Recombinant Atrial Natriuretic Peptide (ANP) Homo sapiens (Human) Recombinant protein

ANH; ANF; CDD; Atrial Natriuretic Factor; Atrial Natriuretic Hormone; Atriopeptin; Cardionatrine; Cardiodilatin

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Molecular Mass 42.0kDa, Accurate 48kDa(Analysis of differences refer to the manual)
  • Residues & TagsLys41~Tyr151 with N-terminal His and GST Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point5.9
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  • Recombinant Atrial Natriuretic Peptide (ANP) Packages (Simulation)
  • Recombinant Atrial Natriuretic Peptide (ANP) Packages (Simulation)
  • Recombinant Atrial Natriuretic Peptide (ANP) Figure. Gene Sequencing (Extract)
  • RPA225Hu03.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Volume Status and Diuretic Therapy in Systolic Heart Failure and the Detection of Early Abnormalities in Renal and Tubular FunctionPubMed: 21616283
  • Release of atrial natriuretic peptide precedes shedding of the endothelial glycocalyx equally in patients undergoing on- and off-pump coronary artery bypass surgeryPubMed: 21769675
  • Atrial natriuretic Peptide, arginine vasopressin Peptide and cortisol serum levels in opiate-dependent patientsPubMed: 23406607
  • Alcohol-induced changes in methylation status of individual CpG sites, and serum levels of vasopressin and atrial natriuretic peptide in alcohol-dependent patients during detoxification treatment.Pubmed: 24356727
  • Hypervolemia increases release of atrial natriuretic peptide and shedding of the endothelial glycocalyxPubmed:25497357
  • Sodium-glucose co-transporter-2 inhibitors suppress atrial natriuretic peptide secretion in patients with newly diagnosed Type 2 diabetes.Pubmed:26927445
  • Danhong injection attenuates isoproterenol-induced cardiac hypertrophy by reCavia (Guinea pig )lating p38 and NF-κb pathwayPubmed:26970569
  • Blood Pressure and Atrial Natriuretic Peptide (ANP) Levels in Patients with Autosomal Dominant Polycystic Kidney Disease.papers:uobj_paper_2016_62613672.pdf
  • Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathypubmed:29240788
  • Ginsenoside Rg3 micelles mitigate doxorubicin-induced cardiotoxicity and enhance its anticancer efficacy.pubmed:29063791
  • SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylationPubmed:29504933
  • Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity andPubmed:29727991
  • Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in micePubmed:29929978
  • Determinants of Increased Aortic Diameters in Young Normotensive Patients With Turner Syndrome Without Structural Heart DiseasePubmed:29392348
  • Signature-oriented investigation of the efficacy of multicomponent drugs against heart failurePubmed: 30230922
  • Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension …Pubmed: 30359461
  • ANP/NPRA Inhibits Epithelial-Mesenchymal Transition of Airway by Targeting Smad3 in Asthma
  • Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure.
  • The poly (ADP-ribosyl) ation of BRD4 mediated by PARP1 promoted pathological cardiac hypertrophy34094834
  • Alarin moderated myocardial hypertrophy via inhibiting cyclic adenosine monophosphate/protein kinase A signaling pathway to attenuate autophagy34624429
  • Osteocrin alleviates cardiac hypertrophy via attenuating oxidative stressPubmed:35218795

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