Recombinant Insulin (INS) Homo sapiens (Human) Recombinant protein

  • Product No.RPA448Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Predicted Molecular Mass41.8kDa
  • Accurate Molecular Mass41kDa(Analysis of differences refer to the manual)
  • Residues & TagsPhe25~Asn110 with N-terminal His and GST Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 97%
  • Isoelectric Point6.1
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 1g In stock
  • FOB US$ 140.00 FREE TRIAL US$ 350.00 FREE TRIAL US$ 700.00 FREE TRIAL US$ 2,100.00 FREE TRIAL US$ 52,500.00 FREE TRIAL
  • Quantity
  • Add to Cart Distributors
  • Recombinant Insulin (INS) Packages (Simulation)
  • Recombinant Insulin (INS) Packages (Simulation)
  • RPA448Hu01.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Neuroscience ResearchImpaired neural stem/progenitor cell proliferation in Streptozotocin-induced and spontaneous diabetic micePubMed: 20832431
  • International Journal of NanomedicineNicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathwayPubMed: PMC3770514
  • Mediators of InflammationMetformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK PhosphorylationPubmed: 24288442
  • American Journal of Research CommunicationPolo Like Kinase 1 (Plk1) Expression in Visceral Adipose Tissue of Morbidly Obese Non Diabetic WomenUsa-journals: Source
  • Nutr Hosp. Effect of the “protein diet” and bone tissue.Pubmed: 24483972
  • EndocrinologyRosiglitazone Inhibits Expression and Secretion of PEDF in Adipose Tissue and Liver of Male SD Rats Via a PPAR-γ Independent MechanismEndocrine: Source
  • CORRELA??ES ENTRE METABóLITOS E HORM?NIOS NO PERíODO DE TRANSI??O DE VACAS DE CORTE DE DIFERENTES GENóTIPOS CRIADAS NO SUL DO BRASIL Cnptia:Source
  • CONCENTRA??ES SéRICAS DE INSULINA NO PóS-PARTO RECENTE DE VACAS DE CORTE DE DIFERENTES GENóTIPOS CRIADAS NO SUL DO BRASILAlice:Source
  • Evid Based Complement Alternat Med.Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar RatsPubmed:Pmc4363645
  • Iran J Basic Med SciZataria multiflora increases insulin sensitivity and PPARγ gene expression in high fructose fed insulin resistant ratsPubmed:Pmc4046234
  • Biometals.?Perinatal low-dose iron treatment influences susceptibility to diet-induced adipogenesis in early-aged male Wistar ratsPubmed:24519243
  • Asian Pac J Cancer Prev.Effect of NUCKS-1 Overexpression on cytokine profiling in obese women with breast cancerPubmed:24568505
  • J Ethnopharmacol.?Antioxidative effects of Korean red ginseng in postmenopausal women: A double-blind randomized controlled trialPubmed:24814037
  • Obes SurgEffect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat ModelPubMed: 26202420
  • Evid Based Complement Alternat MedTinospora crispaPubMed: 25821506
  • international journal of biological and chemical sciencesTaraxacum officinaleIndex.Php: Ijbcs
  • NutritionStructural and functional abnormalities of hepatic tissues in male Wistar rats fed hyperwhey and super amino anabolic proteinPubmed:27158055
  • European Journal of PharmacologySubcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3尾 pathway in an amyloid 尾 protein induced alzheimer disease mouse modelPubmed:27131827
  • 
Archives of Physiology and BiochemistryImprovement of oral contraceptive-induced glucose dysreCavia (Guinea pig )lation and dyslipidemia by valproic acid is independent of circulating corticosteronePubmed:26855320
  • Archives of Physiology and BiochemistryActivation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorderPubmed:26934364
  • Cell and Tissue ResearchThe dynamic three-dimensional culture of islet-like clusters in decellularized liver scaffoldsPubmed:26796204
  • Adv Biomed Res.Trigonella foenum-graecum water extract improves insulin sensitivity and stimulates PPAR and γ gene expression in high fructose-fed insulin-resistant ratspmc:PMC4817393
  • Mech Ageing Dev. Central injection of GALR1 agonist M617 attenuates diabetic rat skeletal muscle insulin resistance through the Akt/AS160/GLUT4 pathwayPubmed:27041232
  • J Bone Miner ResFat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult RatsPubmed:26175082
  • Scientific ReportsMolecular characterization of insulin resistance and glycolytic metabolism in the rat uteruspubmed:27461373
  • International Journal of Medical Research ProfessionalsLow Circulating Vaspin Level is not associated with Insulinemic Status in Impaired Glucose Tolerant Subjectsprofile:Muhammad_Saiedullah
  • Journal of Pharmacy and Nutrition SciencesMetformin Reduces Oxidative Stress Status and Improves Plasma Insulin Level in Streptozotocin-Induced Diabetic Ratspms:index.php
  • EBioMedicine.Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.pubmed:28336389
  • Journal of Endocrinology and MetabolismCirculating Betatrophin and Hepatocyte Growth Factor in Type 2 Diabetic Patients: Their Relationship With Disease Prognosis405
  • American Journal of Translational ResearchSubcutaneous liraglutide ameliorates methylglyoxal-induced Alzheimer-like tau pathology and cognitive impairment by modulating tau hyperphosphorylation and glycogen synthase kinase-3β.pubmed:28337257
  • Journal of Molecular and Cellular CardiologyIrisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese micepubmed:27638193
  • Scientific ReportsThe anti-hyperglycemic efficacy of a lipid-lowering drug Daming capsule and the underlyingsignaling mechanisms in a rat model of diabetes mellitus.pubmed:27721485
  • Scientific ReportsAdiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway.pubmed:27678302
  • Molecular and Cellular EndocrinologyBaicalin against obesity and insulin resistance through activation of AKT/AS160/GLUT4 pathwaypubmed:28359800
  • DiabetologiaGinsenoside Rg5 attenuates hepatic glucagon response via suppression of succinate-associated HIF-1α induction in HFD-fed micepubmed:28280902
  • Journal of Diabetes ResearchHigh plasma glucagon levels correlate with waist-to-hip ratio, supra-iliac skinfold thickness, deep subcutaneous abdominal and intra-peritoneal adipose tissue …2376016.pdf

Recommend products