Active Arylsulfatase B (ARSB) Homo sapiens (Human) Active protein

ASB; G4S; N-acetylgalactosamine-4-sulfatase

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Overview
Properties
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 80%
  • Isoelectric Point7.7
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  • Active Arylsulfatase B (ARSB) Packages (Simulation)
  • Active Arylsulfatase B (ARSB) Packages (Simulation)
  • APG610Hu01.jpg Figure. SDS-PAGE
  • Active Arylsulfatase B (ARSB) WB Image
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family. ARSB hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ARSB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSB and recombinant human uPAR. Briefly, ARSB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ARSB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of ARSB and uPAR was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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