ELISA Kit for Testosterone (Testo) Pan-species (General) Competition ELISA

T; Testesteron; Testeronun

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  • ELISA Kit for Testosterone (Testo) Packages (Simulation)
  • ELISA Kit for Testosterone (Testo) Packages (Simulation)
  • ELISA Kit for Testosterone (Testo) Results demonstration
  • CEA458Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Testosterone (Testo) and the recovery rates were calculated by comparing the measured value to the expected amount of Testosterone (Testo) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 101
EDTA plasma(n=5) 98-105 101
heparin plasma(n=5) 98-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Testosterone (Testo) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Testosterone (Testo) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Testosterone (Testo) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-104% 88-96% 91-99% 80-98%
EDTA plasma(n=5) 87-98% 99-105% 92-101% 90-99%
heparin plasma(n=5) 80-91% 91-99% 81-91% 90-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Testosterone (Testo)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Testosterone (Testo) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Testosterone (Testo) and unlabeled Testosterone (Testo) (Standards or samples) with the pre-coated antibody specific to Testosterone (Testo). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Testosterone (Testo) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Testosterone (Testo) in the sample.

Citations

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  • Insulin Receptor Substrate 2 Is Required for Testicular DevelopmentPubMed: PMC3669358
  • Central effects of camphor on GnRH and sexual hormones in male ratPubmed: 24551777
  • Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells Pubmed: 23954356
  • Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in RatsPubmed: 24051982
  • The effect of sertraline, paroxetine, fluoxetine and escitalopram on testicular tissue and oxidative stress parameters in rats.Pubmed: 24642156
  • Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineeringRsc: Source
  • Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesisScienceDirect: S0006291X14001843
  • Diets rich in saturated and polyunsaturated fatty acids induce morphological alterations in the rat ventral prostatePubmed:Pmc4100933
  • Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspringPubmed:24126190
  • The impact of maternal consumption of cafeteria diet on reproductive function in the offspringPubmed:24631302
  • The effect of Sertraline, Paroxetine, Fluoxetine and Escitalopram on testicular tissue and oxidative stress parameters in ratsPubmed:24642156
  • Perfluorooctane sulfonate effects on the reproductive axis in adult male ratsPubmed:25171141
  • Lard and/or canola oil-rich diets induce penile morphological alterations in a rat model.Pubmed:25185055
  • Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertilityPubmed:25505846
  • Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapyTurkishjournalofurology:Source
  • Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetesPubMed: 26379831
  • Metabolic and Testicular Effects of the Long-Term Administration of Different High-Fat Diets in Adult RatsPubMed: 26200553
  • Plasma Testosterone and Testis Histological Features of Mice Treated with Averrhoa Bilimbi, Cosmos Caudatus and Pereskia Bleo Ethanolic ExtractAensiweb: Aeb
  • Elevated Serum Bisphenol A Level in Patients with Dilated CardiomyopathyPubMed: 25996886
  • Effect on the H20 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis periodPubMed: 25782374
  • Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarctionPubMed: 25770118
  • Preparation and evaluation of a phospholipid-based injectable gel for the long term delivery of leuprolide acetaterrhscience:S2211383516301812
  • Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t MicrobiotaPubmed:27093642
  • Expression of androgen-producing enzyme genes and testosterone concentration in AnCavia (Guinea pig )s and Nellore heifers with high and low ovarian follicle countPubmed:26948295
  • Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARαPubmed:27413362
  • THE EFFECT OF KISSPEPTIN ON SPERMATOGENESIS AND APOPTOSIS IN RATSfileID:693211
  • Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in MicePubmed:26771609
  • Molecular characterization of insulin resistance and glycolytic metabolism in the rat uteruspubmed:27461373
  • Maternal Sleep Deprivation Alters Reproductive Capability of Male Offspring in Wistar RatsAkindele922016JALSI29782.pdf
  • Recovery of fertility in quinestrol-treated or diethylstilbestrol-treated mice: Implications for rodent management.pubmed:27611741
  • Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.pubmed:28336389
  • Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum.pubmed:27838061
  • Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARαpubmed:27413362
  • Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats.pubmed:28000943
  • The effect of kisspeptin on spermatogenesis and apoptosis in rats.pubmed:28263511
  • The Angiogenic Effect of Testosterone Supplementation on Brain of Aged Mice.13006045
  • Effect of dietary fish oil on mouse testosterone level and the distribution of eicosapentaenoic acid-containing phosphatidylcholine in testicular interstitiumS2405580816300991
