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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
ELISA Kit for Solute Carrier Family 30, Member 10 (SLC30A10)
ZnT-10; ZRC1; ZNT8; Zinc Transporter 8
Organism species Mus musculus (Mouse)
Product No.SEN188Mu
Sample typeTissue homogenates and other biological fluids.
Format96-well strip plate
Assay length4.5 hours
Detection range0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL
SensitivityThe minimum detectable dose of this kit is typically less than 0.072ng/mL.
Specificity
This assay has high sensitivity and excellent specificity for detection of Solute Carrier Family 30, Member 10 (SLC30A10).
No significant cross-reactivity or interference between Solute Carrier Family 30, Member 10 (SLC30A10) and analogues was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Solute Carrier Family 30, Member 10 (SLC30A10) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Solute Carrier Family 30, Member 10 (SLC30A10) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 30, Member 10 (SLC30A10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Solute Carrier Family 30, Member 10 (SLC30A10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 30, Member 10 (SLC30A10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Solute Carrier Family 30, Member 10 (SLC30A10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Package and Components
Assay procedure summary
1. Prepare all reagents, samples and
    standards;
2. Add 100µL standard or sample to each
    well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared
    Detection Reagent A. Incubate 1 hour at
    37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent
    B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15
    -25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm
    immediately.
Reagent Preparation
Results demonstration
Typical Standard Curve
Download
Benchmark price
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications
PAN188Mu01Polyclonal Antibody to Solute Carrier Family 30, Member 10 (SLC30A10)IWB, ICC, IHC-P, IHC-F, ELISA
PAN188Mu02Polyclonal Antibody to Solute Carrier Family 30, Member 10 (SLC30A10)IWB, ICC, IHC-P, IHC-F, ELISA
RPN188Mu01Recombinant Solute Carrier Family 30, Member 10 (SLC30A10)SDS-PAGE; WB; ELISA; IP.
RPN188Mu02Recombinant Solute Carrier Family 30, Member 10 (SLC30A10)SDS-PAGE; WB; ELISA; IP.
SEN188MuELISA Kit for Solute Carrier Family 30, Member 10 (SLC30A10)Enzyme-linked immunosorbent assay