ELISA Kit for Troponin T Type 2, Cardiac (TNNT2)Homo sapiens (Human)Sandwich ELISA

CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T

  • Product No.SED232Hu
  • Organism SpeciesHomo sapiens (Human)
  • Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range15.62-1000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 6.1pg/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Troponin T Type 2, Cardiac (TNNT2).
    No significant cross-reactivity or interference between Troponin T Type 2, Cardiac (TNNT2) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection
  • DownloadInstruction Manual
  • Format48T96T96T*596T*1096T*100
  • FOBUS$ 490US$ 700US$ 3150US$ 5950US$ 49000
  • ELISA Kit for Troponin T Type 2, Cardiac (TNNT2)Package and Components
  • ELISA Kit for Troponin T Type 2, Cardiac (TNNT2)Package and Components
  • ELISA Kit for Troponin T Type 2, Cardiac (TNNT2)Results demonstration
  • SED232Hu.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Troponin T Type 2, Cardiac (TNNT2) and the recovery rates were calculated by comparing the measured value to the expected amount of Troponin T Type 2, Cardiac (TNNT2) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)93-105101
EDTA plasma(n=5)78-9881
heparin plasma(n=5)93-10197

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Troponin T Type 2, Cardiac (TNNT2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Troponin T Type 2, Cardiac (TNNT2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Troponin T Type 2, Cardiac (TNNT2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)88-96%90-101%87-99%84-99%
EDTA plasma(n=5)80-93%81-91%86-101%95-105%
heparin plasma(n=5)98-105%80-92%92-104%79-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Troponin T Type 2, Cardiac (TNNT2)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin T Type 2, Cardiac (TNNT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Troponin T Type 2, Cardiac (TNNT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin T Type 2, Cardiac (TNNT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin T Type 2, Cardiac (TNNT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reference

  • Basic & Clinical Pharmacology & ToxicologyCardioprotective Effect of Sulphonated Formononetin on Acute Myocardial Infarction in RatsPubMed: 21232020
  • Basic & Clinical Pharmacology & ToxicologyCardioprotective Effect of SMND-309, A Novel Derivate of Salvianolic Acid B on Acute Myocardial Infarction in RatsWiley: source
  • European Journal of PharmacologyProtective roles of Asperosaponin VI, a triterpene saponin isolated from Dipsacus asper Wall on acute myocardial infarction in ratsScienceDirect: S0014299909009984
  • Journal of translational medicineThe altered expression profile of microRNAs in cardiopulmonary bypass canine models and the effects of mir-499 on myocardial ischemic reperfusion injuryPubMed: PMC3694448
  • Oral Diseases Salivary high‐sensitivity cardiac troponin T levels in patients with acute myocardial infarctionPubmed: 22834943
  • An International Journal of Pharmaceutical SciencesIn vivo and in vitro cardioprotective effects of Panax quinquefolium 20 (S)-protopanaxadiol saponins (PQDS), isolated from Panax quinquefoliumPubmed: 23700796
  • Journal of Animal and Feed Sciences,The effects of caloric restriction and age on thyroid hormone signalling in the heart of ratsPl:Source
  • Liver Int.Cardiomyopathy reverses with recovery of liver injury, cholestasis and cholanemia in mouse model of biliary fibrosisPubmed:24330504
  • Cardiovasc Toxicol. Cardioprotective Effect of Rhizomes of Acorus gramineus Against Isoproterenol-Induced Cardiac Damage in PigsPubmed:24420420
  • British Journal of PharmacologyGlutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in ageing diabetic mice hearts during exercisePubmed:25039894