Active Lecithin Cholesterol Acyltransferase (LCAT)Mus musculus (Mouse)Active protein

phosphatidylcholine-sterol O-acyltransferase; Phospholipid-cholesterol acyltransferase

  • Active Lecithin Cholesterol Acyltransferase (LCAT)Packages (Simulation)
  • Active Lecithin Cholesterol Acyltransferase (LCAT)Packages (Simulation)
  • APJ516Mu01.jpgFigure. SDS-PAGE; Sample: Active recombinant LCAT, Mouse.
  • Active Lecithin Cholesterol Acyltransferase (LCAT)Figure. Western Blot; Sample: Recombinant LCAT, Mouse.
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST

LCAT (Phosphatidylcholine-sterol acyltransferase) is an enzyme in the extracellular metabolism of plasma lipoproteins, which converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines. It is reported that APOA1 (Apolipoprotein A-I) acts as a cofactor for the LCAT. Thus, a binding ELISA assay was conducted to detect the association of LCAT with APOA1. Briefly, recombinant rat LCAT were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to APOA1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-LCAT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of LCAT with APOA1 was shown in Figure 1 and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

    Reference

    • Int J Rheum DisPlasma phospholipase, γ‐CEHC and antioxidant capacity in fibromyalgiaPubMed: 26585319