Active Lysyl Oxidase (LOX) Homo sapiens (Human) Active protein

Protein-Lysine 6-Oxidase

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Overview
Properties
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point6.2
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  • Active Lysyl Oxidase (LOX) Packages (Simulation)
  • Active Lysyl Oxidase (LOX) Packages (Simulation)
  • Active Lysyl Oxidase (LOX) Figure . Gene Sequencing (extract)
  • APC580Hu01.jpg SDS-PAGE
  • Active Lysyl Oxidase (LOX) Figure. Western Blot; Sample: Recombinant LOX, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Activity test

Lysyl oxidase (LOX) also known as protein-lysine 6-oxidase is an extracellular copper-dependent enzyme that catalyzes formation of aldehydes from lysine residues in collagen and elastin precursors. Its catalytic activity depends upon both its copper cofactor and a unique carbonyl cofactor and has been shown to extend to a variety of basic globular proteins, including histone H1. LOX plays a major role in connective tissue development and may also be important in neurological function. Lysyl oxidase has also proven crucial to the development of the respiratory system and the skin, the commitment step of adipocyte, and the formation of pluripotent stem cells during development. Its absence may lead to defects in the transforming growth factor beta superfamily of proteins, which control cell growth and differentiation. Besides, Elastin (ELN) has been identified as an interactor of LOX, thus a binding ELISA assay was conducted to detect the interaction of recombinant human LOX and recombinant human ELN. Briefly, LOX were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ELN-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-LOX pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of LOX and ELN was shown in Figure 1, and this effect was in a dose dependent manner.

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • The expression of lysyl-oxidase gene family members in myeloproliferative neoplasms.PubMed: 23494965
  • High Glucose Induced Differential Expression of Lysyl Oxidase and Its Isoform in ARPE-19 CellsPubmed: 22966818
  • Evaluation of serum lysyl oxidase as a blood test for colorectal cancer Pubmed: 24246612
  • Copia autorizada por CDRIngentaconnect: Source
  • Lysyl oxidase is a potential biomarker of fibrosis in systemic sclerosisPubmed: 24574233
  • Evaluation of serum lysyl oxidase as a blood test for colorectal cancerPubmed:Pmc4237001
  • Ionizing radiation induces tumor cell lysyl oxidase secretionPubmed:Pmc4223762
  • Lysyl Oxidase Genetic Variants Affect Gene Expression in Cervical CancerPubmed:24945327
  • Increased Serum Levels of the IL-33 Neutralizing sST2 in Limited Cutaneous Systemic SclerosisPubMed: 26095613
  • Correlation of Aqueous Humor Lysyl Oxidase Activity with TGF-ß Levels and LOXL1 Genotype in PseudoexfoliationPubmed:27116380
  • LOX Fails to Substitute for RANKL in Osteoclastogenesis.pubmed:27606829
  • Blocking Surgically Induced Lysyl Oxidase Activity Reduces the Risk of Lung Metastases.pubmed:28445728
  • Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen‑induced arthritispubmed:28901438
  • A Global Assessment of Circulating Prolysyl Oxidase in Nonischemic Patients With Garden-variety Heart Failure With Preserved Ejection FractionPubmed:29807761
  • Lysyl oxidase inhibition via β-aminoproprionitrile hampers human umbilical vein endothelial cell angiogenesis and migration in vitroPubmed:29393489
  • Lysyl Oxidase is a key player in BRAF/MAPK pathway-driven thyroid cancer aggressivenessPubmed: 30398411
  • Lysyl Oxidase Directly Contributes to Extracellular Matrix Production and Fibrosis in Systemic Sclerosis.Pubmed: 33026236
  • Collagen Cross©\Linking Is Associated With Cardiac Remodeling in Hypertrophic Obstructive Cardiomyopathy33356379

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