Instant ELISA Kit for Interleukin 8 (IL8) Cavia (Guinea pig ) Instant ELISA

CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1

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  • Instant ELISA Kit for Interleukin 8 (IL8) Packages (Simulation)
  • Instant ELISA Kit for Interleukin 8 (IL8) Packages (Simulation)
  • Instant ELISA Kit for Interleukin 8 (IL8) Results demonstration
  • IEA080Gu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Instant Interleukin 8 (IL8) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Interleukin 8 (IL8) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-96 84
EDTA plasma(n=5) 78-101 83
heparin plasma(n=5) 82-90 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Interleukin 8 (IL8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Interleukin 8 (IL8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Interleukin 8 (IL8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-90% 94-102% 95-102% 94-105%
EDTA plasma(n=5) 92-101% 93-101% 84-94% 96-105%
heparin plasma(n=5) 90-98% 90-99% 86-95% 92-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 5 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 30 minutes at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 30 minutes at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Instant ELISA Kit for Interleukin 8 (IL8)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Interleukin 8 (IL8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Instant Interleukin 8 (IL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Instant Interleukin 8 (IL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Interleukin 8 (IL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Evidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cowRbej: 14777827
  • Serial cytokine levels during wound healing in rabbit maxillary sinus mucosaPubMed: 19958244
  • Experimental peri-implant mucositis at different implant surfaces.Pubmed: 24521508
  • Budesonide added to modified porcine surfactant Curosurf may additionally improve the lung functions in meconium aspiration syndrome.Pubmed: 2432969
  • Serum levels of selected chemokines in systemic lupus erythematosus patientsPubmed: 22461186
  • Expression of interleukine-8 as an independent prognostic factor for sporadic colon cancer disseminationPubmed:Pmc4197484
  • Experimental peri‐implant mucositis at different implant surfacesPubmed:24521508
  • Grape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental?×?Qinchuan steersPubmed:24846452
  • Inflammatory cytokine concentrations in uterine flush and serum samples from dairy cows with clinical or subclinical endometritisTheriojournal:Source
  • Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranesPubMed: 25851495
  • ДИНАМИКА ЦИТОКИНОВОГО ПРОФИЛЯ В ОЦЕНКЕ ЭФФЕКТИВНОСТИ РАЗНЫХ МЕТОДОВ СТИМУЛЯЦИИ РЕПАРАТИВНОГО ОСТЕОГЕНЕЗА В ЭКСПЕРИМЕНТЕArticle: N
  • Hyperinflation deteriorates arterial oxygenation and lung injury in a rabbit model of ARDS with repeated open endotracheal suctioningPubMed: 25943099
  • Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgeryPubMed: 25719610
  • The Predictive Diagnostic and Prognostic Cut-off Values for Interleukin 8 in Patients with Meningitis in EgyptProfile: Mohmad_Mosaad
  • Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation periodPubmed:27090626
  • Infection of chicken bone marrow mononuclear cells with subgroup J avian leukosis virus inhibits dendritic cell differentiation and alters cytokine expressionPubmed:27349993
  • Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injuryscience:S1995764516300955
  • Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrolpublication:299492265_Diabetes-induced_renal_failu
  • Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells10610
  • Mutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease.pubmed:28088561
  • Intravenous dexamethasone attenuated inflammation and influenced apoptosis of lung cells in an experimental model of acute lung injury.pubmed:28006948
  • Mechanical Ventilation Alters the Development of Staphylococcus aureus Pneumonia in Rabbit.pubmed:27391952
  • Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung Injuryacm.2017.16.issue-3
  • Effects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavagemodel of acute lung injury.pubmed:28195073
  • 小剂量阿奇霉素对稳定期老年慢性阻塞性肺疾病患者外周血内TNF-α、IL-8、CRP水平及肺功能的影响Periodical_zgywjj201608004
  • Cytokine levels in colostrum and in foals' serum pre- and post-sucklingpubmed:28242000
  • Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Modelspubmed:27739057
  • Cardioprotective time-window of Penehyclidine hydrochloride postconditioning: A rat studypubmed:28684235
  • Linezolid and atorvastatin impact on pneumonia caused by Staphyloccocus aureus in rabbits with or without mechanical ventilationpubmed:29149185
  • The effect of restorative materials on cytokines in gingival crevicular fluidpubmed:28992599
  • Experimentelle periimplantäre Mukositis an unterschiedlichen Implantatoberflächendoi: 10.1111/jcpe.12240.
