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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Recombinant Protein Kinase N2 (PKN2)
PRK2; Pak-2; PRKCL2; Protein Kinase C-Like 2; Cardiolipin-Activated Protein Kinase Pak2
Organism species Homo sapiens (Human)
Product No.RPB987Hu01
SourceProkaryotic expression
HostE.coli
Purity> 95%
UOM50ug
Predicted Molecular Mass29.5kDa
Predicted isoelectric pointn/a
ApplicationsSDS-PAGE; WB; ELISA; IP.
Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
Subcellular Location n/a
ResiduesGlu67~Ile312 (Accession # Q16513) with N-terminal His-Tag
FormulationSupplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-ENLR KVTTDKKSLA YVDNILKKSN KKLEELHHKL QELNAHIVVS DPEDITDCPR TPDTPNNDPR CSTSNNRLKA LQKQLDIELK VKQGAENMIQ MYSNGSSKDR KLHGTAQQLL QDSKTKIEVI RMQILQAVQT NELAFDNAKP VISPLELRME ELRHHFRIEF AVAEGAKNVM KLLGSGKVTD RKALSEAQAR FNESSQKLDL LKYSLEQRLN EVPKNHPKSR IIIEELSLVA ASPTLSPRQS MI
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
PAB987Hu01Polyclonal Antibody to Protein Kinase N2 (PKN2)WB, ICC, IHC-P, IHC-F, ELISA
PAB987Hu02Polyclonal Antibody to Protein Kinase N2 (PKN2)WB, ICC, IHC-P, IHC-F, ELISA
PAB987Hu71Biotin-Linked Antibody to Protein Kinase N2 (PKN2)IHC;WB;ELISA
RPB987Hu01Recombinant Protein Kinase N2 (PKN2)SDS-PAGE; WB; ELISA; IP.
RPB987Hu02Recombinant Protein Kinase N2 (PKN2)SDS-PAGE; WB; ELISA; IP.