ELISA Kit for Troponin T Type 2, Cardiac (TNNT2) Homo sapiens (Human) Sandwich ELISA

CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T

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  • ELISA Kit for Troponin T Type 2, Cardiac (TNNT2) Packages (Simulation)
  • ELISA Kit for Troponin T Type 2, Cardiac (TNNT2) Packages (Simulation)
  • ELISA Kit for Troponin T Type 2, Cardiac (TNNT2) Results demonstration
  • SED232Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Troponin T Type 2, Cardiac (TNNT2) and the recovery rates were calculated by comparing the measured value to the expected amount of Troponin T Type 2, Cardiac (TNNT2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-105 101
EDTA plasma(n=5) 78-98 81
heparin plasma(n=5) 93-101 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Troponin T Type 2, Cardiac (TNNT2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Troponin T Type 2, Cardiac (TNNT2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Troponin T Type 2, Cardiac (TNNT2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-96% 90-101% 87-99% 84-99%
EDTA plasma(n=5) 80-93% 81-91% 86-101% 95-105%
heparin plasma(n=5) 98-105% 80-92% 92-104% 79-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Troponin T Type 2, Cardiac (TNNT2)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin T Type 2, Cardiac (TNNT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Troponin T Type 2, Cardiac (TNNT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin T Type 2, Cardiac (TNNT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin T Type 2, Cardiac (TNNT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Cardioprotective Effect of Sulphonated Formononetin on Acute Myocardial Infarction in RatsPubMed: 21232020
  • Cardioprotective Effect of SMND-309, A Novel Derivate of Salvianolic Acid B on Acute Myocardial Infarction in RatsWiley: source
  • Protective roles of Asperosaponin VI, a triterpene saponin isolated from Dipsacus asper Wall on acute myocardial infarction in ratsScienceDirect: S0014299909009984
  • The altered expression profile of microRNAs in cardiopulmonary bypass canine models and the effects of mir-499 on myocardial ischemic reperfusion injuryPubMed: PMC3694448
  • Salivary high‐sensitivity cardiac troponin T levels in patients with acute myocardial infarctionPubmed: 22834943
  • In vivo and in vitro cardioprotective effects of Panax quinquefolium 20 (S)-protopanaxadiol saponins (PQDS), isolated from Panax quinquefoliumPubmed: 23700796
  • The effects of caloric restriction and age on thyroid hormone signalling in the heart of ratsPl:Source
  • Cardiomyopathy reverses with recovery of liver injury, cholestasis and cholanemia in mouse model of biliary fibrosisPubmed:24330504
  • Cardioprotective Effect of Rhizomes of Acorus gramineus Against Isoproterenol-Induced Cardiac Damage in PigsPubmed:24420420
  • Glutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in ageing diabetic mice hearts during exercisePubmed:25039894
  • Crocin protects against doxorubicin-induced myocardial toxicity in rats through down-reCavia (Guinea pig )lation of inflammatory and apoptic pathwaysPubmed:26807765
  • The Cardenolide Glycoside Acovenoside A Affords Protective Activity in Doxorubicin-Induced Cardiotoxicity in MicePubmed:27247000
  • Troponins, heat shock proteins and glycogen phosphorylase BB in umbilical cord blood of complicated pregnancies.pubmed:27937003
  • Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice.pubmed:27907155
  • Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in ratspubmed:28076830
  • Imatinib attenuates cardiac fibrosis by inhibiting platelet-derived growth factor receptors activation in isoproterenol induced modelpubmed:28570599
  • Cardioprotective effect of Notch signaling on the development of myocardial infarction complicated by diabetes mellituspubmed:29042932
  • Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injuryPubmed: 30192780
  • Tissue suction-mediated gene transfer to the beating heart in micePubmed: 32027678
  • Hormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle HypertrophyPubmed: 32148580
  • Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stressPubmed: 33022535
  • Norepinephrine Leads to More Cardiopulmonary Toxicities than Epinephrine by Catecholamine Overdose in RatsPubmed: 32947820
  • Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice33526170
  • Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species34536344
  • Development of a novel ssDNA aptamer targeting cardiac troponin I and its clinical applications34673993
  • Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrestPubmed:35495584

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