF-κB signaling pathwayPubmed: 31698141
  • Effects of thalidomide on Th17, Treg cells and TGF‐β1/Smad3 pathway in a mouse model of systemic sclerosisPubmed: 31840939
  • Altered expression of nuclear factor of activated T cells, Forkhead Box P3 and immune suppressive genes in regulatory T cells of generalized vitiligo patientsPubmed: 31917889
  • Rapamycin promotes endothelial–mesenchymal transition during stress-induced premature senescence through the activation of autophagyPubmed: 32164764
  • Soufeng Yuchuan decoction mitigates the ovalbumin-induced lung damage in a rat model of asthmaPubmed: 32036215
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