Recombinant Mucin 1 (MUC1) Homo sapiens (Human) Recombinant protein

CD227; EMA; H23AG; KL-6; PEM; PEMT; PUM; CA15-3; CA153; Cancer antigen 15-3; Carcinoma-associated mucin; Episialin; Peanut-reactive urinary mucin; Polymorphic epithelial mucin

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane
  • Molecular Mass 17.2kDa, Accurate 17kDa(Analysis of differences refer to the manual)
  • Residues & TagsPro1034~Gln1151 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point6.1
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  • Recombinant Mucin 1 (MUC1) Packages (Simulation)
  • Recombinant Mucin 1 (MUC1) Packages (Simulation)
  • Recombinant Mucin 1 (MUC1) Figure . Gene Sequencing (extract)
  • RPA413Hu02.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas.PubMed: 20717734
  • Host Defense Proteins in Breast Milk and Neonatal Yeast ColonizationPubMed: 26116637
  • Activated Leukocyte Cell Adhesion Molecule (ALCAM) in Saudi Breast Cancer Patients as Prognostic and Predictive IndicatorPubMed: 26446295
  • The Presence and Anti-HIV-1 Function of Tenascin C in Breast Milk and Genital FluidsPubmed:27182834
  • The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghaifiles:ijcem0020163.pdf
  • Ultrasensitive detection of mucin 1 biomarker by immuno-loop-mediated isothermal amplification.pubmed:28107977
  • An adherent mucus layer attenuates the genotoxic effect of colibactin. pubmed:29156489
  • Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA10.21769:BioProtoc.2879
  • Aptasensors as a new sensing technology developed for the detection of MUC1 mucin: A review
  • A highly sensitive label-free electrochemical immunosensor based on AuNPs-PtNPs-MOFs for nuclear matrix protein 22 analysis in urine sample

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