ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA) Rhesus monkey (Simian) Sandwich ELISA

IL1RN; IL1-RA; ICIL1RA; IL1ra3; IL1F3; IRAP;IL-1RA; Interleukin-1 Family Member 3; IL1 inhibitor; Anakinra

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  • ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA) Packages (Simulation)
  • ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA) Packages (Simulation)
  • ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA) Results demonstration
  • SEA223Si.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 1 Receptor Antagonist (IL1RA) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 1 Receptor Antagonist (IL1RA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-97 91
EDTA plasma(n=5) 87-96 91
heparin plasma(n=5) 91-102 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Receptor Antagonist (IL1RA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Receptor Antagonist (IL1RA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 1 Receptor Antagonist (IL1RA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-101% 97-104% 86-96% 83-104%
EDTA plasma(n=5) 94-102% 99-105% 78-99% 80-95%
heparin plasma(n=5) 80-104% 87-102% 78-90% 78-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Antagonist (IL1RA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Antagonist (IL1RA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Antagonist (IL1RA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Antagonist (IL1RA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Mucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel diseaseScienceDirect: S1090023312000962
  • Daily quercetin supplementation over a period of two weeks results in a moderate accumulation of total plasma flavonols in horsesResearchgate:Source
  • Synovial Fluid and Serum Concentrations of Interleukin-1 Receptor Antagonist and Interleukin-1Science: Article
  • A Two-Week Quercetin Supplementation in Horses Results in Moderate Accumulation of Plasma Flavonol ConcentrationsScience: Article
  • Evidence-based review of efficacy and adverse effects of joint medication and evaluation of synovial fluid and serum markers for osteoarthritis in the horsereceive:101658
  • Interleukin 1 receptor antagonist and 2′-5′-oligoadenylate synthetase-like molecules as novel biomarkers for multiple sclerosis patients in Bahrainpubmed:29141788
  • A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmaticspubmed:28822267
  • Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young …Pubmed:29379533
  • Effect of interleukin‐1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel diseasePubmed:29572935
  • M10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in MicePubmed: 33041798

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