Recombinant Lecithin Cholesterol Acyltransferase (LCAT) 
phosphatidylcholine-sterol O-acyltransferase; Phospholipid-cholesterol acyltransferase
Overview
Properties
- Product No.RPJ516Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
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Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & Kinase
- Source Prokaryotic expression, Host E.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationSecreted
- Molecular Mass 20.7kDa, Accurate 22kDa(Analysis of differences refer to the manual)
- Residues & TagsGlu41~His210 with N-terminal His Tag
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder, Purity > 97%
- Isoelectric Point6.3
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Packages (Simulation)
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Packages (Simulation)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
Sequence
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Increment services
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Endotoxin Removal Kit
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BCA Protein Quantification Kit
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Protein Labeling Customized Service
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Molecular Mass Marker for Protein
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Recombinant Protein Customized Service
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Monoclonal Antibody Customized Service
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Polyclonal Antibody Customized Service
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Protein Activity Test Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Buffer
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Endotoxin Removal Kit II
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Real Time PCR Experimental Service
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
Citations
- Plasma phospholipase, γ‐CEHC and antioxidant capacity in fibromyalgiaPubMed: 26585319
- Alteration of Lysophosphatidylcholine-Related Metabolic Parameters in the Plasma of Mice withExperimental Sepsis.pubmed:28028754
- A combined biomarker panel shows improved sensitivity for the early detection of ovarian cancer allowing the identification of the most aggressive type II tumours.pubmed:28664912
- Lipid-lowering effects of medium-chain triglyceride-enriched coconut oil in combination with licorice extracts in experimental hyperlipidemic micePubmed: 30244576
- Identification and validation of differentially expressed proteins in serum of CSU patients with different duration of wheals using an iTRAQ labeling, 2D‑LC‑MS/MSDoi: 10.3892/etm.2018.6818
- Diagnosis of epithelial ovarian cancer using a combined protein biomarker panel.Pubmed: 31388184
- The synergistic mechanism of total saponins and flavonoids in Notoginseng− Safflower pair against myocardial ischemia uncovered by an integrated …Pubmed: 32739736
- Pharmacokinetics/pharmacometabolomics-pharmacodynamics reveals the synergistic mechanism of a multicomponent herbal formula, Baoyuan decoction?¡
- Defects in a liver-bone axis contribute to hepatic osteodystrophy disease progressionPubmed:35235775