  • CagA promotes proliferation and inhibits apoptosis of GES-1 cells by upregulating TRAF1/4-1BBpubmed:28627614
  • Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosispubmed:28608724
  • Alteration in some pro and anti-inflammatory cytokines associated with complete and incomplete gestation cycle of cows10.1080/09291016.2017.1319636
  • Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheetspubmed:28811665
  • Inhibitory Effect of Low-Intensity Pulsed Ultrasound on the Expression of Lipopolysaccharide-Induced Inflammatory Factors in U937 Cells.pubmed:28600899
  • Integrated effect of seasons and lactation stages on the plasma inflammatory cytokines, function and receptor expression of milk neutrophils in Sahiwal (Bos indicus) cows.pubmed:28895861
  • Comparison of Polymyxin E and Polymyxin B as an Additive to Pulmonary Surfactant in Escherichia coli Pneumonia of Ventilated Neonatal Rabbits10.1159/000475877
  • Association Between Endothelial Progenitor Cells and Treatment Response in Non-Squamous Non-small Cell Lung Cancer Treated with Bevacizumab.pubmed:28982871
  • Inflammatory biomarkers and radiologic measurements in never-smokers with COPD: A cross-sectional study from the CODA cohort.pubmed:29117798
  • Polar extract of protects cartilage homeostasis: possible mechanism of actionPubmed:29313174
  • Dexmedetomidine attenuates renal fibrosis via α2-adrenergic receptor-dependent inhibition of cellular senescence after renal ischemia/reperfusionPubmed:29729264
  • Pathogen-dependent modulation of milk neutrophils competence, plasma inflammatory cytokines and milk quality during intramammary infection of Sahiwal (Bos …Pubmed:29787791
  • Evaluation of the therapeutic effect of micro-plasma radio frequency on hypertrophic scars in rabbit earsPubmed:30003425
  • Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4) …Pubmed:29804125
  • Rifaximin anti‐inflammatory activity on bovine endometrium primary cell cultures: a preliminary studyPubmed:29984902
  • N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation.Doi: 10.1042/BSR20180858
  • Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung …Doi: 10.1515/acm-2016-0012
  • Metabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cowsPubmed: 17430930
  • Interleukin 8 targeted contrast echocardiography is effective to evaluate myocardial ischemia-reperfusion injury in the rabbits
  • Immunogenicity and protective efficacy against Treponema pallidum in New Zealand rabbits immunized with plasmid DNA encoding flagellinPubmed: 30405111
  • The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung diseasePubmed: 30573494
  • Altered serum levels of type I collagen turnover indicators accompanied by IL-6 and IL-8 release in stable COPDPubmed: 30655663
  • Chandrasekaran Chinampudur Velusami, Edwin Jothie Richard &
  • Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment …Pubmed: 30621731
  • Effective Treatment of Bovine Mastitis with Intramammary Infusion of Angelica dahurica and Rheum officinale Extracts
  • Naoxintong Capsule Inhibits the Development of Cardiovascular Pathological Changes in Bama Minipig Through Improving Gut MicrobiotaPubmed: 31632272
  • Anti-Inflammatory and Anti-Apoptotic Effects of Stybenpropol A on Human Umbilical Vein Endothelial CellsPubmed: 31671764
  • Corrosion Resistance of Graphene oxide/Silver Coatings on Ni–Ti alloy and Expression of IL-6 and IL-8 in Human Oral FibroblastsPubmed: 32094428
  • Suppressor of ras val-2 promotes inflammation-mediated oxidative stress and cell apoptosis in cardiomyocytes through activating Mst1-mROS signaling pathwayPubmed: 32065019
  • Effects of Early Intervention with Antibiotics and Maternal Fecal Microbiota on Transcriptomic Profiling Ileal Mucusa in Neonatal PigsPubmed: 31963653
  • Effect of Different Dosages of Dexamethasone Therapy on Lung Function and Inflammation in an Early Phase of Acute Respiratory Distress Syndrome ModelPubmed: 31928043
