Active Erythropoietin (EPO) Homo sapiens (Human) Active protein

EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point7.0
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  • Active Erythropoietin (EPO) Packages (Simulation)
  • Active Erythropoietin (EPO) Packages (Simulation)
  • APA028Hu01.png Figure. SDS-PAGE
  • Active Erythropoietin (EPO) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Erythropoietin (EPO), also known as hematopoietin or hemopoietin, is a glycoprotein cytokine secreted by the kidney in response to cellular hypoxia; it stimulates red blood cell production (erythropoiesis) in the bone marrow. Erythropoietin is an essential hormone for red blood cell production. EPO can cooperate with various other growth factors involved in the development of erythroid lineage from multipotent progenitors. To test the effect of EPO on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 including various concentrations of recombinant human EPO. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of TF-1 cells after incubation with EPO for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant EPO for 72h. The result was shown in Figure 2. It was obvious that EPO significantly increased cell viability of TF-1 cells.
(A) TF-1 cells cultured in 1640, stimulated with 5ng/mL EPO for 72h;
(B) Unstimulated TF-1 cells cultured in1640 for 72h.
Figure. Cell proliferation of TF-1 cells after stimulated with EPO.

Figure. Cell proliferation of TF-1 cells after stimulated with EPO

Usage

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Prenatal Glucocorticoid Overexposure Causes Permanent Increases in Renal Erythropoietin Expression and Red Blood Cell Mass in the Rat OffspringEndo: source
  • Inhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expressionPubMed: 21333724
  • CD98 Positive Eosinophils Contribute to T Helper 1 Pattern InflammationPlosOne: Source
  • Alterations of Circulating Endothelial Cell and Endothelial Progenitor Cell Counts around the OvulationPubMed: 22948762
  • Long-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expressionPubMed: PMC3509184
  • Reevaluation of erythropoietin production by the nephronPubmed:24832733
  • Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m.Pubmed:2528040
  • Decreased plasma soluble erythropoietin receptor in high-altitude excessive erythrocytosis and Chronic Mountain SicknessPubmed:25324511
  • Plasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferronePubMed: 26496240
  • KIAA0101 is associated with human renal cell carcinoma proliferation and migration induced by erythropoietinpubmed:26575329
  • Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors.pubmed:28216608
  • Occult gastrointestinal bleeding is a common finding in dogs with chronic kidney diseasepubmed:28186645
  • An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's …articles:journal-of-alzheimers-disease
  • Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activationpubmed:29147463
  • Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosispubmed:28489854
  • Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on duration of anoxia S0197018617304783
  • Relation between anaemia and bone marrow features and serum erythropoietin in dogs with chronic kidney disease10.1590/s0100-736x2017000600011 
  • Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation10.1016/j.mjafi.2017.04.003
  • Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on …Pubmed:29180303
  • Increased NGAL level associated with iron store in chronic kidney disease with anemiaPubmed:29909502
  • Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate HypoxiaPubmed:29308565
  • Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in …Pubmed: 30353332
  • High-altitude hypoxia decreases plasma erythropoietin soluble receptor (sEpoR) concentration in humans
  • Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats
  • PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE
  • Effects of carbohydrate and glutamine supplementation on cytokine production by monocytes after exercise in hypoxia: a crossover, randomized and double-blind …Pubmed: 31743809
  • Disordered serum erythroferrone and hepcidin levels as indicators of the spontaneous abortion occurrence during early pregnancy in humansPubmed: 32866306
  • Increased FGF-23 levels are linked to ineffective erythropoiesis and impaired bone mineralization in myelodysplastic syndromesPubmed: 32759495
  • EPAS1 regulates proliferation of erythroblasts in chronic mountain sicknessPubmed: 32470757
  • miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia34367277
  • Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 moleculesPubmed:35072831
  • Effect of arsenate on erythropoietin production and autophagy induction in HepG2 cells

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