Recombinant Brain Derived Neurotrophic Factor (BDNF)Homo sapiens (Human)Recombinant protein

Neurotrophin; Abrineurin

  • Product No.RPA011Hu01
  • Organism SpeciesHomo sapiens (Human)Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Predicted Molecular Mass39.0kDa
  • Accurate Molecular Mass39kDa(Analysis of differences refer to the manual)
  • Residues & TagsHis129~Arg247 with N-terminal GST Tag
  • Buffer Formulation20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • TraitsFreeze-dried powder
  • Purity> 95%
  • Isoelectric Point8.4
  • ApplicationsSDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
  • DownloadInstruction Manual
  • UOM10ug50ug200ug1mg1g
  • FOBUS$ 320US$ 800US$ 1600US$ 4800US$ 120000
  • Recombinant Brain Derived Neurotrophic Factor (BDNF)Package and Components
  • Recombinant Brain Derived Neurotrophic Factor (BDNF)Package and Components
  • Recombinant Brain Derived Neurotrophic Factor (BDNF)Gene sequencing
  • RPA011Hu01.jpgSDS-PAGE
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Reference

  • Toxicological SciencesInteraction of PFOS and BDE-47 Co-exposure on Thyroid Hormone Levels and TH-Related Gene and Protein Expression in Developing Rat BrainsPubMed: 21436126
  • Journal of the Neurological SciencesNeuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cordScienceDirect: S0022510X12001219
  • International Conference on BioscienceEffect of Benthosemapterotum Peptide on Aging in Mice with Oxidative DamagePsrcentre: 212059
  • Neuroscience LettersChronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone miceScienceDirect: S0304394010015788
  • BioMed Research InternationalNeuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord.NCBI: PMC3925622
  • Behavioural Brain ResearchThe effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factorPubmed:25446810
  • Neurochemical ResearchHeme Oxygenase-1 Protects Neurons from Ischemic Damage by UpreCavia (Guinea pig )lating Expression of Cu,Zn-SuperoxideDismutase, Catalase, and Brain-Derived Neurotrophic Factor in the Rabbit Spinal Cord.Pubmed:26559686
  • Exp Ther Med. Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young ratspmc:PMC4812310
  • Genet Mol ResChanges in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cellsPubmed:27323064

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