Mini Samples ELISA Kit for Interleukin 12 (IL12) Mus musculus (Mouse) Mini ELISA

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  • Mini Samples ELISA Kit for Interleukin 12 (IL12) Packages (Simulation)
  • Mini Samples ELISA Kit for Interleukin 12 (IL12) Packages (Simulation)
  • Mini Samples ELISA Kit for Interleukin 12 (IL12) Results demonstration
  • MEA111Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Interleukin 12 (IL12) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Interleukin 12 (IL12) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-94 91
EDTA plasma(n=5) 91-103 97
heparin plasma(n=5) 95-103 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 12 (IL12) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 12 (IL12) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Interleukin 12 (IL12) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-101% 95-102% 80-103% 84-91%
EDTA plasma(n=5) 87-101% 88-96% 93-105% 85-93%
heparin plasma(n=5) 79-98% 79-95% 94-102% 78-90%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

Mini Samples ELISA Kit for Interleukin 12 (IL12)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Interleukin 12 (IL12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Interleukin 12 (IL12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Interleukin 12 (IL12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Interleukin 12 (IL12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys.pubmed:
  • A Novel Potential Strategy for Enhancing Antigen Presentation of Human Hepatocellular Carcinoma Cells Propagated ex-vivo Using a Ribonuclease Enzyme …Scholarena:Source
  • Switched memory B cells promote alveolar bone damage during periodontitis: An adoptive transfer experimentPubmed:30015235
  • Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitroPubmed:30127867
  • The P2X7 receptor, cathepsin S and fractalkine in the trigeminal subnucleus caudalis signal persistent hypernociception in temporomandibular rat jointsPubmed: 30248434
  • Soluble LAG3 acts as a potential prognostic marker of gastric cancer and its positive correlation with CD8+ T cell frequency and secretion of IL-12 and INF-γ in …Pubmed: 30223387
  • The relationship between autophagy and apoptosis of lamina propria dendritic cells from the small intestine on the immunodynamics of sepsis mice
  • ВЛИЯНИЕ ДНК МЕТИЛТРАНСФЕРАЗЫ 3А И ГИСТОНДЕАЦЕТИЛАЗЫ НА СОДЕРЖАНИЕ В МОНОНУКЛЕАРНЫХ КЛЕТКАХ ЦЕЛЬНОЙ КРОВИ …
  • RBP and Alb in neonatal hydronephrosis and its association with IL-12 levels in pregnant women before delivery
  • Atmospheric H2S triggers immune damage by activating the TLR-7/MyD88/NF-κB pathway and NLRP3 inflammasome in broiler thymus
  • Disseminated And Infection Accompanied Sweet's Syndrome In A Patient With Anti-Interferon-γ Autoantibodies: A Case Report.Pubmed: 31632104
  • Suppression of Fpr2 expression protects against endotoxin-induced acute lung injury by interacting with Nrf2-regulated TAK1 activationPubmed: 32106380
  • Silkworm pupa oil attenuates acetaminophen‐induced acute liver injury by inhibiting oxidative stress‐mediated NF‐κB signalingPubmed: 31993149
  • Innate immune response of mammary gland induced by intramammary infusion of Bifidobacterium breve in lactating dairy cowsPubmed: 33071254
  • Vasküler Demans Modelinde Çevresel Zenginleştirmenin Depresyon Benzeri Davranış, Kortikal ve Hipokampal BDNF ve IL-1β Seviyeleri Üzerine Etkisi
  • Evaluation of lipopolysaccharide-induced acute lung injury attenuation in mice by Glycyrrhiza glabra
  • BBG enhances OLT1177-induced NLRP3 inflammasome inactivation by targeting P2X7R/NLRP3 and MyD88/NF-¦ÊB signaling in DSS-induced colitis in rats33548287
  • HOXA5 induces M2 macrophage polarization to attenuate carotid atherosclerosis by activating MED134117711
  • Establishing novel roles of bifidocin LHA, antibacterial, antibiofilm and immunomodulator against Pseudomonas aeruginosa corneal infection model34171261
  • Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma34377237
  • Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study34730850
  • Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes34829910
  • Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBMPubmed:35351131
  • Cyclosporine A loaded brain targeting nanoparticle to treat cerebral ischemia/reperfusion injury in micePubmed:35658867
  • Deletion of Toxoplasma Rhoptry Protein 38 (PruΔrop38) as a Vaccine Candidate for Toxoplasmosis in a Murine ModelPubmed:35740356

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