  • Abnormality of Klotho Signaling Is Involved in Polycystic Ovary Syndromepubmed:28673204
  • Toxic effects of Tripterygium wilfordii Hook F on the reproductive system of adolescent male ratspubmed:28938525
  • Testosterone deficiency prevents left ventricular contractility dysfunction after myocardial infarction pubmed:28606867
  • The effect of testosterone supplementation on morphometry and VEGF expression level of thoracic aorta in aged miceDOI: 10.1501/Vetfak_0000002791
  • Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob -/-)pubmed:28506466
  • Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathwaypubmed:29020999
  • Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island populationPubmed:29525625
  • Exposure to tris (1, 3-dichloro-2-propyl) phosphate for Two generations decreases fecundity of zebrafish at environmentally relevant concentrationsPubmed:29775925
  • deficiency leads to testicular dysfunction and altered expression of genes involved in cell communication, chromatin reorganization, spermatogenesis, and …Pubmed:29411926
  • DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathwayPubmed:29321035
  • Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) …Pubmed:29627845
  • Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes …10.3892:ol.2018.9165
  • Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neuronsDoi: 10.1016/j.biopha.2018.08.135
  • Resveratrol attenuates metabolic, sperm, and testicular changes in adult Wistar rats fed a diet rich in lipids and simple carbohydratesPubmed: 30198494
  • 基因敲除损伤小鼠睾丸睾酮产生与生精功能
  • Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in RatsPubmed: 30569668
  • Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients
  • Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndromePubmed: 29535146
  • Brain‐derived neurotrophic factor: A steroidogenic regulator of Leydig cellsPubmed: 30628054
  • Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary
  • iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine
  • The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study.
  • The Effect of Testosterone Supplementation on Capillar Morphometry and VEGF Expression Level of the Heart in Aged Mice
  • Cyclophilin D participates in the inhibitory effect of high‐fat diet on the expression of steroidogenic acute regulatory proteinPubmed: 31373170
  • Revisiting the relationships of 2D:4D with androgen receptor (AR) gene and current testosterone levels: Replication study and meta‐analysesPubmed: 31359506
  • Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male ratsPubmed: 31353623
  • Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signalingPubmed: 31809938
  • Effects of fast food packaging plasticizers and their metabolites on steroid hormone synthesis in H295R cellsPubmed: 32334352
  • Serum Progesterone and Testosterone Levels in Schizophrenia Patients at Different Stages of TreatmentPubmed: 33159671
  • Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance …Pubmed: 33109277
  • Novel antihypertensive action of rutin is mediated via inhibition of angiotensin converting enzyme/mineralocorticoid receptor/angiotensin 2 type 1 receptor (ATR1) …Pubmed: 33089540
  • Parental exposure to polystyrene microplastics at environmentally relevant concentrations has negligible transgenerational effects on zebrafish (Danio rerio)Pubmed: 33011509
  • MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistancePubmed: 32757043
  • Electroacupuncture alleviates polycystic ovary syndrome-like symptoms through improving insulin resistance, mitochondrial dysfunction, and endoplasmic …Pubmed: 32698821
  • Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal ModelPubmed: 32517356
  • Maternal CBZ exposure impairs testicular development, spermatogenesis and sperm parameters in male offspring at pubertyPubmed: 32478927
  • N, N-Dimethylacetamide, an FDA approved excipient, acts post-meiotically to impair spermatogenesis and cause infertility in ratsPubmed: 32447106
  • Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat StressPubmed: 32685488
  • Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS
  • IVERMECTINA IMPEDE DANOS TESTICULARES EM RATOS
  • The effects of gold nanoparticles on Leydig cells and male reproductive function in mice
  • The Effect of Acupuncture on MiRNA Expression Profiles in the Ovaries of Rats with PCOS and Insulin Resistance
  • BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-¦ÊB pathways in aging mice induced by D-galactose33508368
  • Exposure to the bromodomain inhibitor N-methyl pyrrolidone blocks spermatogenesis in a hormonal and non-hormonal fashion33965371
  • A comparative study on the aphrodisiac activity of food plants< i> Mondia whitei, Chenopodium album, Ccucurbita pe...
  • MiR-1224-5p attenuates polycystic ovary syndrome through inhibiting NOD-like receptor protein 3 inflammasome activation via targeting Forkhead box O 134637688
  • Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice34730391
  • Yohimbine hydrochloride inhibits benign prostatic hyperplasia by downregulating steroid 5α-reductase type 234265299
  • Superior effect of broccoli methanolic extract on control of oxidative damage of sperm cryopreservation and reproductive performance in rats: A comparison with …Pubmed:35063921
  • Cylindrospermopsin Disrupts Estrous Cycle and Increases Spermatogenesis in MicePubmed:35257354

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