Mini Samples ELISA Kit for Interleukin 9 (IL9) Rattus norvegicus (Rat) Mini ELISA

HP40; P40; TCGFIII; TCGF3; T Cell Growth Factor III; P40 T-Cell And Mast Cell Growth Factor; P40 Cytokine; Homolog Of Mouse T Cell And Mast Cell Growth Factor 40

  • Product No.MEA081Ra
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length3h
  • Detection Range3.12-200pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 1.36pg/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 9 (IL9).No significant cross-reactivity or interference between Mini Samples Interleukin 9 (IL9) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
  • DownloadInstruction Manual
  • UOM 48T96T 96T*5 96T*10 96T*100 In stock
  • FOB US$ 575.00 US$ 821.00 US$ 3,695.00 US$ 6,979.00 US$ 57,470.00
  • Quantity
  • Add to Cart Distributors
  • Mini Samples ELISA Kit for Interleukin 9 (IL9) Packages (Simulation)
  • Mini Samples ELISA Kit for Interleukin 9 (IL9) Packages (Simulation)
  • Mini Samples ELISA Kit for Interleukin 9 (IL9) Results demonstration
  • MEA081Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Interleukin 9 (IL9) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Interleukin 9 (IL9) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-92 88
EDTA plasma(n=5) 81-88 85
heparin plasma(n=5) 97-104 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 9 (IL9) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 9 (IL9) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Interleukin 9 (IL9) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-96% 86-101% 95-105% 81-95%
EDTA plasma(n=5) 88-102% 97-104% 91-98% 97-105%
heparin plasma(n=5) 84-97% 93-104% 88-97% 89-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

Mini Samples ELISA Kit for Interleukin 9 (IL9)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Interleukin 9 (IL9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Interleukin 9 (IL9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Interleukin 9 (IL9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Interleukin 9 (IL9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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