  • Synthetic surfactant with a recombinant surfactant protein C analogue improves lung function and attenuates inflammation in a model of acute respiratory …Pubmed: 31694668
  • Tectorigenin inhibits inflammation and pulmonary fibrosis in allergic asthma model of ovalbumin‐sensitized guinea pigsPubmed: 32314371
  • Cyanidin-3-O-Glucoside Attenuates Lipopolysaccharide-Induced Inflammation in Human Corneal Epithelial Cells by Inducing Let-7b-5p-Mediated HMGA2/PI3K/Akt …Pubmed: 32248330
  • NFKB1 gene rs28362491 ins/del variation is associated with higher susceptibility to myocardial infarction in a Chinese Han populationPubmed: 33177541
  • Study on Adenovirus Infection in vitro with Nanoself-Assembling Peptide as Scaffolds for 3D CulturePubmed: 32922004
  • A comparative study on various immunological parameters influencing embryo survivability in crossbred dairy cowsPubmed: 32810791
  • SIRT1 relieves Necrotizing Enterocolitis through inactivation of Hypoxia-inducible factor (HIF)-1aPubmed: 32657204
  • Hyperoside Protects Human Umbilical Vein Endothelial Cells Against Anticardiolipin Antibody-Induced Injury by Activating AutophagyPubmed: 32508661
  • Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy …
  • Diet Supplementation with a Bioactive Pomace Extract from Olea europaea Partially Mitigates Negative Effects on Gut Health Arising from a Short-Term Fasting Period …
  • Possible Anti-inflammatory Effects of Mesenchymal Stem Cells Transplantation via Changes in CXCL8 Levels in Patients with Refractory Rheumatoid Arthritis
  • HMGB1 e citocinas inflamatórias em lesão pulmonar aguda experimental induzida em coelhos
  • Inflammation Mediators Regulate the Microbiota Resistance to Adverse Factors33222085
  • Impact of synthetic surfactant CHF5633 with SP©\B and SP©\C analogues on lung function and inflammation in rabbit model of acute respiratory distress syndrome33403805
  • Cough Inhibition Activity of Schisandra chinensis in Guinea Pigs33861937
  • Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population33850223
  • Corrosion Resistance of Graphene oxide/Silver Coatings on Ni¨CTi alloy and Expression of IL-6 and IL-8 in Human O...32094428
  • Response of broiler chickens fed diets supplemented with a bioactive olive pomace extract from Olea europaea to an experimental coccidial vaccine?¡­33518110
  • Construction of heparin-based hydrogel incorporated with Cu5. 4O ultrasmall nanozymes for wound healing and inflammation inhibition33778192
  • Immunohistochemical Expression of Neurokinin-A and Interleukin-8 in the Bronchial Epithelium of Horses with Severe Equine Asthma Syndrome during Asymptomatic?¡­34066204
  • Talaromyces marneffei and nontuberculous mycobacteria co-infection in HIV-negative patients34376749
  • Nitric-Oxide-Releasing Dexamethasone Derivative NCX-1005 Improves Lung Function and Attenuates Inflammation in Experimental Lavage-Induced ARDS34959373
  • Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes34829910
  • Adverse Mechanical Ventilation and Pneumococcal Pneumonia Induce Immune and Mitochondrial Dysfunctions Mitigated by Mesenchymal Stem Cells in RabbitsPubmed:34965287
  • Endometrial Dysbiosis Is Related to Inflammatory Factors in Women with Repeated Implantation Failure: A Pilot StudyPubmed:35566605
  • Cell damage and neutrophils promote the infection of Mycoplasma pneumoniae and inflammatory responsePubmed:35724831
  • LncRNA KCNQ10T1 shuttled by bone marrow mesenchymal stem cells-derived exosome inhibits sepsis via regulation of miR-154-3p/RNF19A axis
  • Anti-inflammatory activity of non-selective PDE inhibitor aminophylline on the lung tissue and respiratory parameters in animal model of ARDS
  • Evaluating the Roles of sCD14 and sCD14-ST in Diagnosing COPD and Predicting an Acute Exacerbation of COPD

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